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Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis.

Salimon J, Abdullah BM, Salih N - Lipids Health Dis (2012)

Bottom Line: The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed.RSO did not show any toxic potential to the rats.This can be attributed no hazardous linamarin were found in RSO.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia. jumat@ukm.my

ABSTRACT

Background: The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.

Results: FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn't show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSO(h,) RSO(chl+mth) and RSO(eth) and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).

Conclusions: This can be attributed no hazardous linamarin were found in RSO.

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Molecular formula of linamarin.
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Figure 1: Molecular formula of linamarin.

Mentions: A linamarin is a cyanogenic glucoside [6]. The molar mass of linamarin is 247.21 g mol-1 and the density is 1.41 g·cm-3. The hydrolysis or cyanogensis of linamarin by endogenous enzyme, linamarase (β-glucosidase), results in the formation of glucose and acetone cyanohydrin, which later decomposes into hydrogen cyanide (HCN) and acetone [7,8]. The molecular formula of linamarin is (C10H17NO6) shown in Figure 1.


Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis.

Salimon J, Abdullah BM, Salih N - Lipids Health Dis (2012)

Molecular formula of linamarin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475106&req=5

Figure 1: Molecular formula of linamarin.
Mentions: A linamarin is a cyanogenic glucoside [6]. The molar mass of linamarin is 247.21 g mol-1 and the density is 1.41 g·cm-3. The hydrolysis or cyanogensis of linamarin by endogenous enzyme, linamarase (β-glucosidase), results in the formation of glucose and acetone cyanohydrin, which later decomposes into hydrogen cyanide (HCN) and acetone [7,8]. The molecular formula of linamarin is (C10H17NO6) shown in Figure 1.

Bottom Line: The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed.RSO did not show any toxic potential to the rats.This can be attributed no hazardous linamarin were found in RSO.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia. jumat@ukm.my

ABSTRACT

Background: The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.

Results: FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn't show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSO(h,) RSO(chl+mth) and RSO(eth) and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).

Conclusions: This can be attributed no hazardous linamarin were found in RSO.

Show MeSH
Related in: MedlinePlus