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Peripheral T-cell Lymphoma with Cyclin D1 overexpression: a case report.

Aquino G, Franco R, Ronconi F, Anniciello A, Russo L, De Chiara A, Panico L - Diagn Pathol (2012)

Bottom Line: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy.They show a complex karyotype without any specific genetic hallmark.Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Pathology Unit, National Cancer Institute Fondazione Giovanni Pascale, Via Mariano Semmola, 80131 Napoli, Italy.

ABSTRACT

Unlabelled: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy. They show a complex karyotype without any specific genetic hallmark. We report a case of peripheral T-cell lymphoma not otherwise specified with heterogeneous nuclear cyclin D1 immunohistochemical overexpression, due to gene copy gain, a phenomenon similar to that observed in mantle cell lymphoma characterized by t(11;14)(q13;q32). In this case report we underline the diagnostic pitfall represented by cyclin D1 immunohistochemical overexpression in a T-cell lymphoma. Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

Virtual slide: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1117747619703769.

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Peripheral T- cell Lymphoma infiltration, bone marrow biopsy: A) hematoxylin and eosin morphology 20X magnification;B) CD 3 Immunopositivity shows infiltration by neoplastic T cells 20X magnification.
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Figure 4: Peripheral T- cell Lymphoma infiltration, bone marrow biopsy: A) hematoxylin and eosin morphology 20X magnification;B) CD 3 Immunopositivity shows infiltration by neoplastic T cells 20X magnification.

Mentions: Although morphological and immunoprofile excluded a mantle cell lymphoma, chromosomal translocation (11; 14) (q13; q32) involving cyclin D1/IGH genes has been searched. FISH analysis for the detection of cyclin D1 status was performed using Vysis LSI IGH/CCND1 XT Dual Color Dual Fusion Probes (Vyses). This probe set uses the dual-color, dual fusion strategy and consists of a mixture of locus-specific fluorophore-labelled DNA probes containing sequences homologous to the IGH regions (Spectrum Green) and cyclin D1 breakpoint region (Spectrum Orange). The cyclin D1 contig is composed of three segments covering a region approximately of 942Kb locus 11q13 where are present different genes including cyclin D1. Green fluorescent spots represent Igh and red spots stand for cyclin D1. In a normal tissue we have two split signals of both colors while in a traslocated sample we have two or one fused signals (yellow). The cytogenetic analyses revealed a copy gain of the cyclin D1 without evidence of translocation because the sample shows two split signals of both colours (Figure3). Bone marrow biopsy showed a huge CD3+ T cell lymphoma infiltration (Figure4).


Peripheral T-cell Lymphoma with Cyclin D1 overexpression: a case report.

Aquino G, Franco R, Ronconi F, Anniciello A, Russo L, De Chiara A, Panico L - Diagn Pathol (2012)

Peripheral T- cell Lymphoma infiltration, bone marrow biopsy: A) hematoxylin and eosin morphology 20X magnification;B) CD 3 Immunopositivity shows infiltration by neoplastic T cells 20X magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475098&req=5

Figure 4: Peripheral T- cell Lymphoma infiltration, bone marrow biopsy: A) hematoxylin and eosin morphology 20X magnification;B) CD 3 Immunopositivity shows infiltration by neoplastic T cells 20X magnification.
Mentions: Although morphological and immunoprofile excluded a mantle cell lymphoma, chromosomal translocation (11; 14) (q13; q32) involving cyclin D1/IGH genes has been searched. FISH analysis for the detection of cyclin D1 status was performed using Vysis LSI IGH/CCND1 XT Dual Color Dual Fusion Probes (Vyses). This probe set uses the dual-color, dual fusion strategy and consists of a mixture of locus-specific fluorophore-labelled DNA probes containing sequences homologous to the IGH regions (Spectrum Green) and cyclin D1 breakpoint region (Spectrum Orange). The cyclin D1 contig is composed of three segments covering a region approximately of 942Kb locus 11q13 where are present different genes including cyclin D1. Green fluorescent spots represent Igh and red spots stand for cyclin D1. In a normal tissue we have two split signals of both colors while in a traslocated sample we have two or one fused signals (yellow). The cytogenetic analyses revealed a copy gain of the cyclin D1 without evidence of translocation because the sample shows two split signals of both colours (Figure3). Bone marrow biopsy showed a huge CD3+ T cell lymphoma infiltration (Figure4).

Bottom Line: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy.They show a complex karyotype without any specific genetic hallmark.Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Pathology Unit, National Cancer Institute Fondazione Giovanni Pascale, Via Mariano Semmola, 80131 Napoli, Italy.

ABSTRACT

Unlabelled: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy. They show a complex karyotype without any specific genetic hallmark. We report a case of peripheral T-cell lymphoma not otherwise specified with heterogeneous nuclear cyclin D1 immunohistochemical overexpression, due to gene copy gain, a phenomenon similar to that observed in mantle cell lymphoma characterized by t(11;14)(q13;q32). In this case report we underline the diagnostic pitfall represented by cyclin D1 immunohistochemical overexpression in a T-cell lymphoma. Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

Virtual slide: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1117747619703769.

Show MeSH
Related in: MedlinePlus