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Peripheral T-cell Lymphoma with Cyclin D1 overexpression: a case report.

Aquino G, Franco R, Ronconi F, Anniciello A, Russo L, De Chiara A, Panico L - Diagn Pathol (2012)

Bottom Line: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy.They show a complex karyotype without any specific genetic hallmark.Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Pathology Unit, National Cancer Institute Fondazione Giovanni Pascale, Via Mariano Semmola, 80131 Napoli, Italy.

ABSTRACT

Unlabelled: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy. They show a complex karyotype without any specific genetic hallmark. We report a case of peripheral T-cell lymphoma not otherwise specified with heterogeneous nuclear cyclin D1 immunohistochemical overexpression, due to gene copy gain, a phenomenon similar to that observed in mantle cell lymphoma characterized by t(11;14)(q13;q32). In this case report we underline the diagnostic pitfall represented by cyclin D1 immunohistochemical overexpression in a T-cell lymphoma. Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

Virtual slide: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1117747619703769.

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FISH analysis using a IGH/ CCND1 t(11;14) probe and Clonality results in 2% agarose gel (A) and (B) Green fluorescent spots represent Igh and red spots stand for CCND1. Both pictures show distinct red and green signals (split signals indicating no translocation) and an increase red signals (cyclin D1 copy gain) at different magnification 63x and 100x respectively. C) Analysis of results of B Clonality in 2% agarose gel 1) FR1-JH monoclonal B control. 2) Sample. 3) FR1-JH polyclonal B control. 4) FR2-JH monoclonal B control. 5) sample. 6) FR2-JH polyclonal B control. 7) FR3-JH monoclonal B control. 8) sample. 9) FR3-JH polyclonal B control. D) Analysis of results of T Clonality in 2% agarose gel 1)VJ-A monoclonal T control. 2) sample. 3) VJ-A polyclonal control. 4)VJ-B monoclonal T control. 5) sample. 6) VJ-B polyclonal control. 7) Beta-Actin Control.
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Figure 3: FISH analysis using a IGH/ CCND1 t(11;14) probe and Clonality results in 2% agarose gel (A) and (B) Green fluorescent spots represent Igh and red spots stand for CCND1. Both pictures show distinct red and green signals (split signals indicating no translocation) and an increase red signals (cyclin D1 copy gain) at different magnification 63x and 100x respectively. C) Analysis of results of B Clonality in 2% agarose gel 1) FR1-JH monoclonal B control. 2) Sample. 3) FR1-JH polyclonal B control. 4) FR2-JH monoclonal B control. 5) sample. 6) FR2-JH polyclonal B control. 7) FR3-JH monoclonal B control. 8) sample. 9) FR3-JH polyclonal B control. D) Analysis of results of T Clonality in 2% agarose gel 1)VJ-A monoclonal T control. 2) sample. 3) VJ-A polyclonal control. 4)VJ-B monoclonal T control. 5) sample. 6) VJ-B polyclonal control. 7) Beta-Actin Control.

Mentions: Moreover molecular analysis of IgH and TCR rearrangement was done. In particular detection of B clonality was investigated by identification of VDJ segments amplification of the hypervariable region of immunoglobulin heavy chain (IgH) using multiple primers complementary to conserved regions in the involved gene (Nanogen-Master Diagnòstica); the detection of T clonality was investigated by identification of VJ segments amplification of TCRgamma gene using primers complementary flanking regions of the V and the J segments (Nanogen-Master Diagnòstica). In electrophoresis study, clonal rearrangement of TCR gamma gene is shown by the presence of a single strong sharp band within the expected size range from clonal control ( Figure3). Our PCR analysis definitively demonstrated neoplastic T cell proliferation, being clonally rearranged for TCRgamma gene, in particular our sample presents VJ-B rearrangement as shown from band approximately for 215bp (Figure3).


Peripheral T-cell Lymphoma with Cyclin D1 overexpression: a case report.

Aquino G, Franco R, Ronconi F, Anniciello A, Russo L, De Chiara A, Panico L - Diagn Pathol (2012)

FISH analysis using a IGH/ CCND1 t(11;14) probe and Clonality results in 2% agarose gel (A) and (B) Green fluorescent spots represent Igh and red spots stand for CCND1. Both pictures show distinct red and green signals (split signals indicating no translocation) and an increase red signals (cyclin D1 copy gain) at different magnification 63x and 100x respectively. C) Analysis of results of B Clonality in 2% agarose gel 1) FR1-JH monoclonal B control. 2) Sample. 3) FR1-JH polyclonal B control. 4) FR2-JH monoclonal B control. 5) sample. 6) FR2-JH polyclonal B control. 7) FR3-JH monoclonal B control. 8) sample. 9) FR3-JH polyclonal B control. D) Analysis of results of T Clonality in 2% agarose gel 1)VJ-A monoclonal T control. 2) sample. 3) VJ-A polyclonal control. 4)VJ-B monoclonal T control. 5) sample. 6) VJ-B polyclonal control. 7) Beta-Actin Control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475098&req=5

Figure 3: FISH analysis using a IGH/ CCND1 t(11;14) probe and Clonality results in 2% agarose gel (A) and (B) Green fluorescent spots represent Igh and red spots stand for CCND1. Both pictures show distinct red and green signals (split signals indicating no translocation) and an increase red signals (cyclin D1 copy gain) at different magnification 63x and 100x respectively. C) Analysis of results of B Clonality in 2% agarose gel 1) FR1-JH monoclonal B control. 2) Sample. 3) FR1-JH polyclonal B control. 4) FR2-JH monoclonal B control. 5) sample. 6) FR2-JH polyclonal B control. 7) FR3-JH monoclonal B control. 8) sample. 9) FR3-JH polyclonal B control. D) Analysis of results of T Clonality in 2% agarose gel 1)VJ-A monoclonal T control. 2) sample. 3) VJ-A polyclonal control. 4)VJ-B monoclonal T control. 5) sample. 6) VJ-B polyclonal control. 7) Beta-Actin Control.
Mentions: Moreover molecular analysis of IgH and TCR rearrangement was done. In particular detection of B clonality was investigated by identification of VDJ segments amplification of the hypervariable region of immunoglobulin heavy chain (IgH) using multiple primers complementary to conserved regions in the involved gene (Nanogen-Master Diagnòstica); the detection of T clonality was investigated by identification of VJ segments amplification of TCRgamma gene using primers complementary flanking regions of the V and the J segments (Nanogen-Master Diagnòstica). In electrophoresis study, clonal rearrangement of TCR gamma gene is shown by the presence of a single strong sharp band within the expected size range from clonal control ( Figure3). Our PCR analysis definitively demonstrated neoplastic T cell proliferation, being clonally rearranged for TCRgamma gene, in particular our sample presents VJ-B rearrangement as shown from band approximately for 215bp (Figure3).

Bottom Line: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy.They show a complex karyotype without any specific genetic hallmark.Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Pathology Unit, National Cancer Institute Fondazione Giovanni Pascale, Via Mariano Semmola, 80131 Napoli, Italy.

ABSTRACT

Unlabelled: Peripheral T-cell lymphomas not otherwise specified are generally considered aggressive non-Hodgkin lymphomas, because of poor natural outcome and response to therapy. They show a complex karyotype without any specific genetic hallmark. We report a case of peripheral T-cell lymphoma not otherwise specified with heterogeneous nuclear cyclin D1 immunohistochemical overexpression, due to gene copy gain, a phenomenon similar to that observed in mantle cell lymphoma characterized by t(11;14)(q13;q32). In this case report we underline the diagnostic pitfall represented by cyclin D1 immunohistochemical overexpression in a T-cell lymphoma. Several pitfalls could lead to misinterpretation of diagnosis, therefore, we underlined the need to integrate the classical histology and immunohistochemistry with molecular tests as clonality or fluorescence in situ hybridization.

Virtual slide: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1117747619703769.

Show MeSH
Related in: MedlinePlus