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Chemical composition of 8 eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities.

Elaissi A, Rouis Z, Salem NA, Mabrouk S, ben Salem Y, Salah KB, Aouni M, Farhat F, Chemli R, Harzallah-Skhiri F, Khouja ML - BMC Complement Altern Med (2012)

Bottom Line: The cytotoxic effect and the antiviral activity varied significantly within Eucalyptus species oils.In addition, E. odorata oil showed the most cytotoxic effect.However, the best antiviral activity appeared with E. bicostata.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, Monastir 5019, Tunisia. aelaissi@yahoo.fr

ABSTRACT

Background: In 1957, Tunisia introduced 117 species of Eucalyptus; they have been used as fire wood, for the production of mine wood and to fight erosion. Actually, Eucalyptus essential oil is traditionally used to treat respiratory tract disorders such as pharyngitis, bronchitis, and sinusitis. A few investigations were reported on the biological activities of Eucalyptus oils worldwide. In Tunisia, our previous works conducted in 2010 and 2011 had been the first reports to study the antibacterial activities against reference strains. At that time it was not possible to evaluate their antimicrobial activities against clinical bacterial strains and other pathogens such as virus and fungi.

Methods: The essential oils of eight Eucalyptus species harvested from the Jbel Abderrahman, Korbous (North East Tunisia) and Souinet arboreta (North of Tunisia) were evaluated for their antimicrobial activities by disc diffusion and microbroth dilution methods against seven bacterial isolates: Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pneumoniae and Streptococcus pyogenes. In addition, the bactericidal, fungicidal and the antiviral activities of the tested oils were carried out.

Results: Twenty five components were identified by GC/FID and GC/MS. These components were used to correlate with the biological activities of the tested oils. The chemical principal component analysis identified three groups, each of them constituted a chemotype. According to the values of zone diameter and percentage of the inhibition (zdi, % I, respectively), four groups and subgroups of bacterial strains and three groups of fungal strains were characterized by their sensitivity levels to Eucalyptus oils. The cytotoxic effect and the antiviral activity varied significantly within Eucalyptus species oils.

Conclusions: E. odorata showed the strongest activity against S. aureus, H. influenzae, S. agalactiae, S. pyogenes, S. pneumoniae and against all the tested fungal strains. In addition, E. odorata oil showed the most cytotoxic effect. However, the best antiviral activity appeared with E. bicostata. Virus pretreatment with E. bicostata essential oil showed better antiviral activity (IC(50) = 0.7 mg/ml, SI = 22.8) than cell-pretreatment (IC(50) = 4.8 mg/ml, SI = 3.33). The essential oil of E. astringens showed antiviral activity only when incubated with virus prior to cell infection. This activity was dose-dependent and the antiviral activity diminished with the decreasing essential oil concentration.

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PCA of the antifungal activity of 0 8 Eucalyptusessential oils species against five fungi (♦): T. rub  =  Trichophyton rubrum, T. sou = Trichophyton soudanense, M. can = Microsporum canis; S. bre = Scopulariopsis brevicaulis, C. alb = Candida albicans. For the abbreviation of the Eucalyptus species (▴) (see Table 1).
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Figure 5: PCA of the antifungal activity of 0 8 Eucalyptusessential oils species against five fungi (♦): T. rub  =  Trichophyton rubrum, T. sou = Trichophyton soudanense, M. can = Microsporum canis; S. bre = Scopulariopsis brevicaulis, C. alb = Candida albicans. For the abbreviation of the Eucalyptus species (▴) (see Table 1).

Mentions: The PCA horizontal axis explained 73% of the total variance, while the vertical axis a further 14.15% (Figure 5). The HCA indicated three groups of species (A, B and C) with a dissimilarity ≥ 5.0 (Figure 6).


Chemical composition of 8 eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities.

Elaissi A, Rouis Z, Salem NA, Mabrouk S, ben Salem Y, Salah KB, Aouni M, Farhat F, Chemli R, Harzallah-Skhiri F, Khouja ML - BMC Complement Altern Med (2012)

PCA of the antifungal activity of 0 8 Eucalyptusessential oils species against five fungi (♦): T. rub  =  Trichophyton rubrum, T. sou = Trichophyton soudanense, M. can = Microsporum canis; S. bre = Scopulariopsis brevicaulis, C. alb = Candida albicans. For the abbreviation of the Eucalyptus species (▴) (see Table 1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475086&req=5

Figure 5: PCA of the antifungal activity of 0 8 Eucalyptusessential oils species against five fungi (♦): T. rub  =  Trichophyton rubrum, T. sou = Trichophyton soudanense, M. can = Microsporum canis; S. bre = Scopulariopsis brevicaulis, C. alb = Candida albicans. For the abbreviation of the Eucalyptus species (▴) (see Table 1).
Mentions: The PCA horizontal axis explained 73% of the total variance, while the vertical axis a further 14.15% (Figure 5). The HCA indicated three groups of species (A, B and C) with a dissimilarity ≥ 5.0 (Figure 6).

Bottom Line: The cytotoxic effect and the antiviral activity varied significantly within Eucalyptus species oils.In addition, E. odorata oil showed the most cytotoxic effect.However, the best antiviral activity appeared with E. bicostata.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, Monastir 5019, Tunisia. aelaissi@yahoo.fr

ABSTRACT

Background: In 1957, Tunisia introduced 117 species of Eucalyptus; they have been used as fire wood, for the production of mine wood and to fight erosion. Actually, Eucalyptus essential oil is traditionally used to treat respiratory tract disorders such as pharyngitis, bronchitis, and sinusitis. A few investigations were reported on the biological activities of Eucalyptus oils worldwide. In Tunisia, our previous works conducted in 2010 and 2011 had been the first reports to study the antibacterial activities against reference strains. At that time it was not possible to evaluate their antimicrobial activities against clinical bacterial strains and other pathogens such as virus and fungi.

Methods: The essential oils of eight Eucalyptus species harvested from the Jbel Abderrahman, Korbous (North East Tunisia) and Souinet arboreta (North of Tunisia) were evaluated for their antimicrobial activities by disc diffusion and microbroth dilution methods against seven bacterial isolates: Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pneumoniae and Streptococcus pyogenes. In addition, the bactericidal, fungicidal and the antiviral activities of the tested oils were carried out.

Results: Twenty five components were identified by GC/FID and GC/MS. These components were used to correlate with the biological activities of the tested oils. The chemical principal component analysis identified three groups, each of them constituted a chemotype. According to the values of zone diameter and percentage of the inhibition (zdi, % I, respectively), four groups and subgroups of bacterial strains and three groups of fungal strains were characterized by their sensitivity levels to Eucalyptus oils. The cytotoxic effect and the antiviral activity varied significantly within Eucalyptus species oils.

Conclusions: E. odorata showed the strongest activity against S. aureus, H. influenzae, S. agalactiae, S. pyogenes, S. pneumoniae and against all the tested fungal strains. In addition, E. odorata oil showed the most cytotoxic effect. However, the best antiviral activity appeared with E. bicostata. Virus pretreatment with E. bicostata essential oil showed better antiviral activity (IC(50) = 0.7 mg/ml, SI = 22.8) than cell-pretreatment (IC(50) = 4.8 mg/ml, SI = 3.33). The essential oil of E. astringens showed antiviral activity only when incubated with virus prior to cell infection. This activity was dose-dependent and the antiviral activity diminished with the decreasing essential oil concentration.

Show MeSH
Related in: MedlinePlus