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Biological activities of extracts from Chenopodium ambrosioides Lineu and Kielmeyera neglecta Saddi.

Sousa ZL, de Oliveira FF, da Conceição AO, Silva LA, Rossi MH, Santos Jda S, Andrioli JL - Ann. Clin. Microbiol. Antimicrob. (2012)

Bottom Line: Extracts from C. ambrosioides (Hex, DCM and EtOH) and K. neglecta (EtOAc and EtOH) showed high bioactivity against A. salina (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells.C. ambrosioides Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against Candida krusei (MIC = 100 μg/mL).Generally speaking, extracts from C. ambrosioides and K. neglecta showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Pós-graduação em Biologia e Biotecnologia de Microrganismos, Universidade Estadual de Santa Cruz, Campus Prof, Soane Nazaré de Andrade, Km 16 - Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil.

ABSTRACT

Background: Chenopodium ambrosioides and Kielmeyera neglecta are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds.

Methods: Extracts from these plants were extracted with hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH) and assessed for their antimicrobial properties, bioactivity against Artemia salina Leach and antifungal action on the cell wall of Neurospora crassa.

Results: Extracts from C. ambrosioides (Hex, DCM and EtOH) and K. neglecta (EtOAc and EtOH) showed high bioactivity against A. salina (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells. C. ambrosioides Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against Candida krusei (MIC = 100 μg/mL). By comparing the inhibitory concentration of 50% growth (IC 50%) with the growth control, extracts from K. neglecta EtOAc and EtOH have shown activities against multidrug-resistant bacteria (Enterococcus faecalis ATCC 51299 and Staphylococcus aureus ATCC 43300), with IC 50% of 12.5 μg/mL The assay carried out on N. crassa allowed defining that extracts with antifungal activity do not have action through inhibition of cell wall synthesis.

Conclusions: Generally speaking, extracts from C. ambrosioides and K. neglecta showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.

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Mortality of Artemia salina to extracts of K. neglecta (KN) and C. ambrosioides (CA); Hex, hexane; DCM, dichloromethane; EtOAc, ethyl acetate.
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Figure 1: Mortality of Artemia salina to extracts of K. neglecta (KN) and C. ambrosioides (CA); Hex, hexane; DCM, dichloromethane; EtOAc, ethyl acetate.

Mentions: The crustacean A. salina is highly sensitive to a variety of compounds [37] and is considered to be a useful screening tool of active substances in bioassay [4,31]; furthermore, it has good correlation with cytotoxicity in human cancer cells [31,38]. As shown in Table 1, results indicate high bioactivity (LC50 < 1000 μg/mL) for extracts from K. neglecta EtOAc and EtOH, and C. ambrosioides DCM and EtOH were considered as active [31,39,40]. As compared with the other tested extracts, C. ambrosioides Hex showed very high mortality rate (Figure 1) and thereby made it statistically impossible to calculate the LC50, but was considered to be an active extract. Bioactivity in 6 h was only high for C. ambrosioides Hex, reinforcing the possibility for this extract to contain bioactive compounds.


Biological activities of extracts from Chenopodium ambrosioides Lineu and Kielmeyera neglecta Saddi.

Sousa ZL, de Oliveira FF, da Conceição AO, Silva LA, Rossi MH, Santos Jda S, Andrioli JL - Ann. Clin. Microbiol. Antimicrob. (2012)

Mortality of Artemia salina to extracts of K. neglecta (KN) and C. ambrosioides (CA); Hex, hexane; DCM, dichloromethane; EtOAc, ethyl acetate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475072&req=5

Figure 1: Mortality of Artemia salina to extracts of K. neglecta (KN) and C. ambrosioides (CA); Hex, hexane; DCM, dichloromethane; EtOAc, ethyl acetate.
Mentions: The crustacean A. salina is highly sensitive to a variety of compounds [37] and is considered to be a useful screening tool of active substances in bioassay [4,31]; furthermore, it has good correlation with cytotoxicity in human cancer cells [31,38]. As shown in Table 1, results indicate high bioactivity (LC50 < 1000 μg/mL) for extracts from K. neglecta EtOAc and EtOH, and C. ambrosioides DCM and EtOH were considered as active [31,39,40]. As compared with the other tested extracts, C. ambrosioides Hex showed very high mortality rate (Figure 1) and thereby made it statistically impossible to calculate the LC50, but was considered to be an active extract. Bioactivity in 6 h was only high for C. ambrosioides Hex, reinforcing the possibility for this extract to contain bioactive compounds.

Bottom Line: Extracts from C. ambrosioides (Hex, DCM and EtOH) and K. neglecta (EtOAc and EtOH) showed high bioactivity against A. salina (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells.C. ambrosioides Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against Candida krusei (MIC = 100 μg/mL).Generally speaking, extracts from C. ambrosioides and K. neglecta showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Pós-graduação em Biologia e Biotecnologia de Microrganismos, Universidade Estadual de Santa Cruz, Campus Prof, Soane Nazaré de Andrade, Km 16 - Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil.

ABSTRACT

Background: Chenopodium ambrosioides and Kielmeyera neglecta are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds.

Methods: Extracts from these plants were extracted with hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH) and assessed for their antimicrobial properties, bioactivity against Artemia salina Leach and antifungal action on the cell wall of Neurospora crassa.

Results: Extracts from C. ambrosioides (Hex, DCM and EtOH) and K. neglecta (EtOAc and EtOH) showed high bioactivity against A. salina (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells. C. ambrosioides Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against Candida krusei (MIC = 100 μg/mL). By comparing the inhibitory concentration of 50% growth (IC 50%) with the growth control, extracts from K. neglecta EtOAc and EtOH have shown activities against multidrug-resistant bacteria (Enterococcus faecalis ATCC 51299 and Staphylococcus aureus ATCC 43300), with IC 50% of 12.5 μg/mL The assay carried out on N. crassa allowed defining that extracts with antifungal activity do not have action through inhibition of cell wall synthesis.

Conclusions: Generally speaking, extracts from C. ambrosioides and K. neglecta showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.

Show MeSH
Related in: MedlinePlus