Limits...
The effects of berberine on hyperhomocysteinemia and hyperlipidemia in rats fed with a long-term high-fat diet.

Chang XX, Yan HM, Xu Q, Xia MF, Bian H, Zhu TF, Gao X - Lipids Health Dis (2012)

Bottom Line: The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.In parallel, it also decreased body weight and improved serum TC and LDL-c.Berberine also tended to decrease hepatic cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT

Background: The study was undertaken to examine the effects of berberine (BBR) on serum homocysteine, lipids and the aortic lesion in Sprague-Dawley (SD) rats fed with a long-term high-fat diet (HFD).

Methods: Healthy male SD rats weighing 190-210 g received randomly standard diet or a high-fat diet for 24 weeks. After 8 weeks of feeding, rats fed with HFD were randomized to receive berberine (200 mg · kg-1· day-1) or vehicle by gavage for 16 weeks. After overnight fasting, all rats were sacrificed and total blood samples were also collected for determinant of fasting serum homocysteine (Hcy), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels. The aorta was stained with hematoxylin and eosin (HE) and Sudan Ш to evaluate aortic lesion. The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.

Results: Intragastrical administration with berberine for 16 weeks lowered serum Hcy in rats fed with a high-fat diet. In parallel, it also decreased body weight and improved serum TC and LDL-c. Berberine also tended to decrease hepatic cholesterol. Consistently, berberine also upregulated LDL receptor (LDLR) mRNA level and suppressed HMGR gene expression. Meanwhile, upon berberine-treated rats, there was a significant increase in apolipoprotein E (apoE) mRNA, but no change in apoAI and scavenger receptor (SR) mRNA in the liver. Further, no atherosclerotic lesions were developed in berberine-treated rats for 16 weeks.

Conclusion: Berberine can counteract HFD-elicited hyperhomocysteinemia and hyperlipidemia partially via upregulating LDLR and apoE mRNA levels and suppressing HMGR gene expression.

Show MeSH

Related in: MedlinePlus

Effects of berberine (BBR) on the aorta of rats fed with a high-fat diet. After BBR treatment for 16 weeks, rats were killed after a 14-hour fast. Histological analysis of the aorta of the HFD-fed rats treated with vehicle (C group) or BBR (B group). The aorta sections were stained with hematoxylin and eosin (A-D) or with Sudan Ш (E-I) to evaluate the pathologic structures. Representative photographs are shown. A-B, E-F: Photographs are at × 50 magnification, C-D, G-H: Photographs are at × 400 magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3475055&req=5

Figure 5: Effects of berberine (BBR) on the aorta of rats fed with a high-fat diet. After BBR treatment for 16 weeks, rats were killed after a 14-hour fast. Histological analysis of the aorta of the HFD-fed rats treated with vehicle (C group) or BBR (B group). The aorta sections were stained with hematoxylin and eosin (A-D) or with Sudan Ш (E-I) to evaluate the pathologic structures. Representative photographs are shown. A-B, E-F: Photographs are at × 50 magnification, C-D, G-H: Photographs are at × 400 magnification.

Mentions: Histological analysis by HE staining of the aorta showed that the tunica intima of aorta remained smooth and intact in rats with a 24- weeks high-fat diet after treatment with berberine for 16 weeks (Figure 5A-D). The results of aorta stained with Sudan III showed that no lipid inclusions and foam cell accumulation were seen in the aortic endothelium of these rats (Figure 5E-H). Furthermore, in other rats fed with a high-fat diet without administration of berberine, there were also no deposits of monocyte-derived macrophages and abnormal fatty droplets in aortic tunica intima. These results suggested that no atherosclerotic lesions were developed in berberine-treated for 16 weeks and control rats fed with a long-term high-fat diet.


The effects of berberine on hyperhomocysteinemia and hyperlipidemia in rats fed with a long-term high-fat diet.

Chang XX, Yan HM, Xu Q, Xia MF, Bian H, Zhu TF, Gao X - Lipids Health Dis (2012)

Effects of berberine (BBR) on the aorta of rats fed with a high-fat diet. After BBR treatment for 16 weeks, rats were killed after a 14-hour fast. Histological analysis of the aorta of the HFD-fed rats treated with vehicle (C group) or BBR (B group). The aorta sections were stained with hematoxylin and eosin (A-D) or with Sudan Ш (E-I) to evaluate the pathologic structures. Representative photographs are shown. A-B, E-F: Photographs are at × 50 magnification, C-D, G-H: Photographs are at × 400 magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475055&req=5

Figure 5: Effects of berberine (BBR) on the aorta of rats fed with a high-fat diet. After BBR treatment for 16 weeks, rats were killed after a 14-hour fast. Histological analysis of the aorta of the HFD-fed rats treated with vehicle (C group) or BBR (B group). The aorta sections were stained with hematoxylin and eosin (A-D) or with Sudan Ш (E-I) to evaluate the pathologic structures. Representative photographs are shown. A-B, E-F: Photographs are at × 50 magnification, C-D, G-H: Photographs are at × 400 magnification.
Mentions: Histological analysis by HE staining of the aorta showed that the tunica intima of aorta remained smooth and intact in rats with a 24- weeks high-fat diet after treatment with berberine for 16 weeks (Figure 5A-D). The results of aorta stained with Sudan III showed that no lipid inclusions and foam cell accumulation were seen in the aortic endothelium of these rats (Figure 5E-H). Furthermore, in other rats fed with a high-fat diet without administration of berberine, there were also no deposits of monocyte-derived macrophages and abnormal fatty droplets in aortic tunica intima. These results suggested that no atherosclerotic lesions were developed in berberine-treated for 16 weeks and control rats fed with a long-term high-fat diet.

Bottom Line: The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.In parallel, it also decreased body weight and improved serum TC and LDL-c.Berberine also tended to decrease hepatic cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT

Background: The study was undertaken to examine the effects of berberine (BBR) on serum homocysteine, lipids and the aortic lesion in Sprague-Dawley (SD) rats fed with a long-term high-fat diet (HFD).

Methods: Healthy male SD rats weighing 190-210 g received randomly standard diet or a high-fat diet for 24 weeks. After 8 weeks of feeding, rats fed with HFD were randomized to receive berberine (200 mg · kg-1· day-1) or vehicle by gavage for 16 weeks. After overnight fasting, all rats were sacrificed and total blood samples were also collected for determinant of fasting serum homocysteine (Hcy), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels. The aorta was stained with hematoxylin and eosin (HE) and Sudan Ш to evaluate aortic lesion. The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.

Results: Intragastrical administration with berberine for 16 weeks lowered serum Hcy in rats fed with a high-fat diet. In parallel, it also decreased body weight and improved serum TC and LDL-c. Berberine also tended to decrease hepatic cholesterol. Consistently, berberine also upregulated LDL receptor (LDLR) mRNA level and suppressed HMGR gene expression. Meanwhile, upon berberine-treated rats, there was a significant increase in apolipoprotein E (apoE) mRNA, but no change in apoAI and scavenger receptor (SR) mRNA in the liver. Further, no atherosclerotic lesions were developed in berberine-treated rats for 16 weeks.

Conclusion: Berberine can counteract HFD-elicited hyperhomocysteinemia and hyperlipidemia partially via upregulating LDLR and apoE mRNA levels and suppressing HMGR gene expression.

Show MeSH
Related in: MedlinePlus