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The effects of berberine on hyperhomocysteinemia and hyperlipidemia in rats fed with a long-term high-fat diet.

Chang XX, Yan HM, Xu Q, Xia MF, Bian H, Zhu TF, Gao X - Lipids Health Dis (2012)

Bottom Line: The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.In parallel, it also decreased body weight and improved serum TC and LDL-c.Berberine also tended to decrease hepatic cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT

Background: The study was undertaken to examine the effects of berberine (BBR) on serum homocysteine, lipids and the aortic lesion in Sprague-Dawley (SD) rats fed with a long-term high-fat diet (HFD).

Methods: Healthy male SD rats weighing 190-210 g received randomly standard diet or a high-fat diet for 24 weeks. After 8 weeks of feeding, rats fed with HFD were randomized to receive berberine (200 mg · kg-1· day-1) or vehicle by gavage for 16 weeks. After overnight fasting, all rats were sacrificed and total blood samples were also collected for determinant of fasting serum homocysteine (Hcy), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels. The aorta was stained with hematoxylin and eosin (HE) and Sudan Ш to evaluate aortic lesion. The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.

Results: Intragastrical administration with berberine for 16 weeks lowered serum Hcy in rats fed with a high-fat diet. In parallel, it also decreased body weight and improved serum TC and LDL-c. Berberine also tended to decrease hepatic cholesterol. Consistently, berberine also upregulated LDL receptor (LDLR) mRNA level and suppressed HMGR gene expression. Meanwhile, upon berberine-treated rats, there was a significant increase in apolipoprotein E (apoE) mRNA, but no change in apoAI and scavenger receptor (SR) mRNA in the liver. Further, no atherosclerotic lesions were developed in berberine-treated rats for 16 weeks.

Conclusion: Berberine can counteract HFD-elicited hyperhomocysteinemia and hyperlipidemia partially via upregulating LDLR and apoE mRNA levels and suppressing HMGR gene expression.

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Related in: MedlinePlus

Effects of berberine (BBR) on serum Hcy and lipid profile. Serum Hcy (A), TC (B) and LDL-c (C) are the average of each group (n = 8). N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.
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Figure 2: Effects of berberine (BBR) on serum Hcy and lipid profile. Serum Hcy (A), TC (B) and LDL-c (C) are the average of each group (n = 8). N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.

Mentions: Intragastrical administration with BBR for 16 weeks, serum homocysteine was significantly decreased by about 60% in contrast to the vehicle-treated rats fed with the same high-fat diet (p < 0.001, Figure 2A). BBR treatment significantly lowered the levels of serum TC (p < 0.05, Figure 2B) and LDL-c (p < 0.05, Figure 2C) as compared with the placebo group in SD rats fed with a high-fat diet. These data suggested that BBR exert antagonizing effects on HFD-induced dyslipidemia and hyperhomocysteinemia in SD rats.


The effects of berberine on hyperhomocysteinemia and hyperlipidemia in rats fed with a long-term high-fat diet.

Chang XX, Yan HM, Xu Q, Xia MF, Bian H, Zhu TF, Gao X - Lipids Health Dis (2012)

Effects of berberine (BBR) on serum Hcy and lipid profile. Serum Hcy (A), TC (B) and LDL-c (C) are the average of each group (n = 8). N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475055&req=5

Figure 2: Effects of berberine (BBR) on serum Hcy and lipid profile. Serum Hcy (A), TC (B) and LDL-c (C) are the average of each group (n = 8). N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.
Mentions: Intragastrical administration with BBR for 16 weeks, serum homocysteine was significantly decreased by about 60% in contrast to the vehicle-treated rats fed with the same high-fat diet (p < 0.001, Figure 2A). BBR treatment significantly lowered the levels of serum TC (p < 0.05, Figure 2B) and LDL-c (p < 0.05, Figure 2C) as compared with the placebo group in SD rats fed with a high-fat diet. These data suggested that BBR exert antagonizing effects on HFD-induced dyslipidemia and hyperhomocysteinemia in SD rats.

Bottom Line: The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.In parallel, it also decreased body weight and improved serum TC and LDL-c.Berberine also tended to decrease hepatic cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT

Background: The study was undertaken to examine the effects of berberine (BBR) on serum homocysteine, lipids and the aortic lesion in Sprague-Dawley (SD) rats fed with a long-term high-fat diet (HFD).

Methods: Healthy male SD rats weighing 190-210 g received randomly standard diet or a high-fat diet for 24 weeks. After 8 weeks of feeding, rats fed with HFD were randomized to receive berberine (200 mg · kg-1· day-1) or vehicle by gavage for 16 weeks. After overnight fasting, all rats were sacrificed and total blood samples were also collected for determinant of fasting serum homocysteine (Hcy), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels. The aorta was stained with hematoxylin and eosin (HE) and Sudan Ш to evaluate aortic lesion. The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.

Results: Intragastrical administration with berberine for 16 weeks lowered serum Hcy in rats fed with a high-fat diet. In parallel, it also decreased body weight and improved serum TC and LDL-c. Berberine also tended to decrease hepatic cholesterol. Consistently, berberine also upregulated LDL receptor (LDLR) mRNA level and suppressed HMGR gene expression. Meanwhile, upon berberine-treated rats, there was a significant increase in apolipoprotein E (apoE) mRNA, but no change in apoAI and scavenger receptor (SR) mRNA in the liver. Further, no atherosclerotic lesions were developed in berberine-treated rats for 16 weeks.

Conclusion: Berberine can counteract HFD-elicited hyperhomocysteinemia and hyperlipidemia partially via upregulating LDLR and apoE mRNA levels and suppressing HMGR gene expression.

Show MeSH
Related in: MedlinePlus