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The effects of berberine on hyperhomocysteinemia and hyperlipidemia in rats fed with a long-term high-fat diet.

Chang XX, Yan HM, Xu Q, Xia MF, Bian H, Zhu TF, Gao X - Lipids Health Dis (2012)

Bottom Line: The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.In parallel, it also decreased body weight and improved serum TC and LDL-c.Berberine also tended to decrease hepatic cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT

Background: The study was undertaken to examine the effects of berberine (BBR) on serum homocysteine, lipids and the aortic lesion in Sprague-Dawley (SD) rats fed with a long-term high-fat diet (HFD).

Methods: Healthy male SD rats weighing 190-210 g received randomly standard diet or a high-fat diet for 24 weeks. After 8 weeks of feeding, rats fed with HFD were randomized to receive berberine (200 mg · kg-1· day-1) or vehicle by gavage for 16 weeks. After overnight fasting, all rats were sacrificed and total blood samples were also collected for determinant of fasting serum homocysteine (Hcy), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels. The aorta was stained with hematoxylin and eosin (HE) and Sudan Ш to evaluate aortic lesion. The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.

Results: Intragastrical administration with berberine for 16 weeks lowered serum Hcy in rats fed with a high-fat diet. In parallel, it also decreased body weight and improved serum TC and LDL-c. Berberine also tended to decrease hepatic cholesterol. Consistently, berberine also upregulated LDL receptor (LDLR) mRNA level and suppressed HMGR gene expression. Meanwhile, upon berberine-treated rats, there was a significant increase in apolipoprotein E (apoE) mRNA, but no change in apoAI and scavenger receptor (SR) mRNA in the liver. Further, no atherosclerotic lesions were developed in berberine-treated rats for 16 weeks.

Conclusion: Berberine can counteract HFD-elicited hyperhomocysteinemia and hyperlipidemia partially via upregulating LDLR and apoE mRNA levels and suppressing HMGR gene expression.

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Related in: MedlinePlus

Effects of berberine (BBR) on body weight and food intake of rats. Male SD rats at 6 weeks of age received a high-fat diet (HFD) or regular rodent chow. After 8 weeks of feeding, rats were treated for 16 weeks with BBR or vehicle (n = 8 per group). A: The body weight is the average weight of 8 rats at the beginning of BBR treatment and after 16-week experiment. N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). B: Effects of BBR on food intake. The food intake was measured every week. Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.
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Figure 1: Effects of berberine (BBR) on body weight and food intake of rats. Male SD rats at 6 weeks of age received a high-fat diet (HFD) or regular rodent chow. After 8 weeks of feeding, rats were treated for 16 weeks with BBR or vehicle (n = 8 per group). A: The body weight is the average weight of 8 rats at the beginning of BBR treatment and after 16-week experiment. N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). B: Effects of BBR on food intake. The food intake was measured every week. Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.

Mentions: The body weight of SD rats was gradually increased with a high-fat diet for 24 weeks. Body weights of HFD-fed rats were significantly reduced in BBR -treated group for 16 weeks (Figure 1A) as compared with control group with no dramatic changes in food intake (Figure 1B).


The effects of berberine on hyperhomocysteinemia and hyperlipidemia in rats fed with a long-term high-fat diet.

Chang XX, Yan HM, Xu Q, Xia MF, Bian H, Zhu TF, Gao X - Lipids Health Dis (2012)

Effects of berberine (BBR) on body weight and food intake of rats. Male SD rats at 6 weeks of age received a high-fat diet (HFD) or regular rodent chow. After 8 weeks of feeding, rats were treated for 16 weeks with BBR or vehicle (n = 8 per group). A: The body weight is the average weight of 8 rats at the beginning of BBR treatment and after 16-week experiment. N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). B: Effects of BBR on food intake. The food intake was measured every week. Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475055&req=5

Figure 1: Effects of berberine (BBR) on body weight and food intake of rats. Male SD rats at 6 weeks of age received a high-fat diet (HFD) or regular rodent chow. After 8 weeks of feeding, rats were treated for 16 weeks with BBR or vehicle (n = 8 per group). A: The body weight is the average weight of 8 rats at the beginning of BBR treatment and after 16-week experiment. N, Normal control; C, vehicle –treated rats fed with HFD; B, rats with berberine treatment (200 mg/kg/d). B: Effects of BBR on food intake. The food intake was measured every week. Values are mean ± SEM. *p < 0.05vs N; #p < 0.05 vs C.
Mentions: The body weight of SD rats was gradually increased with a high-fat diet for 24 weeks. Body weights of HFD-fed rats were significantly reduced in BBR -treated group for 16 weeks (Figure 1A) as compared with control group with no dramatic changes in food intake (Figure 1B).

Bottom Line: The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.In parallel, it also decreased body weight and improved serum TC and LDL-c.Berberine also tended to decrease hepatic cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, China.

ABSTRACT

Background: The study was undertaken to examine the effects of berberine (BBR) on serum homocysteine, lipids and the aortic lesion in Sprague-Dawley (SD) rats fed with a long-term high-fat diet (HFD).

Methods: Healthy male SD rats weighing 190-210 g received randomly standard diet or a high-fat diet for 24 weeks. After 8 weeks of feeding, rats fed with HFD were randomized to receive berberine (200 mg · kg-1· day-1) or vehicle by gavage for 16 weeks. After overnight fasting, all rats were sacrificed and total blood samples were also collected for determinant of fasting serum homocysteine (Hcy), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels. The aorta was stained with hematoxylin and eosin (HE) and Sudan Ш to evaluate aortic lesion. The livers were dissected out and snap-frozen in liquid nitrogen for hepatic TC content and molecular analysis. 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), Lipoprotein receptors and apolipoproteins gene expression in the liver were determined by real-time PCR.

Results: Intragastrical administration with berberine for 16 weeks lowered serum Hcy in rats fed with a high-fat diet. In parallel, it also decreased body weight and improved serum TC and LDL-c. Berberine also tended to decrease hepatic cholesterol. Consistently, berberine also upregulated LDL receptor (LDLR) mRNA level and suppressed HMGR gene expression. Meanwhile, upon berberine-treated rats, there was a significant increase in apolipoprotein E (apoE) mRNA, but no change in apoAI and scavenger receptor (SR) mRNA in the liver. Further, no atherosclerotic lesions were developed in berberine-treated rats for 16 weeks.

Conclusion: Berberine can counteract HFD-elicited hyperhomocysteinemia and hyperlipidemia partially via upregulating LDLR and apoE mRNA levels and suppressing HMGR gene expression.

Show MeSH
Related in: MedlinePlus