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mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

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GABAergic synaptic transmission is low in the amygdala neurons of DBA mice. Spontaneous GABAergic inhibitory postsynaptic currents (IPSC) were recorded by whole-cell clamp at the amygdala neurons of brain slices from DBA/2 anxiety mice and control FVB/N mice. A) Left panels show sIPSCs recorded from FVB/N mice in top panel and sIPSCs with an extended time scale (bottom). Right panels show sIPSCs recorded from DBA/2 mice. B) shows cumulative probability vs. sIPSCs amplitudes from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. C) illustrates cumulative probability vs. intervals of events (sIPSCs) from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. D) shows cumulative probability vs. sIPSCs amplitudes in FVB/N mice (open symbols) and DBA/2 mice (filled) from all of the experiments (n = 12, p < 0.01). F) shows cumulative probability vs. intervals of events in FVB/N mice (open symbols) and DBA/2 mice (filled) averaged from all of the experiments (n = 11).
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Figure 6: GABAergic synaptic transmission is low in the amygdala neurons of DBA mice. Spontaneous GABAergic inhibitory postsynaptic currents (IPSC) were recorded by whole-cell clamp at the amygdala neurons of brain slices from DBA/2 anxiety mice and control FVB/N mice. A) Left panels show sIPSCs recorded from FVB/N mice in top panel and sIPSCs with an extended time scale (bottom). Right panels show sIPSCs recorded from DBA/2 mice. B) shows cumulative probability vs. sIPSCs amplitudes from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. C) illustrates cumulative probability vs. intervals of events (sIPSCs) from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. D) shows cumulative probability vs. sIPSCs amplitudes in FVB/N mice (open symbols) and DBA/2 mice (filled) from all of the experiments (n = 12, p < 0.01). F) shows cumulative probability vs. intervals of events in FVB/N mice (open symbols) and DBA/2 mice (filled) averaged from all of the experiments (n = 11).

Mentions: We also examined whether the weakness of GABAergic synaptic transmission was associated with the activity asynchrony of amygdala neurons in DBA/2 anxiety-like mice. Spontaneous inhibitory postsynaptic currents (IPSC) were recorded by whole- cell voltage-clamp at amygdala neurons of brain slices from DBA/2 and FVB/N mice. sIPSC events in Figure 6A were recorded in amygdala neurons from FVB/N mice (left panel) and DBA/2 mice (right). Cumulative probability versus sIPSC amplitudes and inter-event intervals analyzed from Figure 6A experiments in FVB/N (open symbols) and DBA/2 mice (filled) are presented in Figure 6B-C, respectively. Figure 6D illustrates cumulative probability versus sIPSC amplitudes averaged from all of the experiments in FVB/N (open symbols; n = 11) and DBA/2 mice (filled; n = 11). Figure 6E shows cumulative probability vs. inter-event intervals in FVB/N (open symbols) and DBA/2 (filled, n = 11, p < 0.01). Therefore, a weakness of GABAergic synaptic transmission at amygdala neurons is associated with DBA/2 anxiety-like mice.


mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

GABAergic synaptic transmission is low in the amygdala neurons of DBA mice. Spontaneous GABAergic inhibitory postsynaptic currents (IPSC) were recorded by whole-cell clamp at the amygdala neurons of brain slices from DBA/2 anxiety mice and control FVB/N mice. A) Left panels show sIPSCs recorded from FVB/N mice in top panel and sIPSCs with an extended time scale (bottom). Right panels show sIPSCs recorded from DBA/2 mice. B) shows cumulative probability vs. sIPSCs amplitudes from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. C) illustrates cumulative probability vs. intervals of events (sIPSCs) from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. D) shows cumulative probability vs. sIPSCs amplitudes in FVB/N mice (open symbols) and DBA/2 mice (filled) from all of the experiments (n = 12, p < 0.01). F) shows cumulative probability vs. intervals of events in FVB/N mice (open symbols) and DBA/2 mice (filled) averaged from all of the experiments (n = 11).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 6: GABAergic synaptic transmission is low in the amygdala neurons of DBA mice. Spontaneous GABAergic inhibitory postsynaptic currents (IPSC) were recorded by whole-cell clamp at the amygdala neurons of brain slices from DBA/2 anxiety mice and control FVB/N mice. A) Left panels show sIPSCs recorded from FVB/N mice in top panel and sIPSCs with an extended time scale (bottom). Right panels show sIPSCs recorded from DBA/2 mice. B) shows cumulative probability vs. sIPSCs amplitudes from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. C) illustrates cumulative probability vs. intervals of events (sIPSCs) from FVB/N mice (open symbols) and DBA/2 mice (filled ones) in an experiment of panel A. D) shows cumulative probability vs. sIPSCs amplitudes in FVB/N mice (open symbols) and DBA/2 mice (filled) from all of the experiments (n = 12, p < 0.01). F) shows cumulative probability vs. intervals of events in FVB/N mice (open symbols) and DBA/2 mice (filled) averaged from all of the experiments (n = 11).
Mentions: We also examined whether the weakness of GABAergic synaptic transmission was associated with the activity asynchrony of amygdala neurons in DBA/2 anxiety-like mice. Spontaneous inhibitory postsynaptic currents (IPSC) were recorded by whole- cell voltage-clamp at amygdala neurons of brain slices from DBA/2 and FVB/N mice. sIPSC events in Figure 6A were recorded in amygdala neurons from FVB/N mice (left panel) and DBA/2 mice (right). Cumulative probability versus sIPSC amplitudes and inter-event intervals analyzed from Figure 6A experiments in FVB/N (open symbols) and DBA/2 mice (filled) are presented in Figure 6B-C, respectively. Figure 6D illustrates cumulative probability versus sIPSC amplitudes averaged from all of the experiments in FVB/N (open symbols; n = 11) and DBA/2 mice (filled; n = 11). Figure 6E shows cumulative probability vs. inter-event intervals in FVB/N (open symbols) and DBA/2 (filled, n = 11, p < 0.01). Therefore, a weakness of GABAergic synaptic transmission at amygdala neurons is associated with DBA/2 anxiety-like mice.

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

Show MeSH
Related in: MedlinePlus