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mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

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The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the line scanning. A) shows the comparisons in the events of Ca2+ signals linearly scanned in FVB/N mice (left panel) and DBA/2 mice (right). B) shows the cross-correlations in the timing phase of activity between two neighboring neurons in FVB/N mice (top panel) and DBA/2 mice (bottom). Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. C) shows the comparisons in the cross-correlations averaged from visible amygdala neurons in all of FVB/N mice (red line, n = 6 for mice and n = 11 for slices) and DBA/2 ones (black, n = 5 for mice and n = 12 for slices; p < 0.05).
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Figure 4: The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the line scanning. A) shows the comparisons in the events of Ca2+ signals linearly scanned in FVB/N mice (left panel) and DBA/2 mice (right). B) shows the cross-correlations in the timing phase of activity between two neighboring neurons in FVB/N mice (top panel) and DBA/2 mice (bottom). Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. C) shows the comparisons in the cross-correlations averaged from visible amygdala neurons in all of FVB/N mice (red line, n = 6 for mice and n = 11 for slices) and DBA/2 ones (black, n = 5 for mice and n = 12 for slices; p < 0.05).

Mentions: We also conducted two-photon cellular imaging under the line scanning to examine the temporal activity of amygdala neurons in DBA/2 and FVB/N mice, in which the scanning rate reached above 50 Hz (Methods). Figure 4A shows the comparisons in the events of Ca2+ signals in FVB/N (left panel) and DBA/2 mice (right). The chip patterns in Figure 4B show cross-correlations in the timing phase of activity between two neighboring neurons in FVB/N (top panel) and DBA/2 mice (bottom). Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. Figure 4C compares the cross-correlations averaged from amygdala neurons in FVB/N mice (red line, n = 6 for mice and n = 11 for slices) and DBA/2 ones (black, n = 5 for mice and n = 12 for slices; p < 0.05). These data are consistent with those under the frame scanning, i.e., the activities among amygdala neurons are less synchronous in DBA/2 high anxiety mice than FVB/N low anxiety ones.


mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the line scanning. A) shows the comparisons in the events of Ca2+ signals linearly scanned in FVB/N mice (left panel) and DBA/2 mice (right). B) shows the cross-correlations in the timing phase of activity between two neighboring neurons in FVB/N mice (top panel) and DBA/2 mice (bottom). Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. C) shows the comparisons in the cross-correlations averaged from visible amygdala neurons in all of FVB/N mice (red line, n = 6 for mice and n = 11 for slices) and DBA/2 ones (black, n = 5 for mice and n = 12 for slices; p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475049&req=5

Figure 4: The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the line scanning. A) shows the comparisons in the events of Ca2+ signals linearly scanned in FVB/N mice (left panel) and DBA/2 mice (right). B) shows the cross-correlations in the timing phase of activity between two neighboring neurons in FVB/N mice (top panel) and DBA/2 mice (bottom). Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. C) shows the comparisons in the cross-correlations averaged from visible amygdala neurons in all of FVB/N mice (red line, n = 6 for mice and n = 11 for slices) and DBA/2 ones (black, n = 5 for mice and n = 12 for slices; p < 0.05).
Mentions: We also conducted two-photon cellular imaging under the line scanning to examine the temporal activity of amygdala neurons in DBA/2 and FVB/N mice, in which the scanning rate reached above 50 Hz (Methods). Figure 4A shows the comparisons in the events of Ca2+ signals in FVB/N (left panel) and DBA/2 mice (right). The chip patterns in Figure 4B show cross-correlations in the timing phase of activity between two neighboring neurons in FVB/N (top panel) and DBA/2 mice (bottom). Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. Figure 4C compares the cross-correlations averaged from amygdala neurons in FVB/N mice (red line, n = 6 for mice and n = 11 for slices) and DBA/2 ones (black, n = 5 for mice and n = 12 for slices; p < 0.05). These data are consistent with those under the frame scanning, i.e., the activities among amygdala neurons are less synchronous in DBA/2 high anxiety mice than FVB/N low anxiety ones.

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

Show MeSH
Related in: MedlinePlus