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mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

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The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the frame scanning. A) The picture of chip patterns shows the cross-correlations in the timing phase of activity between two neighboring neurons in DBA/2 mice. Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. B) shows the cross-correlations between neighboring neurons in FVB/N mice. C) shows a comparison in the cross-correlations averaged from all of the visible amygdala neurons in a FVB/N mouse (red line) and in a DBA/2 mouse (black), in which experiments were done in a single day (p < 0.01). D) illustrates a comparison in the cross-correlations averaged from all of FVB/N mice (red line; n = 11) and DBA/2 ones (black; p < 0.01, n = 12). E) shows a comparison in cross-correlations averaged from all of the astrocytes in FVB/N mice (red line) and DBA/2 ones (black; p = 0.56).
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Figure 3: The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the frame scanning. A) The picture of chip patterns shows the cross-correlations in the timing phase of activity between two neighboring neurons in DBA/2 mice. Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. B) shows the cross-correlations between neighboring neurons in FVB/N mice. C) shows a comparison in the cross-correlations averaged from all of the visible amygdala neurons in a FVB/N mouse (red line) and in a DBA/2 mouse (black), in which experiments were done in a single day (p < 0.01). D) illustrates a comparison in the cross-correlations averaged from all of FVB/N mice (red line; n = 11) and DBA/2 ones (black; p < 0.01, n = 12). E) shows a comparison in cross-correlations averaged from all of the astrocytes in FVB/N mice (red line) and DBA/2 ones (black; p = 0.56).

Mentions: Figures 2, 3 illustrate spatial and temporal patterns in the activity of amygdala network neurons from DBA/2 high anxiety and FVB/N low anxiety mice. Two-photon Ca2+ images in the neurons (green) and astrocytes (red/yellow) in amygdala slices from these mice are showed in Figure 2A-B, respectively. We analyzed their basal and spontaneous signals to present the activity strength of amygdala neurons, Figure 2C shows the number of cells versus their absolute fluorescence intensity (AFI) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black), in which average values are 1059 ± 581 for DBA/2 mice (n = 12 for mice and n = 27 for slices) and 1307 ± 676 for FVB/N mice (n = 11 for mice and n = 25 for slices). Figure 2D shows the number of spontaneous events versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black). The averaged values are 0.376 ± 0.14 for DBA/2 mice and 0.378 ± 0.11 for FVB/N mice (p = 0.96, n = 42). There is no difference in the activity strength of amygdala neurons between DBA/2 high anxiety and FVB/N low anxiety mice. Similarly, the activity strength of astrocytes in amygdala between DBA/2 and FVB/N mice is not difference (Figure 2E-F). We subsequently analyzed the temporal activity properties of nerve cells in amygdala.


mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the frame scanning. A) The picture of chip patterns shows the cross-correlations in the timing phase of activity between two neighboring neurons in DBA/2 mice. Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. B) shows the cross-correlations between neighboring neurons in FVB/N mice. C) shows a comparison in the cross-correlations averaged from all of the visible amygdala neurons in a FVB/N mouse (red line) and in a DBA/2 mouse (black), in which experiments were done in a single day (p < 0.01). D) illustrates a comparison in the cross-correlations averaged from all of FVB/N mice (red line; n = 11) and DBA/2 ones (black; p < 0.01, n = 12). E) shows a comparison in cross-correlations averaged from all of the astrocytes in FVB/N mice (red line) and DBA/2 ones (black; p = 0.56).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 3: The activities of amygdala neurons are less synchronous in DBA/2 mice than in FVB/N mice. Two-photon cellular imaging was conducted under the frame scanning. A) The picture of chip patterns shows the cross-correlations in the timing phase of activity between two neighboring neurons in DBA/2 mice. Colors from red to blue indicate their cross-correlations from high (synchronous activity) to low. B) shows the cross-correlations between neighboring neurons in FVB/N mice. C) shows a comparison in the cross-correlations averaged from all of the visible amygdala neurons in a FVB/N mouse (red line) and in a DBA/2 mouse (black), in which experiments were done in a single day (p < 0.01). D) illustrates a comparison in the cross-correlations averaged from all of FVB/N mice (red line; n = 11) and DBA/2 ones (black; p < 0.01, n = 12). E) shows a comparison in cross-correlations averaged from all of the astrocytes in FVB/N mice (red line) and DBA/2 ones (black; p = 0.56).
Mentions: Figures 2, 3 illustrate spatial and temporal patterns in the activity of amygdala network neurons from DBA/2 high anxiety and FVB/N low anxiety mice. Two-photon Ca2+ images in the neurons (green) and astrocytes (red/yellow) in amygdala slices from these mice are showed in Figure 2A-B, respectively. We analyzed their basal and spontaneous signals to present the activity strength of amygdala neurons, Figure 2C shows the number of cells versus their absolute fluorescence intensity (AFI) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black), in which average values are 1059 ± 581 for DBA/2 mice (n = 12 for mice and n = 27 for slices) and 1307 ± 676 for FVB/N mice (n = 11 for mice and n = 25 for slices). Figure 2D shows the number of spontaneous events versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black). The averaged values are 0.376 ± 0.14 for DBA/2 mice and 0.378 ± 0.11 for FVB/N mice (p = 0.96, n = 42). There is no difference in the activity strength of amygdala neurons between DBA/2 high anxiety and FVB/N low anxiety mice. Similarly, the activity strength of astrocytes in amygdala between DBA/2 and FVB/N mice is not difference (Figure 2E-F). We subsequently analyzed the temporal activity properties of nerve cells in amygdala.

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

Show MeSH
Related in: MedlinePlus