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mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

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The activity strength of amygdala neurons is not different in DBA/2 and FVB/N mice. Oregon -green BAPTA-AM was loaded into the cells in brain slices including amygdala to monitor Ca2+ levels in neurons and astrocytes. Sulforhodanmine-101 was used to label astrocytes. Fluorescents in amygdala areas were excited and detected by two-photon laser scanning microscopy. A) shows a photo of Ca2+ imaging from the neurons (green) and astrocytes (red and yellow) in DBA/2 mice. B) shows Ca2+ imaging from the neurons (green) and astrocytes (red/yellow) in FVB/N mice. C) shows the number of neurons vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). D) shows the number of spontaneous events from neurons versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve). E) shows the number of astrocytes vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). F) shows the number of spontaneous events from astrocytes versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve).
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Figure 2: The activity strength of amygdala neurons is not different in DBA/2 and FVB/N mice. Oregon -green BAPTA-AM was loaded into the cells in brain slices including amygdala to monitor Ca2+ levels in neurons and astrocytes. Sulforhodanmine-101 was used to label astrocytes. Fluorescents in amygdala areas were excited and detected by two-photon laser scanning microscopy. A) shows a photo of Ca2+ imaging from the neurons (green) and astrocytes (red and yellow) in DBA/2 mice. B) shows Ca2+ imaging from the neurons (green) and astrocytes (red/yellow) in FVB/N mice. C) shows the number of neurons vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). D) shows the number of spontaneous events from neurons versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve). E) shows the number of astrocytes vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). F) shows the number of spontaneous events from astrocytes versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve).

Mentions: Figures 2, 3 illustrate spatial and temporal patterns in the activity of amygdala network neurons from DBA/2 high anxiety and FVB/N low anxiety mice. Two-photon Ca2+ images in the neurons (green) and astrocytes (red/yellow) in amygdala slices from these mice are showed in Figure 2A-B, respectively. We analyzed their basal and spontaneous signals to present the activity strength of amygdala neurons, Figure 2C shows the number of cells versus their absolute fluorescence intensity (AFI) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black), in which average values are 1059 ± 581 for DBA/2 mice (n = 12 for mice and n = 27 for slices) and 1307 ± 676 for FVB/N mice (n = 11 for mice and n = 25 for slices). Figure 2D shows the number of spontaneous events versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black). The averaged values are 0.376 ± 0.14 for DBA/2 mice and 0.378 ± 0.11 for FVB/N mice (p = 0.96, n = 42). There is no difference in the activity strength of amygdala neurons between DBA/2 high anxiety and FVB/N low anxiety mice. Similarly, the activity strength of astrocytes in amygdala between DBA/2 and FVB/N mice is not difference (Figure 2E-F). We subsequently analyzed the temporal activity properties of nerve cells in amygdala.


mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

The activity strength of amygdala neurons is not different in DBA/2 and FVB/N mice. Oregon -green BAPTA-AM was loaded into the cells in brain slices including amygdala to monitor Ca2+ levels in neurons and astrocytes. Sulforhodanmine-101 was used to label astrocytes. Fluorescents in amygdala areas were excited and detected by two-photon laser scanning microscopy. A) shows a photo of Ca2+ imaging from the neurons (green) and astrocytes (red and yellow) in DBA/2 mice. B) shows Ca2+ imaging from the neurons (green) and astrocytes (red/yellow) in FVB/N mice. C) shows the number of neurons vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). D) shows the number of spontaneous events from neurons versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve). E) shows the number of astrocytes vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). F) shows the number of spontaneous events from astrocytes versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve).
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Figure 2: The activity strength of amygdala neurons is not different in DBA/2 and FVB/N mice. Oregon -green BAPTA-AM was loaded into the cells in brain slices including amygdala to monitor Ca2+ levels in neurons and astrocytes. Sulforhodanmine-101 was used to label astrocytes. Fluorescents in amygdala areas were excited and detected by two-photon laser scanning microscopy. A) shows a photo of Ca2+ imaging from the neurons (green) and astrocytes (red and yellow) in DBA/2 mice. B) shows Ca2+ imaging from the neurons (green) and astrocytes (red/yellow) in FVB/N mice. C) shows the number of neurons vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). D) shows the number of spontaneous events from neurons versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve). E) shows the number of astrocytes vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). F) shows the number of spontaneous events from astrocytes versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve).
Mentions: Figures 2, 3 illustrate spatial and temporal patterns in the activity of amygdala network neurons from DBA/2 high anxiety and FVB/N low anxiety mice. Two-photon Ca2+ images in the neurons (green) and astrocytes (red/yellow) in amygdala slices from these mice are showed in Figure 2A-B, respectively. We analyzed their basal and spontaneous signals to present the activity strength of amygdala neurons, Figure 2C shows the number of cells versus their absolute fluorescence intensity (AFI) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black), in which average values are 1059 ± 581 for DBA/2 mice (n = 12 for mice and n = 27 for slices) and 1307 ± 676 for FVB/N mice (n = 11 for mice and n = 25 for slices). Figure 2D shows the number of spontaneous events versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white/black). The averaged values are 0.376 ± 0.14 for DBA/2 mice and 0.378 ± 0.11 for FVB/N mice (p = 0.96, n = 42). There is no difference in the activity strength of amygdala neurons between DBA/2 high anxiety and FVB/N low anxiety mice. Similarly, the activity strength of astrocytes in amygdala between DBA/2 and FVB/N mice is not difference (Figure 2E-F). We subsequently analyzed the temporal activity properties of nerve cells in amygdala.

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

Show MeSH
Related in: MedlinePlus