Limits...
mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

Show MeSH

Related in: MedlinePlus

DBA/2 mice show higher anxiety compared to FVB/N mice. An elevated plus-maze was used to evaluate anxiety-like behavior in the strains of DBA/2 and FVB/N mice. A) The diagram shows the experimental methods to examine a mouse staying in the open arms vs. closed ones in an elevated plus-maze. B) shows the mouse position for counting exploration times. C) illustrates the duration for mice staying in the closed arms vs. total duration for each experiment in DBA/2 mice (gray bar) and FVB/N mice (white; p < 0.01, n = 12). D) shows exploration times towards the open arms for DBA/2 mice (gray bar) vs. FVB/N ones (white; p < 0.01, n = 12). The duration for staying in the closed arms is longer and the exploration times are lower significantly in DBA/2 mice than FVB/N mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3475049&req=5

Figure 1: DBA/2 mice show higher anxiety compared to FVB/N mice. An elevated plus-maze was used to evaluate anxiety-like behavior in the strains of DBA/2 and FVB/N mice. A) The diagram shows the experimental methods to examine a mouse staying in the open arms vs. closed ones in an elevated plus-maze. B) shows the mouse position for counting exploration times. C) illustrates the duration for mice staying in the closed arms vs. total duration for each experiment in DBA/2 mice (gray bar) and FVB/N mice (white; p < 0.01, n = 12). D) shows exploration times towards the open arms for DBA/2 mice (gray bar) vs. FVB/N ones (white; p < 0.01, n = 12). The duration for staying in the closed arms is longer and the exploration times are lower significantly in DBA/2 mice than FVB/N mice.

Mentions: Before used these mice, we first examined their anxiety-like behaviors with an elevated plus-maze [41,42]; and Figure 1A). High anxiety-like behavior is described as the mice spending more time in the closed arms and having less exploration times toward the open arms. In these measurements from DBA/2 and FVB/N mice, the duration of staying in the closed arms/total is longer in DBA/2 mice (gray bar) than FVB/N mice (white; p < 0.01, n = 12; Figure 1C). The exploration times toward the open arms are lower in DBA/2 mice (gray bar) than FVB/N ones (white; p < 0.01, n = 12; Figure 1D). The level of anxiety-like behaviors is higher in DBA/2 mice than FVB/N mice. This conclusion is consistent with a report about anxiety-like behaviors in different strains of mice [29].


mGluR₁,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice.

Zhang F, Liu B, Lei Z, Wang JH - Mol Brain (2012)

DBA/2 mice show higher anxiety compared to FVB/N mice. An elevated plus-maze was used to evaluate anxiety-like behavior in the strains of DBA/2 and FVB/N mice. A) The diagram shows the experimental methods to examine a mouse staying in the open arms vs. closed ones in an elevated plus-maze. B) shows the mouse position for counting exploration times. C) illustrates the duration for mice staying in the closed arms vs. total duration for each experiment in DBA/2 mice (gray bar) and FVB/N mice (white; p < 0.01, n = 12). D) shows exploration times towards the open arms for DBA/2 mice (gray bar) vs. FVB/N ones (white; p < 0.01, n = 12). The duration for staying in the closed arms is longer and the exploration times are lower significantly in DBA/2 mice than FVB/N mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475049&req=5

Figure 1: DBA/2 mice show higher anxiety compared to FVB/N mice. An elevated plus-maze was used to evaluate anxiety-like behavior in the strains of DBA/2 and FVB/N mice. A) The diagram shows the experimental methods to examine a mouse staying in the open arms vs. closed ones in an elevated plus-maze. B) shows the mouse position for counting exploration times. C) illustrates the duration for mice staying in the closed arms vs. total duration for each experiment in DBA/2 mice (gray bar) and FVB/N mice (white; p < 0.01, n = 12). D) shows exploration times towards the open arms for DBA/2 mice (gray bar) vs. FVB/N ones (white; p < 0.01, n = 12). The duration for staying in the closed arms is longer and the exploration times are lower significantly in DBA/2 mice than FVB/N mice.
Mentions: Before used these mice, we first examined their anxiety-like behaviors with an elevated plus-maze [41,42]; and Figure 1A). High anxiety-like behavior is described as the mice spending more time in the closed arms and having less exploration times toward the open arms. In these measurements from DBA/2 and FVB/N mice, the duration of staying in the closed arms/total is longer in DBA/2 mice (gray bar) than FVB/N mice (white; p < 0.01, n = 12; Figure 1C). The exploration times toward the open arms are lower in DBA/2 mice (gray bar) than FVB/N ones (white; p < 0.01, n = 12; Figure 1D). The level of anxiety-like behaviors is higher in DBA/2 mice than FVB/N mice. This conclusion is consistent with a report about anxiety-like behaviors in different strains of mice [29].

Bottom Line: Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved.Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects.The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.

ABSTRACT
Anxiety is a prevalent psychological disorder, in which the atypical expression of certain genes and the abnormality of amygdala are involved. Intermediate processes between genetic defects and anxiety, pathophysiological characteristics of neural network, remain unclear. Using behavioral task, two-photon cellular imaging and electrophysiology, we studied the characteristics of neural networks in basolateral amygdala and the influences of metabotropic glutamate receptor (mGluR) on their dynamics in DBA/2 mice showing anxiety-related genetic defects. Amygdala neurons in DBA/2 high anxiety mice express asynchronous activity and diverse excitability, and their GABAergic synapses demonstrate weak transmission, compared to those in low anxiety FVB/N mice. mGluR1,5 activation improves the anxiety-like behaviors of DBA/2 mice, synchronizes the activity of amygdala neurons and strengthens the transmission of GABAergic synapses. The activity asynchrony of amygdala neurons and the weakness of GABA synaptic transmission are associated with anxiety-like behavior.

Show MeSH
Related in: MedlinePlus