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Variation in the IL1B, TNF and IL6 genes and individual susceptibility to prosthetic joint infection.

Stahelova A, Mrazek F, Smizansky M, Petrek M, Gallo J - BMC Immunol. (2012)

Bottom Line: There was no significant difference in the distribution of the remaining five investigated CGPs and their haplotypes between groups.A functional variant of the gene encoding for IL-1beta was preliminarily nominated as a genetic factor contributing to the susceptibility to PJI.Our results should be independently replicated; studies on the functional relevance of IL1B gene variants in PJI are also needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Immunogenomics and Immunoproteomics, Faculty of Medicine and Dentistry, Palacky University Olomouc, IP Pavlova 6, Olomouc 77520, Czech Republic.

ABSTRACT

Background: Prosthetic joint infection (PJI) is an important failure mechanism of total joint arthroplasty (TJA). Here we examine whether the particular genetic variants can lead to increased susceptibility to PJI development.

Results: We conducted a genetic-association study to determine whether PJI could be associated with functional cytokine gene polymorphisms (CGP) influencing on innate immunity response. A case-control design was utilized and previously published criteria for PJI were included to distinguish between cases and control subjects with/without TJA. Six single nucleotide polymorphisms (SNPs) located in the genes for interleukin-1beta (SNP: IL1B-511, +3962), tumour necrosis factor alpha (TNF-308, -238) and interleukin-6 (IL6-174, nt565) were genotyped in 303 Caucasian (Czech) patients with TJA (89 with PJI / 214 without PJI), and 168 unrelated healthy Czech individuals without TJA. The results showed that carriers of the less common IL1B-511*T allele were overrepresented in the group of TJA patients with PJI (69%) in comparison with those that did not develop PJI (51%, p = 0.006, p(corr) = 0.037) and with healthy controls (55%, p = 0.04, p(corr) = N.S.). There was no significant difference in the distribution of the remaining five investigated CGPs and their haplotypes between groups.

Conclusion: A functional variant of the gene encoding for IL-1beta was preliminarily nominated as a genetic factor contributing to the susceptibility to PJI. Our results should be independently replicated; studies on the functional relevance of IL1B gene variants in PJI are also needed.

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Related in: MedlinePlus

Flowchart of the study design. TJA – Total Joint Arthroplasty; PJI – Prosthetic Joint Infection.
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Figure 1: Flowchart of the study design. TJA – Total Joint Arthroplasty; PJI – Prosthetic Joint Infection.

Mentions: In addition, 168 healthy people without TJA were recruited as a population control group [age, median (1st-3rd quartile): 28(24–34); males/females: 91/77]. All patients and controls were unrelated individuals of Czech Caucasian origin (Figure 1). The informed consent for the anonymous use of their DNA for the purposes of this study was obtained from all subjects. The study was performed with the approval of the local Ethics Committee.


Variation in the IL1B, TNF and IL6 genes and individual susceptibility to prosthetic joint infection.

Stahelova A, Mrazek F, Smizansky M, Petrek M, Gallo J - BMC Immunol. (2012)

Flowchart of the study design. TJA – Total Joint Arthroplasty; PJI – Prosthetic Joint Infection.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475038&req=5

Figure 1: Flowchart of the study design. TJA – Total Joint Arthroplasty; PJI – Prosthetic Joint Infection.
Mentions: In addition, 168 healthy people without TJA were recruited as a population control group [age, median (1st-3rd quartile): 28(24–34); males/females: 91/77]. All patients and controls were unrelated individuals of Czech Caucasian origin (Figure 1). The informed consent for the anonymous use of their DNA for the purposes of this study was obtained from all subjects. The study was performed with the approval of the local Ethics Committee.

Bottom Line: There was no significant difference in the distribution of the remaining five investigated CGPs and their haplotypes between groups.A functional variant of the gene encoding for IL-1beta was preliminarily nominated as a genetic factor contributing to the susceptibility to PJI.Our results should be independently replicated; studies on the functional relevance of IL1B gene variants in PJI are also needed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Immunogenomics and Immunoproteomics, Faculty of Medicine and Dentistry, Palacky University Olomouc, IP Pavlova 6, Olomouc 77520, Czech Republic.

ABSTRACT

Background: Prosthetic joint infection (PJI) is an important failure mechanism of total joint arthroplasty (TJA). Here we examine whether the particular genetic variants can lead to increased susceptibility to PJI development.

Results: We conducted a genetic-association study to determine whether PJI could be associated with functional cytokine gene polymorphisms (CGP) influencing on innate immunity response. A case-control design was utilized and previously published criteria for PJI were included to distinguish between cases and control subjects with/without TJA. Six single nucleotide polymorphisms (SNPs) located in the genes for interleukin-1beta (SNP: IL1B-511, +3962), tumour necrosis factor alpha (TNF-308, -238) and interleukin-6 (IL6-174, nt565) were genotyped in 303 Caucasian (Czech) patients with TJA (89 with PJI / 214 without PJI), and 168 unrelated healthy Czech individuals without TJA. The results showed that carriers of the less common IL1B-511*T allele were overrepresented in the group of TJA patients with PJI (69%) in comparison with those that did not develop PJI (51%, p = 0.006, p(corr) = 0.037) and with healthy controls (55%, p = 0.04, p(corr) = N.S.). There was no significant difference in the distribution of the remaining five investigated CGPs and their haplotypes between groups.

Conclusion: A functional variant of the gene encoding for IL-1beta was preliminarily nominated as a genetic factor contributing to the susceptibility to PJI. Our results should be independently replicated; studies on the functional relevance of IL1B gene variants in PJI are also needed.

Show MeSH
Related in: MedlinePlus