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Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests.

Kumar N, Singh JP, Pande V, Mishra N, Srivastava B, Kapoor R, Valecha N, Anvikar AR - Malar. J. (2012)

Bottom Line: PfHRP2 exhibited more polymorphism than PfHRP3.Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi, 110077, India.

ABSTRACT

Background: Rapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates.

Methods: One hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined.

Results: Extensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.

Conclusion: The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.

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Related in: MedlinePlus

Map of India showing geographical distribution of samples (140 P. falciparum isolates).1. Chhattisgarh (n = 40), 2. Gujarat (n = 20), 3. Karnataka (n = 10), 4. Madhya Pradesh (n = 20), 5. Orissa (n = 30), 6. West Bengal (n = 20).
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Figure 1: Map of India showing geographical distribution of samples (140 P. falciparum isolates).1. Chhattisgarh (n = 40), 2. Gujarat (n = 20), 3. Karnataka (n = 10), 4. Madhya Pradesh (n = 20), 5. Orissa (n = 30), 6. West Bengal (n = 20).

Mentions: Total 140 P. falciparum isolates were collected from six states (Chhattisgarh, West Bengal, Madhya Pradesh, Gujarat, Orissa and Karnataka) of India from December 2009 to September 2011 (Figure 1). These samples also included panels prepared for quality assurance of RDTs and culture adapted samples from the Malaria Parasite Bank of National Institute of Malaria Research (NIMR). The study was approved by institutional ethics committee of NIMR New Delhi, India. Written Informed consent was obtained from the participants. Consent was obtained from parents in case of minor. Blood samples were collected by finger prick and used for RDT testing. They were also stored as dried blood spots on filter papers. All the diagnosed cases of malaria were treated as per the national drug policy.


Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests.

Kumar N, Singh JP, Pande V, Mishra N, Srivastava B, Kapoor R, Valecha N, Anvikar AR - Malar. J. (2012)

Map of India showing geographical distribution of samples (140 P. falciparum isolates).1. Chhattisgarh (n = 40), 2. Gujarat (n = 20), 3. Karnataka (n = 10), 4. Madhya Pradesh (n = 20), 5. Orissa (n = 30), 6. West Bengal (n = 20).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3475030&req=5

Figure 1: Map of India showing geographical distribution of samples (140 P. falciparum isolates).1. Chhattisgarh (n = 40), 2. Gujarat (n = 20), 3. Karnataka (n = 10), 4. Madhya Pradesh (n = 20), 5. Orissa (n = 30), 6. West Bengal (n = 20).
Mentions: Total 140 P. falciparum isolates were collected from six states (Chhattisgarh, West Bengal, Madhya Pradesh, Gujarat, Orissa and Karnataka) of India from December 2009 to September 2011 (Figure 1). These samples also included panels prepared for quality assurance of RDTs and culture adapted samples from the Malaria Parasite Bank of National Institute of Malaria Research (NIMR). The study was approved by institutional ethics committee of NIMR New Delhi, India. Written Informed consent was obtained from the participants. Consent was obtained from parents in case of minor. Blood samples were collected by finger prick and used for RDT testing. They were also stored as dried blood spots on filter papers. All the diagnosed cases of malaria were treated as per the national drug policy.

Bottom Line: PfHRP2 exhibited more polymorphism than PfHRP3.Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi, 110077, India.

ABSTRACT

Background: Rapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates.

Methods: One hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined.

Results: Extensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.

Conclusion: The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.

Show MeSH
Related in: MedlinePlus