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Spontaneous generation of germline characteristics in mouse fibrosarcoma cells.

Ma Z, Hu Y, Jiang G, Hou J, Liu R, Lu Y, Liu C - Sci Rep (2012)

Bottom Line: In this study, we further showed that cells functioning as germline could be present in mouse fibrosarcoma cells (L929 cell line).Early germline-like cells spontaneously appeared in L929 cells and further differentiated into oocyte-like cells.These germline-like cells can, in turn, develop into blastocyst-like structures in vitro and cause teratocarcinomas in vivo, which is consistent with natural germ cells in function.

View Article: PubMed Central - PubMed

Affiliation: Department of Labratory Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China.

ABSTRACT
Germline/embryonic-specific genes have been found to be activated in somatic tumors. In this study, we further showed that cells functioning as germline could be present in mouse fibrosarcoma cells (L929 cell line). Early germline-like cells spontaneously appeared in L929 cells and further differentiated into oocyte-like cells. These germline-like cells can, in turn, develop into blastocyst-like structures in vitro and cause teratocarcinomas in vivo, which is consistent with natural germ cells in function. Generation of germline-like cells from somatic tumors might provide a novel way to understand why somatic cancer cells have strong features of embryonic/germline development. It is thought that the germline traits of tumors are associated with the central characteristics of malignancy, such as immortalization, invasion, migration and immune evasion. Therefore, germline-like cells in tumors might provide potential targets to tumor biology, diagnosis and therapy.

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Pathological features and marker expression of tumors derived from L929 cells with germline-like cells.(a-d) Pathological features of the poor-differentiated section and germline relative markers. (a) Pathological features. (b) Oct4. (c) DAZL. (d) VASA. (e-i) Pathological features of sarcoma-like section and expression of germline relative markers. (e) Pathological features. (f) Oct4. (g) DAZL. (h) VASA. (i) SCP3. (j) showing epithelium-like and sarcoma-like pathological features. (k-o) showing the well-differentiated phenotypes in the tumors. (k) Striated muscles. (l) Bone-like tissues. (m) Gland-like tissues. (n) Digestive gland-like tissues. (o) Liver-like tissues. (p) AFP expression in (o). (q) Tumor growth curve from germline-like cells and adherent L929 cultures at day 3. Scale bars = 50 μm in (a-e, l, m ), 25 μm in (f-k, n-p).
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f5: Pathological features and marker expression of tumors derived from L929 cells with germline-like cells.(a-d) Pathological features of the poor-differentiated section and germline relative markers. (a) Pathological features. (b) Oct4. (c) DAZL. (d) VASA. (e-i) Pathological features of sarcoma-like section and expression of germline relative markers. (e) Pathological features. (f) Oct4. (g) DAZL. (h) VASA. (i) SCP3. (j) showing epithelium-like and sarcoma-like pathological features. (k-o) showing the well-differentiated phenotypes in the tumors. (k) Striated muscles. (l) Bone-like tissues. (m) Gland-like tissues. (n) Digestive gland-like tissues. (o) Liver-like tissues. (p) AFP expression in (o). (q) Tumor growth curve from germline-like cells and adherent L929 cultures at day 3. Scale bars = 50 μm in (a-e, l, m ), 25 μm in (f-k, n-p).

Mentions: Histological examinations were conducted to analyze the tumor types derived from the germline-like cells in vivo. Various differentiated tissues and minimally differentiated tissues from different layers could be observed in the tumors (Figure 5 a–p), including low-differentiation (Figure 5a), sarcoma-like components (Figure 5e, minimally differentiated), epithelial tissues (Figure 5j, minimally differentiated), striated muscles (Figure 5k, mesoderm), bone tissues (Figure 5l, mesoderm), gland-like epithelial tissues (Figure 5m, endoderm), digestive gland-like tissues (Figure 5n, endoderm) and liver-like tissues (Figure 5o, endoderm). However, neural tissues (ectoderm) were not observed in the tumors. The Oct4 (Figure 5d) was detected but DAZL (Figure 5c), Vasa (Figure 5 d), and SCP3 (not shown) were undetected in the low-differentiation section of the tumors. The Oct4 (Figure 5f), DAZL (Figure 5g), and Vasa (Figure 5h) were detected while SCP3 (Figure 5i) were undetected in the sarcoma-like section of the tumors, indicating that germline-like cells might be present in the tumors. Therefore, these results showed that the germline-like cells could give rise to teratocarcinomas in vivo, which indicates the germline-like cells similar to natural germ cells in their ability to form tumors.


Spontaneous generation of germline characteristics in mouse fibrosarcoma cells.

Ma Z, Hu Y, Jiang G, Hou J, Liu R, Lu Y, Liu C - Sci Rep (2012)

Pathological features and marker expression of tumors derived from L929 cells with germline-like cells.(a-d) Pathological features of the poor-differentiated section and germline relative markers. (a) Pathological features. (b) Oct4. (c) DAZL. (d) VASA. (e-i) Pathological features of sarcoma-like section and expression of germline relative markers. (e) Pathological features. (f) Oct4. (g) DAZL. (h) VASA. (i) SCP3. (j) showing epithelium-like and sarcoma-like pathological features. (k-o) showing the well-differentiated phenotypes in the tumors. (k) Striated muscles. (l) Bone-like tissues. (m) Gland-like tissues. (n) Digestive gland-like tissues. (o) Liver-like tissues. (p) AFP expression in (o). (q) Tumor growth curve from germline-like cells and adherent L929 cultures at day 3. Scale bars = 50 μm in (a-e, l, m ), 25 μm in (f-k, n-p).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3473365&req=5

f5: Pathological features and marker expression of tumors derived from L929 cells with germline-like cells.(a-d) Pathological features of the poor-differentiated section and germline relative markers. (a) Pathological features. (b) Oct4. (c) DAZL. (d) VASA. (e-i) Pathological features of sarcoma-like section and expression of germline relative markers. (e) Pathological features. (f) Oct4. (g) DAZL. (h) VASA. (i) SCP3. (j) showing epithelium-like and sarcoma-like pathological features. (k-o) showing the well-differentiated phenotypes in the tumors. (k) Striated muscles. (l) Bone-like tissues. (m) Gland-like tissues. (n) Digestive gland-like tissues. (o) Liver-like tissues. (p) AFP expression in (o). (q) Tumor growth curve from germline-like cells and adherent L929 cultures at day 3. Scale bars = 50 μm in (a-e, l, m ), 25 μm in (f-k, n-p).
Mentions: Histological examinations were conducted to analyze the tumor types derived from the germline-like cells in vivo. Various differentiated tissues and minimally differentiated tissues from different layers could be observed in the tumors (Figure 5 a–p), including low-differentiation (Figure 5a), sarcoma-like components (Figure 5e, minimally differentiated), epithelial tissues (Figure 5j, minimally differentiated), striated muscles (Figure 5k, mesoderm), bone tissues (Figure 5l, mesoderm), gland-like epithelial tissues (Figure 5m, endoderm), digestive gland-like tissues (Figure 5n, endoderm) and liver-like tissues (Figure 5o, endoderm). However, neural tissues (ectoderm) were not observed in the tumors. The Oct4 (Figure 5d) was detected but DAZL (Figure 5c), Vasa (Figure 5 d), and SCP3 (not shown) were undetected in the low-differentiation section of the tumors. The Oct4 (Figure 5f), DAZL (Figure 5g), and Vasa (Figure 5h) were detected while SCP3 (Figure 5i) were undetected in the sarcoma-like section of the tumors, indicating that germline-like cells might be present in the tumors. Therefore, these results showed that the germline-like cells could give rise to teratocarcinomas in vivo, which indicates the germline-like cells similar to natural germ cells in their ability to form tumors.

Bottom Line: In this study, we further showed that cells functioning as germline could be present in mouse fibrosarcoma cells (L929 cell line).Early germline-like cells spontaneously appeared in L929 cells and further differentiated into oocyte-like cells.These germline-like cells can, in turn, develop into blastocyst-like structures in vitro and cause teratocarcinomas in vivo, which is consistent with natural germ cells in function.

View Article: PubMed Central - PubMed

Affiliation: Department of Labratory Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China.

ABSTRACT
Germline/embryonic-specific genes have been found to be activated in somatic tumors. In this study, we further showed that cells functioning as germline could be present in mouse fibrosarcoma cells (L929 cell line). Early germline-like cells spontaneously appeared in L929 cells and further differentiated into oocyte-like cells. These germline-like cells can, in turn, develop into blastocyst-like structures in vitro and cause teratocarcinomas in vivo, which is consistent with natural germ cells in function. Generation of germline-like cells from somatic tumors might provide a novel way to understand why somatic cancer cells have strong features of embryonic/germline development. It is thought that the germline traits of tumors are associated with the central characteristics of malignancy, such as immortalization, invasion, migration and immune evasion. Therefore, germline-like cells in tumors might provide potential targets to tumor biology, diagnosis and therapy.

Show MeSH
Related in: MedlinePlus