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Congruence and indifference between two molecular markers for understanding oral evolution in the Marynidae sensu lato (Ciliophora, Colpodea).

Dunthorn M, Katz LA, Stoeck T, Foissner W - Eur. J. Protistol. (2012)

Bottom Line: Our understanding of the evolution of oral structures within the Colpodida is confounded by the low number of morphological characters that can be used in constructing hypotheses, and by the low taxon and character sampling in molecular phylogenetic analyses designed to assess these hypotheses.We show that the inferred mitochondrial and nuclear SSU-rDNA trees, as well as concatenated and constrained analyses, are congruent in not recovering a monophyletic Marynidae.However, due to low node support, the trees are indifferent to whether the morphological characters used to unite the Marynidae are the result of retention of ancestral states or convergence.

View Article: PubMed Central - PubMed

Affiliation: Department of Ecology, University of Kaiserslautern, Germany. dunthorn@rhrk.uni-kl.de

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Nuclear SSU-rDNA topology of the Colpodida. The most likely ML tree and its branch lengths are shown. The Bayesian tree inferred using MrBayes and the ML tree are identical in topology for moderately to highly supported nodes. Node support is as follows: ML bootstrap/BI posterior probability. Support <50% is shown as ‘–’.
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fig0010: Nuclear SSU-rDNA topology of the Colpodida. The most likely ML tree and its branch lengths are shown. The Bayesian tree inferred using MrBayes and the ML tree are identical in topology for moderately to highly supported nodes. Node support is as follows: ML bootstrap/BI posterior probability. Support <50% is shown as ‘–’.

Mentions: To evaluate the possibility of low taxon sampling affecting the inferred tree, taxon inclusion for nSSU-rDNA was limited to match the alignment for mtSSU-rDNA. This nuclear alignment of 1676 included characters resulted in identical ML and Bayesian topologies for moderately to highly supported nodes (Fig. 2). For non-Marynidae sequences, the nSSU-rDNA topology is congruent with previously published trees (Bourland et al. 2011; Dunthorn et al. 2008, 2009, 2011; Foissner et al. 2011; Quintela-Alonso et al. 2011) for moderately to highly supported nodes. Thus, the limited taxon inclusion does not appear to have an effect. As in the mtSSU-rDNA tree, the Marynidae s.l. are not monophyletic. Ilsiella branches sister to all Colpodida, except Bardeliella. The two Maryna sequences, which are sister to each other with full node support (100/100), form a clade with Hausmanniella and Colpoda aspera, although the node for this larger clade is not supported (<50/71). As the intervening nodes between Maryna and Ilsiella are not moderately to fully supported, the nSSU-rDNA tree provides little confidence in the non-monophyly of the Marynidae.


Congruence and indifference between two molecular markers for understanding oral evolution in the Marynidae sensu lato (Ciliophora, Colpodea).

Dunthorn M, Katz LA, Stoeck T, Foissner W - Eur. J. Protistol. (2012)

Nuclear SSU-rDNA topology of the Colpodida. The most likely ML tree and its branch lengths are shown. The Bayesian tree inferred using MrBayes and the ML tree are identical in topology for moderately to highly supported nodes. Node support is as follows: ML bootstrap/BI posterior probability. Support <50% is shown as ‘–’.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3473355&req=5

fig0010: Nuclear SSU-rDNA topology of the Colpodida. The most likely ML tree and its branch lengths are shown. The Bayesian tree inferred using MrBayes and the ML tree are identical in topology for moderately to highly supported nodes. Node support is as follows: ML bootstrap/BI posterior probability. Support <50% is shown as ‘–’.
Mentions: To evaluate the possibility of low taxon sampling affecting the inferred tree, taxon inclusion for nSSU-rDNA was limited to match the alignment for mtSSU-rDNA. This nuclear alignment of 1676 included characters resulted in identical ML and Bayesian topologies for moderately to highly supported nodes (Fig. 2). For non-Marynidae sequences, the nSSU-rDNA topology is congruent with previously published trees (Bourland et al. 2011; Dunthorn et al. 2008, 2009, 2011; Foissner et al. 2011; Quintela-Alonso et al. 2011) for moderately to highly supported nodes. Thus, the limited taxon inclusion does not appear to have an effect. As in the mtSSU-rDNA tree, the Marynidae s.l. are not monophyletic. Ilsiella branches sister to all Colpodida, except Bardeliella. The two Maryna sequences, which are sister to each other with full node support (100/100), form a clade with Hausmanniella and Colpoda aspera, although the node for this larger clade is not supported (<50/71). As the intervening nodes between Maryna and Ilsiella are not moderately to fully supported, the nSSU-rDNA tree provides little confidence in the non-monophyly of the Marynidae.

Bottom Line: Our understanding of the evolution of oral structures within the Colpodida is confounded by the low number of morphological characters that can be used in constructing hypotheses, and by the low taxon and character sampling in molecular phylogenetic analyses designed to assess these hypotheses.We show that the inferred mitochondrial and nuclear SSU-rDNA trees, as well as concatenated and constrained analyses, are congruent in not recovering a monophyletic Marynidae.However, due to low node support, the trees are indifferent to whether the morphological characters used to unite the Marynidae are the result of retention of ancestral states or convergence.

View Article: PubMed Central - PubMed

Affiliation: Department of Ecology, University of Kaiserslautern, Germany. dunthorn@rhrk.uni-kl.de

Show MeSH
Related in: MedlinePlus