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Skeletal muscle alterations and exercise performance decrease in erythropoietin-deficient mice: a comparative study.

Mille-Hamard L, Billat VL, Henry E, Bonnamy B, Joly F, Benech P, Barrey E - BMC Med Genomics (2012)

Bottom Line: Our results showed that the lack of functional EPO induced a decrease in the aerobic exercise capacity.The observed alterations in the muscle transcriptome suggest that physiological concentrations of EPO exert both direct and indirect muscle-protecting effects during exercise.However, the signaling pathway involved in these protective effects remains to be described in detail.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unité de Biologie Intégrative des Adaptations à l'Exercice - INSERM 902, Genopole, F-91058, Evry, France. laurence.hamard@inserm.fr

ABSTRACT

Background: Erythropoietin (EPO) is known to improve exercise performance by increasing oxygen blood transport and thus inducing a higher maximum oxygen uptake (VO2max). Furthermore, treatment with (or overexpression of) EPO induces protective effects in several tissues, including the myocardium. However, it is not known whether EPO exerts this protective effect when present at physiological levels. Given that EPO receptors have been identified in skeletal muscle, we hypothesized that EPO may have a direct, protective effect on this tissue. Thus, the objectives of the present study were to confirm a decrease in exercise performance and highlight muscle transcriptome alterations in a murine EPO functional knock-out model (the EPO-d mouse).

Methods: We determined VO2max peak velocity and critical speed in exhaustive runs in 17 mice (9 EPO-d animals and 8 inbred controls), using treadmill enclosed in a metabolic chamber. Mice were sacrificed 24h after a last exhaustive treadmill exercise at critical speed. The tibialis anterior and soleus muscles were removed and total RNA was extracted for microarray gene expression analysis.

Results: The EPO-d mice's hematocrit was about 50% lower than that of controls (p<0.05) and their performance level was about 25% lower (p<0.001). A total of 1583 genes exhibited significant changes in their expression levels. However, 68 genes were strongly up-regulated (normalized ratio>1.4) and 115 were strongly down-regulated (normalized ratio<0.80). The transcriptome data mining analysis showed that the exercise in the EPO-d mice induced muscle hypoxia, oxidative stress and proteolysis associated with energy pathway disruptions in glycolysis and mitochondrial oxidative phosphorylation.

Conclusions: Our results showed that the lack of functional EPO induced a decrease in the aerobic exercise capacity. This decrease was correlated with the hematocrit and reflecting poor oxygen supply to the muscles. The observed alterations in the muscle transcriptome suggest that physiological concentrations of EPO exert both direct and indirect muscle-protecting effects during exercise. However, the signaling pathway involved in these protective effects remains to be described in detail.

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Down-regulation of many genes involved in intracellular trafficking. Legends of this figure are provided in Figure 2.
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Figure 5: Down-regulation of many genes involved in intracellular trafficking. Legends of this figure are provided in Figure 2.

Mentions: The tissue, organ and cellular context and the associated biological activities were further investigated with a Predictsearch® analysis. Using the PubMed database, it was found that 31 of the significantly modulated genes were strongly associated with the following biological activities: apoptosis, proteolysis, glycolysis, cellular trafficking and cytoskeleton. In addition, the most frequently cited disease pathways in relation to the gene set were muscle atrophy, hypoxia and oxidative stress. The main pathway disruptions revealed by Predictsearch® data mining are described in Figures 2,3,4 and5.


Skeletal muscle alterations and exercise performance decrease in erythropoietin-deficient mice: a comparative study.

Mille-Hamard L, Billat VL, Henry E, Bonnamy B, Joly F, Benech P, Barrey E - BMC Med Genomics (2012)

Down-regulation of many genes involved in intracellular trafficking. Legends of this figure are provided in Figure 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3473259&req=5

Figure 5: Down-regulation of many genes involved in intracellular trafficking. Legends of this figure are provided in Figure 2.
Mentions: The tissue, organ and cellular context and the associated biological activities were further investigated with a Predictsearch® analysis. Using the PubMed database, it was found that 31 of the significantly modulated genes were strongly associated with the following biological activities: apoptosis, proteolysis, glycolysis, cellular trafficking and cytoskeleton. In addition, the most frequently cited disease pathways in relation to the gene set were muscle atrophy, hypoxia and oxidative stress. The main pathway disruptions revealed by Predictsearch® data mining are described in Figures 2,3,4 and5.

Bottom Line: Our results showed that the lack of functional EPO induced a decrease in the aerobic exercise capacity.The observed alterations in the muscle transcriptome suggest that physiological concentrations of EPO exert both direct and indirect muscle-protecting effects during exercise.However, the signaling pathway involved in these protective effects remains to be described in detail.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unité de Biologie Intégrative des Adaptations à l'Exercice - INSERM 902, Genopole, F-91058, Evry, France. laurence.hamard@inserm.fr

ABSTRACT

Background: Erythropoietin (EPO) is known to improve exercise performance by increasing oxygen blood transport and thus inducing a higher maximum oxygen uptake (VO2max). Furthermore, treatment with (or overexpression of) EPO induces protective effects in several tissues, including the myocardium. However, it is not known whether EPO exerts this protective effect when present at physiological levels. Given that EPO receptors have been identified in skeletal muscle, we hypothesized that EPO may have a direct, protective effect on this tissue. Thus, the objectives of the present study were to confirm a decrease in exercise performance and highlight muscle transcriptome alterations in a murine EPO functional knock-out model (the EPO-d mouse).

Methods: We determined VO2max peak velocity and critical speed in exhaustive runs in 17 mice (9 EPO-d animals and 8 inbred controls), using treadmill enclosed in a metabolic chamber. Mice were sacrificed 24h after a last exhaustive treadmill exercise at critical speed. The tibialis anterior and soleus muscles were removed and total RNA was extracted for microarray gene expression analysis.

Results: The EPO-d mice's hematocrit was about 50% lower than that of controls (p<0.05) and their performance level was about 25% lower (p<0.001). A total of 1583 genes exhibited significant changes in their expression levels. However, 68 genes were strongly up-regulated (normalized ratio>1.4) and 115 were strongly down-regulated (normalized ratio<0.80). The transcriptome data mining analysis showed that the exercise in the EPO-d mice induced muscle hypoxia, oxidative stress and proteolysis associated with energy pathway disruptions in glycolysis and mitochondrial oxidative phosphorylation.

Conclusions: Our results showed that the lack of functional EPO induced a decrease in the aerobic exercise capacity. This decrease was correlated with the hematocrit and reflecting poor oxygen supply to the muscles. The observed alterations in the muscle transcriptome suggest that physiological concentrations of EPO exert both direct and indirect muscle-protecting effects during exercise. However, the signaling pathway involved in these protective effects remains to be described in detail.

Show MeSH
Related in: MedlinePlus