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Immunoreactivities of androgen receptor, estrogen receptors, p450arom, p450c17 proteins in wild ground squirrels ovaries during the nonbreeding and breeding seasons.

Li X, Zhang H, Sheng X, Li B, Zhou J, Xu M, Weng Q, Watanabe G, Taya K - J Ovarian Res (2012)

Bottom Line: However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season.The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons.In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China. qiangweng@bjfu.edu.cn.

ABSTRACT
The aim of this study was to elucidate the regulatory role of androgen in the follicular development of wild female ground squirrels. Immunohistochemical staining of FSHR, LHR, P450c17, P450arom, androgen receptor (AR), estrogen receptors (ERa and ERb) were executed in ovaries of female ground squirrels from both breeding and nonbreeding seasons. In addition, total ovarian proteins were extracted from the ovaries of squirrels from breeding and nonbreeding seasons, and Western blot analysis were performed in order to probe for FSHR, LHR, P450c17, P450arom, AR, ERa and ERb. The results of immunohistochemical staining and Western blotting of P450c17 showed that there was no significant difference between the breeding and nonbreeding seasons. It was found that granulosa cells expressed P450arom during the breeding season. In contrast, there was no positive staining of P450arom in the nonbreeding season. There was no significant difference in immunoreactivity of AR between the breeding and nonbreeding seasons. However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season. The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons. In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season. These findings suggested that androgen might be predominantly converted into estrogen in order to regulate the follicular development via binding of estrogen receptors during the breeding season, whereas androgen might predominantly directly bind androgen receptor to regulate the follicular development during the nonbreeding season in the ovaries of wild female ground squirrels.

No MeSH data available.


The ratio of AR to ERs in the ovary of wild ground squirrels. The ratio of AR to ERa (a) and ERb (b) in the ovary of wild ground squirrels according to the expression level of AR and ERs. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001.
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Figure 6: The ratio of AR to ERs in the ovary of wild ground squirrels. The ratio of AR to ERa (a) and ERb (b) in the ovary of wild ground squirrels according to the expression level of AR and ERs. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001.

Mentions: The results of Western blotting analysis for FSHR, LHR, P450c17, P450arom, AR and ERs in the ovaries of the breeding and nonbreeding seasons were shown in Figure 5. The proteins extract from the ovaries of the breeding and nonbreeding seasons were loaded in the lane 1 and lane 2, respectively. In addition, b-actin was used as the endogenous control. Meanwhile, the expression levels of FSHR, LHR, P450c17, P450arom, AR, ERa and ERb were analyzed according to the optical density, which were shown in Figure 5 a’-g’ respectively. There was no significant change for the expression of P450c17 between the breeding and nonbreeding seasons (Figure 5c’). However, the expression of P450arom reduced significantly from the breeding to nonbreeding season (Figure 5d’). Similar to the expression pattern of P450c17, the immunoreactivity of AR was not significantly different in the breeding season versus the nonbreeding season (Figure 5e’). Moreover, the immunoreactivities of ERa and ERb were both remarkably reduced from the breeding to nonbreeding season (Figure 5 f’ and g’, respectively). Meanwhile, the immunoreactivities of FSHR and LHR decreased observably in the ovaries of the nonbreeding season when compared with the immunoreactivities of FSHR and LHR in the ovaries of the breeding season (Figure 5 a’ and b’, respectively). In addition, the ratio of AR to ERs was shown in Figure 6. Both the ratio of AR to ERa and the ratio of AR to ERb were increased significantly from the breeding to nonbreeding season in the ovaries of wild female ground squirrels.


Immunoreactivities of androgen receptor, estrogen receptors, p450arom, p450c17 proteins in wild ground squirrels ovaries during the nonbreeding and breeding seasons.

Li X, Zhang H, Sheng X, Li B, Zhou J, Xu M, Weng Q, Watanabe G, Taya K - J Ovarian Res (2012)

The ratio of AR to ERs in the ovary of wild ground squirrels. The ratio of AR to ERa (a) and ERb (b) in the ovary of wild ground squirrels according to the expression level of AR and ERs. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3473255&req=5

Figure 6: The ratio of AR to ERs in the ovary of wild ground squirrels. The ratio of AR to ERa (a) and ERb (b) in the ovary of wild ground squirrels according to the expression level of AR and ERs. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001.
Mentions: The results of Western blotting analysis for FSHR, LHR, P450c17, P450arom, AR and ERs in the ovaries of the breeding and nonbreeding seasons were shown in Figure 5. The proteins extract from the ovaries of the breeding and nonbreeding seasons were loaded in the lane 1 and lane 2, respectively. In addition, b-actin was used as the endogenous control. Meanwhile, the expression levels of FSHR, LHR, P450c17, P450arom, AR, ERa and ERb were analyzed according to the optical density, which were shown in Figure 5 a’-g’ respectively. There was no significant change for the expression of P450c17 between the breeding and nonbreeding seasons (Figure 5c’). However, the expression of P450arom reduced significantly from the breeding to nonbreeding season (Figure 5d’). Similar to the expression pattern of P450c17, the immunoreactivity of AR was not significantly different in the breeding season versus the nonbreeding season (Figure 5e’). Moreover, the immunoreactivities of ERa and ERb were both remarkably reduced from the breeding to nonbreeding season (Figure 5 f’ and g’, respectively). Meanwhile, the immunoreactivities of FSHR and LHR decreased observably in the ovaries of the nonbreeding season when compared with the immunoreactivities of FSHR and LHR in the ovaries of the breeding season (Figure 5 a’ and b’, respectively). In addition, the ratio of AR to ERs was shown in Figure 6. Both the ratio of AR to ERa and the ratio of AR to ERb were increased significantly from the breeding to nonbreeding season in the ovaries of wild female ground squirrels.

Bottom Line: However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season.The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons.In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China. qiangweng@bjfu.edu.cn.

ABSTRACT
The aim of this study was to elucidate the regulatory role of androgen in the follicular development of wild female ground squirrels. Immunohistochemical staining of FSHR, LHR, P450c17, P450arom, androgen receptor (AR), estrogen receptors (ERa and ERb) were executed in ovaries of female ground squirrels from both breeding and nonbreeding seasons. In addition, total ovarian proteins were extracted from the ovaries of squirrels from breeding and nonbreeding seasons, and Western blot analysis were performed in order to probe for FSHR, LHR, P450c17, P450arom, AR, ERa and ERb. The results of immunohistochemical staining and Western blotting of P450c17 showed that there was no significant difference between the breeding and nonbreeding seasons. It was found that granulosa cells expressed P450arom during the breeding season. In contrast, there was no positive staining of P450arom in the nonbreeding season. There was no significant difference in immunoreactivity of AR between the breeding and nonbreeding seasons. However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season. The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons. In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season. These findings suggested that androgen might be predominantly converted into estrogen in order to regulate the follicular development via binding of estrogen receptors during the breeding season, whereas androgen might predominantly directly bind androgen receptor to regulate the follicular development during the nonbreeding season in the ovaries of wild female ground squirrels.

No MeSH data available.