Limits...
Immunoreactivities of androgen receptor, estrogen receptors, p450arom, p450c17 proteins in wild ground squirrels ovaries during the nonbreeding and breeding seasons.

Li X, Zhang H, Sheng X, Li B, Zhou J, Xu M, Weng Q, Watanabe G, Taya K - J Ovarian Res (2012)

Bottom Line: However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season.The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons.In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China. qiangweng@bjfu.edu.cn.

ABSTRACT
The aim of this study was to elucidate the regulatory role of androgen in the follicular development of wild female ground squirrels. Immunohistochemical staining of FSHR, LHR, P450c17, P450arom, androgen receptor (AR), estrogen receptors (ERa and ERb) were executed in ovaries of female ground squirrels from both breeding and nonbreeding seasons. In addition, total ovarian proteins were extracted from the ovaries of squirrels from breeding and nonbreeding seasons, and Western blot analysis were performed in order to probe for FSHR, LHR, P450c17, P450arom, AR, ERa and ERb. The results of immunohistochemical staining and Western blotting of P450c17 showed that there was no significant difference between the breeding and nonbreeding seasons. It was found that granulosa cells expressed P450arom during the breeding season. In contrast, there was no positive staining of P450arom in the nonbreeding season. There was no significant difference in immunoreactivity of AR between the breeding and nonbreeding seasons. However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season. The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons. In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season. These findings suggested that androgen might be predominantly converted into estrogen in order to regulate the follicular development via binding of estrogen receptors during the breeding season, whereas androgen might predominantly directly bind androgen receptor to regulate the follicular development during the nonbreeding season in the ovaries of wild female ground squirrels.

No MeSH data available.


Related in: MedlinePlus

Seasonal changes in the ovary of wild female ground squirrels.a: The histological observation and diagram of the ovary in breeding season. b: The histological observation and diagram of the ovary in nonbreeding season. c: Histological ovarian pattern diagram of the wild ground squirrels during the breeding season. d: Histological ovarian pattern diagram of the wild ground squirrels during the nonbreeding season. e: Seasonal change of the number of primary follicles. f: Seasonal change of the number of secondary follicles. g: Seasonal change of the number of antral, post-antral follicles. h: Seasonal change of the number of corpus luteum. PF, Primary Follicle; SF, Secondary Follicle; AF, Antral Follicle; DF, Dominant Follicle; CL, Corpus Luteum. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001. Bars = 200μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3473255&req=5

Figure 1: Seasonal changes in the ovary of wild female ground squirrels.a: The histological observation and diagram of the ovary in breeding season. b: The histological observation and diagram of the ovary in nonbreeding season. c: Histological ovarian pattern diagram of the wild ground squirrels during the breeding season. d: Histological ovarian pattern diagram of the wild ground squirrels during the nonbreeding season. e: Seasonal change of the number of primary follicles. f: Seasonal change of the number of secondary follicles. g: Seasonal change of the number of antral, post-antral follicles. h: Seasonal change of the number of corpus luteum. PF, Primary Follicle; SF, Secondary Follicle; AF, Antral Follicle; DF, Dominant Follicle; CL, Corpus Luteum. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001. Bars = 200μm.

Mentions: The ovarian histology of wild ground squirrels showed significant seasonal changes in appearance of the follicular composition between the breeding and nonbreeding seasons (Figure 1). Follicles were observed at every different stage of the follicular development in the ovaries of the breeding season, including primary follicle, secondary follicle, antral follicle, dominant follicle and corpus luteum (Figure 1a). Only primary follicles and secondary follicles were found in the ovaries of the nonbreeding season (Figure 1b). Histological pattern diagrams of ovaries were shown in Figure 1c (the breeding season) and Figure 1d (the nonbreeding season). Except for the primary follicle, the number of secondary follicles, antral follicle, post-antral follicle and corpus luteum displayed a significant decrease in the nonbreeding season when compared with the follicle number in the ovaries of the breeding season (Figure 1)e, f, g and h.


Immunoreactivities of androgen receptor, estrogen receptors, p450arom, p450c17 proteins in wild ground squirrels ovaries during the nonbreeding and breeding seasons.

Li X, Zhang H, Sheng X, Li B, Zhou J, Xu M, Weng Q, Watanabe G, Taya K - J Ovarian Res (2012)

Seasonal changes in the ovary of wild female ground squirrels.a: The histological observation and diagram of the ovary in breeding season. b: The histological observation and diagram of the ovary in nonbreeding season. c: Histological ovarian pattern diagram of the wild ground squirrels during the breeding season. d: Histological ovarian pattern diagram of the wild ground squirrels during the nonbreeding season. e: Seasonal change of the number of primary follicles. f: Seasonal change of the number of secondary follicles. g: Seasonal change of the number of antral, post-antral follicles. h: Seasonal change of the number of corpus luteum. PF, Primary Follicle; SF, Secondary Follicle; AF, Antral Follicle; DF, Dominant Follicle; CL, Corpus Luteum. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001. Bars = 200μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3473255&req=5

Figure 1: Seasonal changes in the ovary of wild female ground squirrels.a: The histological observation and diagram of the ovary in breeding season. b: The histological observation and diagram of the ovary in nonbreeding season. c: Histological ovarian pattern diagram of the wild ground squirrels during the breeding season. d: Histological ovarian pattern diagram of the wild ground squirrels during the nonbreeding season. e: Seasonal change of the number of primary follicles. f: Seasonal change of the number of secondary follicles. g: Seasonal change of the number of antral, post-antral follicles. h: Seasonal change of the number of corpus luteum. PF, Primary Follicle; SF, Secondary Follicle; AF, Antral Follicle; DF, Dominant Follicle; CL, Corpus Luteum. Data are shown as the mean ± SEM. *: p < 0.05, **: p < 0.01, ***: p < 0.001. Bars = 200μm.
Mentions: The ovarian histology of wild ground squirrels showed significant seasonal changes in appearance of the follicular composition between the breeding and nonbreeding seasons (Figure 1). Follicles were observed at every different stage of the follicular development in the ovaries of the breeding season, including primary follicle, secondary follicle, antral follicle, dominant follicle and corpus luteum (Figure 1a). Only primary follicles and secondary follicles were found in the ovaries of the nonbreeding season (Figure 1b). Histological pattern diagrams of ovaries were shown in Figure 1c (the breeding season) and Figure 1d (the nonbreeding season). Except for the primary follicle, the number of secondary follicles, antral follicle, post-antral follicle and corpus luteum displayed a significant decrease in the nonbreeding season when compared with the follicle number in the ovaries of the breeding season (Figure 1)e, f, g and h.

Bottom Line: However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season.The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons.In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China. qiangweng@bjfu.edu.cn.

ABSTRACT
The aim of this study was to elucidate the regulatory role of androgen in the follicular development of wild female ground squirrels. Immunohistochemical staining of FSHR, LHR, P450c17, P450arom, androgen receptor (AR), estrogen receptors (ERa and ERb) were executed in ovaries of female ground squirrels from both breeding and nonbreeding seasons. In addition, total ovarian proteins were extracted from the ovaries of squirrels from breeding and nonbreeding seasons, and Western blot analysis were performed in order to probe for FSHR, LHR, P450c17, P450arom, AR, ERa and ERb. The results of immunohistochemical staining and Western blotting of P450c17 showed that there was no significant difference between the breeding and nonbreeding seasons. It was found that granulosa cells expressed P450arom during the breeding season. In contrast, there was no positive staining of P450arom in the nonbreeding season. There was no significant difference in immunoreactivity of AR between the breeding and nonbreeding seasons. However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season. The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons. In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season. These findings suggested that androgen might be predominantly converted into estrogen in order to regulate the follicular development via binding of estrogen receptors during the breeding season, whereas androgen might predominantly directly bind androgen receptor to regulate the follicular development during the nonbreeding season in the ovaries of wild female ground squirrels.

No MeSH data available.


Related in: MedlinePlus