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A pilot investigation of visceral fat adiposity and gene expression profile in peripheral blood cells.

Yamaoka M, Maeda N, Nakamura S, Kashine S, Nakagawa Y, Hiuge-Shimizu A, Okita K, Imagawa A, Matsuzawa Y, Matsubara K, Funahashi T, Shimomura I - PLoS ONE (2012)

Bottom Line: The expression of several genes related to circadian rhythm, inflammation, and oxidative stress correlated significantly with visceral fat accumulation.Stepwise multiple regression analysis identified eVFA as a significant determinant of PER1 expression.In conclusion, visceral fat adiposity correlated with the expression of genes related to circadian rhythm and inflammation in peripheral blood cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.

ABSTRACT
Evidence suggests that visceral fat accumulation plays a central role in the development of metabolic syndrome. Excess visceral fat causes local chronic low-grade inflammation and dysregulation of adipocytokines, which contribute in the pathogenesis of the metabolic syndrome. These changes may affect the gene expression in peripheral blood cells. This study for the first time examined the association between visceral fat adiposity and gene expression profile in peripheral blood cells. The gene expression profile was analyzed in peripheral blood cells from 28 obese subjects by microarray analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using peripheral blood cells from 57 obese subjects. Obesity was defined as body mass index (BMI) greater than 25 kg/m(2) according to the Japanese criteria, and the estimated visceral fat area (eVFA) was measured by abdominal bioelectrical impedance. Analysis of gene expression profile was carried out with Agilent whole human genome 4 × 44 K oligo-DNA microarray. The expression of several genes related to circadian rhythm, inflammation, and oxidative stress correlated significantly with visceral fat accumulation. Period homolog 1 (PER1) mRNA level in blood cells correlated negatively with visceral fat adiposity. Stepwise multiple regression analysis identified eVFA as a significant determinant of PER1 expression. In conclusion, visceral fat adiposity correlated with the expression of genes related to circadian rhythm and inflammation in peripheral blood cells.

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Gene expression profile in peripheral blood cells.(A) Prevalence of gene probes correlated with estimated visceral fat area (eVFA). Gene ontology analysis was performed based on the microarray data. (B) Correlation between PER1 mRNA level and eVFA. Total RNAs from peripheral blood cells of 57 subjects were subjected to RT-PCR.
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pone-0047377-g001: Gene expression profile in peripheral blood cells.(A) Prevalence of gene probes correlated with estimated visceral fat area (eVFA). Gene ontology analysis was performed based on the microarray data. (B) Correlation between PER1 mRNA level and eVFA. Total RNAs from peripheral blood cells of 57 subjects were subjected to RT-PCR.

Mentions: Next, we conducted gene ontology (GO) analysis and searched for genes involved in circadian rhythm (GO:0007623), inflammation (GO:0006954), oxidative stress (GO:0006979), immune response (GO:0006955), lipid metabolism (GO:0006629), and glucose metabolism (GO:0006006). Figure 1A shows the prevalence of genes that showed significant correlation with eVFA. The number of circadian rhythm genes was small, but 5 genes (18.5%) showed significant correlation with eVFA. The frequencies of inflammation-, oxidative stress-, and immune response-related genes that correlated significantly with eVFA were 5.9%, 7.8%, and 8.8%, respectively. Furthermore, the frequencies of lipid metabolism- and glucose metabolism-related genes that correlated significantly with eVFA were 3.0% and 6.1%, respectively. Increasing evidence demonstrates a close relationship between the disturbance of circadian clock oscillator and the development of metabolic syndrome [12]–[14]. Table 3 shows gene probes related to circadian rhythm (GO:0007623). PER1, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ERBB3), clock homolog (CLOCK), prokineticin 2 (PROK2), and cryptochrome 2 (CRY2) correlated significantly with eVFA.


A pilot investigation of visceral fat adiposity and gene expression profile in peripheral blood cells.

Yamaoka M, Maeda N, Nakamura S, Kashine S, Nakagawa Y, Hiuge-Shimizu A, Okita K, Imagawa A, Matsuzawa Y, Matsubara K, Funahashi T, Shimomura I - PLoS ONE (2012)

Gene expression profile in peripheral blood cells.(A) Prevalence of gene probes correlated with estimated visceral fat area (eVFA). Gene ontology analysis was performed based on the microarray data. (B) Correlation between PER1 mRNA level and eVFA. Total RNAs from peripheral blood cells of 57 subjects were subjected to RT-PCR.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472996&req=5

pone-0047377-g001: Gene expression profile in peripheral blood cells.(A) Prevalence of gene probes correlated with estimated visceral fat area (eVFA). Gene ontology analysis was performed based on the microarray data. (B) Correlation between PER1 mRNA level and eVFA. Total RNAs from peripheral blood cells of 57 subjects were subjected to RT-PCR.
Mentions: Next, we conducted gene ontology (GO) analysis and searched for genes involved in circadian rhythm (GO:0007623), inflammation (GO:0006954), oxidative stress (GO:0006979), immune response (GO:0006955), lipid metabolism (GO:0006629), and glucose metabolism (GO:0006006). Figure 1A shows the prevalence of genes that showed significant correlation with eVFA. The number of circadian rhythm genes was small, but 5 genes (18.5%) showed significant correlation with eVFA. The frequencies of inflammation-, oxidative stress-, and immune response-related genes that correlated significantly with eVFA were 5.9%, 7.8%, and 8.8%, respectively. Furthermore, the frequencies of lipid metabolism- and glucose metabolism-related genes that correlated significantly with eVFA were 3.0% and 6.1%, respectively. Increasing evidence demonstrates a close relationship between the disturbance of circadian clock oscillator and the development of metabolic syndrome [12]–[14]. Table 3 shows gene probes related to circadian rhythm (GO:0007623). PER1, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ERBB3), clock homolog (CLOCK), prokineticin 2 (PROK2), and cryptochrome 2 (CRY2) correlated significantly with eVFA.

Bottom Line: The expression of several genes related to circadian rhythm, inflammation, and oxidative stress correlated significantly with visceral fat accumulation.Stepwise multiple regression analysis identified eVFA as a significant determinant of PER1 expression.In conclusion, visceral fat adiposity correlated with the expression of genes related to circadian rhythm and inflammation in peripheral blood cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.

ABSTRACT
Evidence suggests that visceral fat accumulation plays a central role in the development of metabolic syndrome. Excess visceral fat causes local chronic low-grade inflammation and dysregulation of adipocytokines, which contribute in the pathogenesis of the metabolic syndrome. These changes may affect the gene expression in peripheral blood cells. This study for the first time examined the association between visceral fat adiposity and gene expression profile in peripheral blood cells. The gene expression profile was analyzed in peripheral blood cells from 28 obese subjects by microarray analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using peripheral blood cells from 57 obese subjects. Obesity was defined as body mass index (BMI) greater than 25 kg/m(2) according to the Japanese criteria, and the estimated visceral fat area (eVFA) was measured by abdominal bioelectrical impedance. Analysis of gene expression profile was carried out with Agilent whole human genome 4 × 44 K oligo-DNA microarray. The expression of several genes related to circadian rhythm, inflammation, and oxidative stress correlated significantly with visceral fat accumulation. Period homolog 1 (PER1) mRNA level in blood cells correlated negatively with visceral fat adiposity. Stepwise multiple regression analysis identified eVFA as a significant determinant of PER1 expression. In conclusion, visceral fat adiposity correlated with the expression of genes related to circadian rhythm and inflammation in peripheral blood cells.

Show MeSH
Related in: MedlinePlus