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miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus

Apoptosis of CD90+ HepG2 cells after miR-548c-5p transfection. PI staining (A1, B1) showed apoptosis of CD90+ HepG2 cells increased in the transfection group at 48 h after miR-548c-5p transfection as compared to the control group. (A1) PI positive cells in the transfection group; (A2) cells under normal light in the transfection group; (B1) PI positive cells in the control group; (B2) cells under normal light in the control group. Annexin V-FITC staining (C1, D1) showed the apoptotic CD90+ HepG2 cells increased in the transfection group. (C1) Annexin V-FITC positive cells in the transfection group; (C2) cells under normal light in the transfection group; (D1) Annexin V-FITC positive cells in the control group; (D2) cells under normal light in the control group.
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f5-rado-46-03-233: Apoptosis of CD90+ HepG2 cells after miR-548c-5p transfection. PI staining (A1, B1) showed apoptosis of CD90+ HepG2 cells increased in the transfection group at 48 h after miR-548c-5p transfection as compared to the control group. (A1) PI positive cells in the transfection group; (A2) cells under normal light in the transfection group; (B1) PI positive cells in the control group; (B2) cells under normal light in the control group. Annexin V-FITC staining (C1, D1) showed the apoptotic CD90+ HepG2 cells increased in the transfection group. (C1) Annexin V-FITC positive cells in the transfection group; (C2) cells under normal light in the transfection group; (D1) Annexin V-FITC positive cells in the control group; (D2) cells under normal light in the control group.


miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

Apoptosis of CD90+ HepG2 cells after miR-548c-5p transfection. PI staining (A1, B1) showed apoptosis of CD90+ HepG2 cells increased in the transfection group at 48 h after miR-548c-5p transfection as compared to the control group. (A1) PI positive cells in the transfection group; (A2) cells under normal light in the transfection group; (B1) PI positive cells in the control group; (B2) cells under normal light in the control group. Annexin V-FITC staining (C1, D1) showed the apoptotic CD90+ HepG2 cells increased in the transfection group. (C1) Annexin V-FITC positive cells in the transfection group; (C2) cells under normal light in the transfection group; (D1) Annexin V-FITC positive cells in the control group; (D2) cells under normal light in the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472946&req=5

f5-rado-46-03-233: Apoptosis of CD90+ HepG2 cells after miR-548c-5p transfection. PI staining (A1, B1) showed apoptosis of CD90+ HepG2 cells increased in the transfection group at 48 h after miR-548c-5p transfection as compared to the control group. (A1) PI positive cells in the transfection group; (A2) cells under normal light in the transfection group; (B1) PI positive cells in the control group; (B2) cells under normal light in the control group. Annexin V-FITC staining (C1, D1) showed the apoptotic CD90+ HepG2 cells increased in the transfection group. (C1) Annexin V-FITC positive cells in the transfection group; (C2) cells under normal light in the transfection group; (D1) Annexin V-FITC positive cells in the control group; (D2) cells under normal light in the control group.
Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus