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miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus

Wound healing assay (A, B) and transwell invasion assay (C, D). (A) Wound healing assay (1): miR-548c-5p transfection group (24 h); (2): control group (24 h); (3): miR-548c-5p transfection group (48 h); (4): control group (48 h); (B) Distance of wound was correlated to cell migration ability. Results showed that cell migration was inhibited in transfection group at 24 h and 48 h after miR-548c-5p transfection (P<0.05); (C) Cells penetrating the membrane in transwell invasion assay. The number of CD90+ HepG2 cells penetrating the membrane decreased at 48 h after miR-548c-5p transfection, upper: transfection group; lower: control group; (D) Changes in the number of cells penetrating the membrane in transwell invasion assay. The number of cells penetrating the membrane was lower in the transfection group than that in the control group. *P<0. 05 vs control group.
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f4-rado-46-03-233: Wound healing assay (A, B) and transwell invasion assay (C, D). (A) Wound healing assay (1): miR-548c-5p transfection group (24 h); (2): control group (24 h); (3): miR-548c-5p transfection group (48 h); (4): control group (48 h); (B) Distance of wound was correlated to cell migration ability. Results showed that cell migration was inhibited in transfection group at 24 h and 48 h after miR-548c-5p transfection (P<0.05); (C) Cells penetrating the membrane in transwell invasion assay. The number of CD90+ HepG2 cells penetrating the membrane decreased at 48 h after miR-548c-5p transfection, upper: transfection group; lower: control group; (D) Changes in the number of cells penetrating the membrane in transwell invasion assay. The number of cells penetrating the membrane was lower in the transfection group than that in the control group. *P<0. 05 vs control group.

Mentions: The wound healing assay showed that the migration ability of CD90+ HepG2 cells was significantly compromised at 48 h after miR-548c-5p transfection when compared with untransfected CD90+ HepG2 cells (Figures 4A, B).


miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

Wound healing assay (A, B) and transwell invasion assay (C, D). (A) Wound healing assay (1): miR-548c-5p transfection group (24 h); (2): control group (24 h); (3): miR-548c-5p transfection group (48 h); (4): control group (48 h); (B) Distance of wound was correlated to cell migration ability. Results showed that cell migration was inhibited in transfection group at 24 h and 48 h after miR-548c-5p transfection (P<0.05); (C) Cells penetrating the membrane in transwell invasion assay. The number of CD90+ HepG2 cells penetrating the membrane decreased at 48 h after miR-548c-5p transfection, upper: transfection group; lower: control group; (D) Changes in the number of cells penetrating the membrane in transwell invasion assay. The number of cells penetrating the membrane was lower in the transfection group than that in the control group. *P<0. 05 vs control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472946&req=5

f4-rado-46-03-233: Wound healing assay (A, B) and transwell invasion assay (C, D). (A) Wound healing assay (1): miR-548c-5p transfection group (24 h); (2): control group (24 h); (3): miR-548c-5p transfection group (48 h); (4): control group (48 h); (B) Distance of wound was correlated to cell migration ability. Results showed that cell migration was inhibited in transfection group at 24 h and 48 h after miR-548c-5p transfection (P<0.05); (C) Cells penetrating the membrane in transwell invasion assay. The number of CD90+ HepG2 cells penetrating the membrane decreased at 48 h after miR-548c-5p transfection, upper: transfection group; lower: control group; (D) Changes in the number of cells penetrating the membrane in transwell invasion assay. The number of cells penetrating the membrane was lower in the transfection group than that in the control group. *P<0. 05 vs control group.
Mentions: The wound healing assay showed that the migration ability of CD90+ HepG2 cells was significantly compromised at 48 h after miR-548c-5p transfection when compared with untransfected CD90+ HepG2 cells (Figures 4A, B).

Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus