Limits...
miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus

miR-548c-5p inhibits CD90+ cell proliferation. IR of CD90+ HepG2 cells increased markedly following transfection and the IR increased over time. (A) OD at 24 h after miR-548c-5p transfection. OD decreased at 24 h after miR-548c-5p transfection in MTT assay; (B) OD at 48 h after miR-548c-5p transfection. OD decreased at 48 h after miR-548c-5p transfection in MTT assay; (C) OD at 72 h after miR-548c-5p transfection. OD decreased at 72 h after miR-548c-5p transfection in MTT assay; (D) IR after transfection with miR-548c-5p mimics (MTT). IR of CD90+ HepG2 cells increased markedly following transfection with miR-548c-5p mimics and IR increased over time.* 72h verses 24h, P<0.05; # 72h verses 48h, P<0.05; 48h verses 24h, P<0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3472946&req=5

f3-rado-46-03-233: miR-548c-5p inhibits CD90+ cell proliferation. IR of CD90+ HepG2 cells increased markedly following transfection and the IR increased over time. (A) OD at 24 h after miR-548c-5p transfection. OD decreased at 24 h after miR-548c-5p transfection in MTT assay; (B) OD at 48 h after miR-548c-5p transfection. OD decreased at 48 h after miR-548c-5p transfection in MTT assay; (C) OD at 72 h after miR-548c-5p transfection. OD decreased at 72 h after miR-548c-5p transfection in MTT assay; (D) IR after transfection with miR-548c-5p mimics (MTT). IR of CD90+ HepG2 cells increased markedly following transfection with miR-548c-5p mimics and IR increased over time.* 72h verses 24h, P<0.05; # 72h verses 48h, P<0.05; 48h verses 24h, P<0.05

Mentions: MTT assay was performed to examine the IR of CD90+ HepG2 cell proliferation after miR-548c-5p transfection. In miR-548c-5p transfected cells, the OD was decreased at 24 h, 48 h, and 72 h after the transfection (Figures 3A, B, C). IR of CD90+ HepG2 cells following the transfection increased markedly as compared to untransfected CD90+ HepG2 cells and the IR increased over time (Figures 3D).


miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

miR-548c-5p inhibits CD90+ cell proliferation. IR of CD90+ HepG2 cells increased markedly following transfection and the IR increased over time. (A) OD at 24 h after miR-548c-5p transfection. OD decreased at 24 h after miR-548c-5p transfection in MTT assay; (B) OD at 48 h after miR-548c-5p transfection. OD decreased at 48 h after miR-548c-5p transfection in MTT assay; (C) OD at 72 h after miR-548c-5p transfection. OD decreased at 72 h after miR-548c-5p transfection in MTT assay; (D) IR after transfection with miR-548c-5p mimics (MTT). IR of CD90+ HepG2 cells increased markedly following transfection with miR-548c-5p mimics and IR increased over time.* 72h verses 24h, P<0.05; # 72h verses 48h, P<0.05; 48h verses 24h, P<0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472946&req=5

f3-rado-46-03-233: miR-548c-5p inhibits CD90+ cell proliferation. IR of CD90+ HepG2 cells increased markedly following transfection and the IR increased over time. (A) OD at 24 h after miR-548c-5p transfection. OD decreased at 24 h after miR-548c-5p transfection in MTT assay; (B) OD at 48 h after miR-548c-5p transfection. OD decreased at 48 h after miR-548c-5p transfection in MTT assay; (C) OD at 72 h after miR-548c-5p transfection. OD decreased at 72 h after miR-548c-5p transfection in MTT assay; (D) IR after transfection with miR-548c-5p mimics (MTT). IR of CD90+ HepG2 cells increased markedly following transfection with miR-548c-5p mimics and IR increased over time.* 72h verses 24h, P<0.05; # 72h verses 48h, P<0.05; 48h verses 24h, P<0.05
Mentions: MTT assay was performed to examine the IR of CD90+ HepG2 cell proliferation after miR-548c-5p transfection. In miR-548c-5p transfected cells, the OD was decreased at 24 h, 48 h, and 72 h after the transfection (Figures 3A, B, C). IR of CD90+ HepG2 cells following the transfection increased markedly as compared to untransfected CD90+ HepG2 cells and the IR increased over time (Figures 3D).

Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus