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miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus

Protein expression of caspase-3, bcl-2 and bcl-xl after miR-548c-5p transfection. (A) Western blot assay demonstrated decreased OD of caspase-3, Bcl-2, and Bcl-XL in transfection group when compared with control group. From left to right: CD90− HepG2 group; control group; transfection group; (B) Quantification of caspase-3, bcl-2, and bcl-xl after miR-548c-5p transfection normalized to β-actin. Protein expression of caspase-3, Bcl-2, and Bcl-XL decreased significantly in transfected cells (*P<0.05).
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f2-rado-46-03-233: Protein expression of caspase-3, bcl-2 and bcl-xl after miR-548c-5p transfection. (A) Western blot assay demonstrated decreased OD of caspase-3, Bcl-2, and Bcl-XL in transfection group when compared with control group. From left to right: CD90− HepG2 group; control group; transfection group; (B) Quantification of caspase-3, bcl-2, and bcl-xl after miR-548c-5p transfection normalized to β-actin. Protein expression of caspase-3, Bcl-2, and Bcl-XL decreased significantly in transfected cells (*P<0.05).

Mentions: The western blot assay showed that protein expression of caspase-3, Bcl-2, and Bcl-XL decreased significantly in CD90+ cells transfected with miR-548c-5p when compared with untransfected CD90+ cells (Figures 2A, B).


miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.

Fang L, Zhang HB, Li H, Fu Y, Yang GS - Radiol Oncol (2012)

Protein expression of caspase-3, bcl-2 and bcl-xl after miR-548c-5p transfection. (A) Western blot assay demonstrated decreased OD of caspase-3, Bcl-2, and Bcl-XL in transfection group when compared with control group. From left to right: CD90− HepG2 group; control group; transfection group; (B) Quantification of caspase-3, bcl-2, and bcl-xl after miR-548c-5p transfection normalized to β-actin. Protein expression of caspase-3, Bcl-2, and Bcl-XL decreased significantly in transfected cells (*P<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472946&req=5

f2-rado-46-03-233: Protein expression of caspase-3, bcl-2 and bcl-xl after miR-548c-5p transfection. (A) Western blot assay demonstrated decreased OD of caspase-3, Bcl-2, and Bcl-XL in transfection group when compared with control group. From left to right: CD90− HepG2 group; control group; transfection group; (B) Quantification of caspase-3, bcl-2, and bcl-xl after miR-548c-5p transfection normalized to β-actin. Protein expression of caspase-3, Bcl-2, and Bcl-XL decreased significantly in transfected cells (*P<0.05).
Mentions: The western blot assay showed that protein expression of caspase-3, Bcl-2, and Bcl-XL decreased significantly in CD90+ cells transfected with miR-548c-5p when compared with untransfected CD90+ cells (Figures 2A, B).

Bottom Line: The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study.Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells.Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Centre, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai. China.

ABSTRACT

Background: Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.

Results: Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.

Conclusions: Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.

No MeSH data available.


Related in: MedlinePlus