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Lucifer Yellow uptake by CHO cells exposed to magnetic and electric pulses.

Towhidi L, Firoozabadi SM, Mozdarani H, Miklavcic D - Radiol Oncol (2012)

Bottom Line: The Chinese hamster ovary (CHO) cells were exposed to magnetic pulses of 2.2 T peak strength and 250 μs duration delivered by Magstim stimulator and double 70 mm coil.Three different frequencies of 0.25, 1 and 10 Hz pulses with 112, 56 and 28 number of pulses were applied (altogether nine experimental groups) and Lucifer Yellow uptake was measured in each group.Our results show that time-varying magnetic field exposure increases transmembrane molecular transport and this uptake is greater for lower frequencies and larger number of pulses.

View Article: PubMed Central - PubMed

Affiliation: Tarbiat Modares University, Department of Medical Physics, Tehran, Iran ; University of Ljubljana, Faculty of Electrical Engineering, Ljubljana, Slovenia.

ABSTRACT

Background: The cell membrane acts as a barrier that hinders free entrance of most hydrophilic molecules into the cell. Due to numerous applications in medicine, biology and biotechnology, the introduction of impermeant molecules into biological cells has drawn considerable attention in the past years. One of the most famous methods in this field is electroporation, in which electric pulses with high intensity and short duration are applied to the cells. The aim of our study was to investigate the effect of time-varying magnetic field with different parameters on transmembrane molecular transport.

Materials and methods: 'Moreover, a comparison was made between the uptake results due to magnetic pulse exposure and electroporation mediated uptake.' at the end of Background part. The Chinese hamster ovary (CHO) cells were exposed to magnetic pulses of 2.2 T peak strength and 250 μs duration delivered by Magstim stimulator and double 70 mm coil. Three different frequencies of 0.25, 1 and 10 Hz pulses with 112, 56 and 28 number of pulses were applied (altogether nine experimental groups) and Lucifer Yellow uptake was measured in each group. Moreover, maximum uptake of Lucifer Yellow obtained by magnetic pulses was compared to the measured uptake due to electroporation with typical parameters of 8 pulses of 100 μs, repetition frequency of 1 Hz and electric field intensities of 200 to 600 V/cm.

Results and conclusions: Our results show that time-varying magnetic field exposure increases transmembrane molecular transport and this uptake is greater for lower frequencies and larger number of pulses. Besides, the comparison shows that electroporation is more effective than pulsed magnetic field, but the observed uptake enhancement due to magnetic exposure is still considerable.

No MeSH data available.


Related in: MedlinePlus

Dye uptake of attached CHO cells for three different frequencies 0.25, 1 and 10 Hz with three different numbers of pulses 112, 56 and 28 for each chosen frequency. Attributed number to the control group was chosen 100 and the fluorescence of other groups was computed as the percent of control fluorescence.
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f2-rado-46-02-119: Dye uptake of attached CHO cells for three different frequencies 0.25, 1 and 10 Hz with three different numbers of pulses 112, 56 and 28 for each chosen frequency. Attributed number to the control group was chosen 100 and the fluorescence of other groups was computed as the percent of control fluorescence.

Mentions: Figure 2 shows the uptake of Lucifer Yellow for 9 different sets of parameters of magnetic field exposure (three different frequencies of 0.25, 1 and 10 Hz pulses for 112, 56 and 28 pulses). The value of the control group fluorescence was considered as 100 and the fluorescence of experimental groups was normalized to the control group. The comparisons of the uptake between the control group and exposed groups and also between exposed groups were performed using the Mann-Whitney Rank-Sum test. The P-value for the significance level of distinct groups was set equal to 0.05.


Lucifer Yellow uptake by CHO cells exposed to magnetic and electric pulses.

Towhidi L, Firoozabadi SM, Mozdarani H, Miklavcic D - Radiol Oncol (2012)

Dye uptake of attached CHO cells for three different frequencies 0.25, 1 and 10 Hz with three different numbers of pulses 112, 56 and 28 for each chosen frequency. Attributed number to the control group was chosen 100 and the fluorescence of other groups was computed as the percent of control fluorescence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472937&req=5

f2-rado-46-02-119: Dye uptake of attached CHO cells for three different frequencies 0.25, 1 and 10 Hz with three different numbers of pulses 112, 56 and 28 for each chosen frequency. Attributed number to the control group was chosen 100 and the fluorescence of other groups was computed as the percent of control fluorescence.
Mentions: Figure 2 shows the uptake of Lucifer Yellow for 9 different sets of parameters of magnetic field exposure (three different frequencies of 0.25, 1 and 10 Hz pulses for 112, 56 and 28 pulses). The value of the control group fluorescence was considered as 100 and the fluorescence of experimental groups was normalized to the control group. The comparisons of the uptake between the control group and exposed groups and also between exposed groups were performed using the Mann-Whitney Rank-Sum test. The P-value for the significance level of distinct groups was set equal to 0.05.

Bottom Line: The Chinese hamster ovary (CHO) cells were exposed to magnetic pulses of 2.2 T peak strength and 250 μs duration delivered by Magstim stimulator and double 70 mm coil.Three different frequencies of 0.25, 1 and 10 Hz pulses with 112, 56 and 28 number of pulses were applied (altogether nine experimental groups) and Lucifer Yellow uptake was measured in each group.Our results show that time-varying magnetic field exposure increases transmembrane molecular transport and this uptake is greater for lower frequencies and larger number of pulses.

View Article: PubMed Central - PubMed

Affiliation: Tarbiat Modares University, Department of Medical Physics, Tehran, Iran ; University of Ljubljana, Faculty of Electrical Engineering, Ljubljana, Slovenia.

ABSTRACT

Background: The cell membrane acts as a barrier that hinders free entrance of most hydrophilic molecules into the cell. Due to numerous applications in medicine, biology and biotechnology, the introduction of impermeant molecules into biological cells has drawn considerable attention in the past years. One of the most famous methods in this field is electroporation, in which electric pulses with high intensity and short duration are applied to the cells. The aim of our study was to investigate the effect of time-varying magnetic field with different parameters on transmembrane molecular transport.

Materials and methods: 'Moreover, a comparison was made between the uptake results due to magnetic pulse exposure and electroporation mediated uptake.' at the end of Background part. The Chinese hamster ovary (CHO) cells were exposed to magnetic pulses of 2.2 T peak strength and 250 μs duration delivered by Magstim stimulator and double 70 mm coil. Three different frequencies of 0.25, 1 and 10 Hz pulses with 112, 56 and 28 number of pulses were applied (altogether nine experimental groups) and Lucifer Yellow uptake was measured in each group. Moreover, maximum uptake of Lucifer Yellow obtained by magnetic pulses was compared to the measured uptake due to electroporation with typical parameters of 8 pulses of 100 μs, repetition frequency of 1 Hz and electric field intensities of 200 to 600 V/cm.

Results and conclusions: Our results show that time-varying magnetic field exposure increases transmembrane molecular transport and this uptake is greater for lower frequencies and larger number of pulses. Besides, the comparison shows that electroporation is more effective than pulsed magnetic field, but the observed uptake enhancement due to magnetic exposure is still considerable.

No MeSH data available.


Related in: MedlinePlus