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Miniaturized integrated platform for electrical and optical monitoring of cell cultures.

Moldovan C, Iosub R, Codreanu C, Firtat B, Necula D, Brasoveanu C, Stan I - Sensors (Basel) (2012)

Bottom Line: Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads.The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells.The fabrication steps are presented, along with the electrical and optical characterization of the system.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research and Development in Microtechnologies, 126 Erou Iancu Nicolae, Bucharest 077190, Romania. carmen.moldovan@imt.ro

ABSTRACT
The following paper describes the design and functions of a miniaturized integrated platform for optical and electrical monitoring of cell cultures and the necessary steps in the fabrication and testing of a silicon microchip Micro ElectroMechanical Systems (MEMS)-based technology for cell data recording, monitoring and stimulation. The silicon microchip consists of a MEMS machined device containing a shank of 240 μm width, 3 mm long and 50 μm thick and an enlarged area of 5 mm × 5 mm hosting the pads for electrical connections. Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads. The sensors aim to monitor the pH, the temperature and the impedance of the cell culture. The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells. The fabrication steps are presented, along with the electrical and optical characterization of the system. The target of the research is to develop a new and reconfigurable platform according to the particular applications needs, as a tool for the biologist, chemists and medical doctors working is the field of cell culture monitoring in terms of growth, maintenance conditions, reaction to electrical or chemical stimulation (drugs, toxicants, etc.). HaCaT (Immortalised Human Keratinocyte) cell culture has been used for demonstration purposes in order to provide information on the platform electrical and optical functions.

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Mini-incubator for monitoring of cell cultures.
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f8-sensors-12-11372: Mini-incubator for monitoring of cell cultures.

Mentions: The part that we call mini-incubator (presented in Figure 8) contains a special glass vessel of 4 cm in diameter and a special polymeric cap. The glass vessel is thin and as much as possible transparent, allowing a good resolution of pictures taken by a video camera placed underneath (Figure 8). The vessel is provided with two inlets and one outlet connected to the fluidic system (tubes and pumps) for circulation of needed liquids inside the vessel. The fluid circulation was tested in connection with the temperature control inside the incubator. The special polymeric cap is built to be fixed on the glass vessel and has an opening on the top side for microprobe insertion inside. The cap is manufactured to have a very flexible and extendable part allowing the positioning and alignment of the microprobe on Z direction. The vessel and the cap can be sterilized both in temperature (>100 °C) and in chemical solutions without damages or deformations.


Miniaturized integrated platform for electrical and optical monitoring of cell cultures.

Moldovan C, Iosub R, Codreanu C, Firtat B, Necula D, Brasoveanu C, Stan I - Sensors (Basel) (2012)

Mini-incubator for monitoring of cell cultures.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472889&req=5

f8-sensors-12-11372: Mini-incubator for monitoring of cell cultures.
Mentions: The part that we call mini-incubator (presented in Figure 8) contains a special glass vessel of 4 cm in diameter and a special polymeric cap. The glass vessel is thin and as much as possible transparent, allowing a good resolution of pictures taken by a video camera placed underneath (Figure 8). The vessel is provided with two inlets and one outlet connected to the fluidic system (tubes and pumps) for circulation of needed liquids inside the vessel. The fluid circulation was tested in connection with the temperature control inside the incubator. The special polymeric cap is built to be fixed on the glass vessel and has an opening on the top side for microprobe insertion inside. The cap is manufactured to have a very flexible and extendable part allowing the positioning and alignment of the microprobe on Z direction. The vessel and the cap can be sterilized both in temperature (>100 °C) and in chemical solutions without damages or deformations.

Bottom Line: Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads.The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells.The fabrication steps are presented, along with the electrical and optical characterization of the system.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research and Development in Microtechnologies, 126 Erou Iancu Nicolae, Bucharest 077190, Romania. carmen.moldovan@imt.ro

ABSTRACT
The following paper describes the design and functions of a miniaturized integrated platform for optical and electrical monitoring of cell cultures and the necessary steps in the fabrication and testing of a silicon microchip Micro ElectroMechanical Systems (MEMS)-based technology for cell data recording, monitoring and stimulation. The silicon microchip consists of a MEMS machined device containing a shank of 240 μm width, 3 mm long and 50 μm thick and an enlarged area of 5 mm × 5 mm hosting the pads for electrical connections. Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads. The sensors aim to monitor the pH, the temperature and the impedance of the cell culture. The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells. The fabrication steps are presented, along with the electrical and optical characterization of the system. The target of the research is to develop a new and reconfigurable platform according to the particular applications needs, as a tool for the biologist, chemists and medical doctors working is the field of cell culture monitoring in terms of growth, maintenance conditions, reaction to electrical or chemical stimulation (drugs, toxicants, etc.). HaCaT (Immortalised Human Keratinocyte) cell culture has been used for demonstration purposes in order to provide information on the platform electrical and optical functions.

Show MeSH
Related in: MedlinePlus