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Miniaturized integrated platform for electrical and optical monitoring of cell cultures.

Moldovan C, Iosub R, Codreanu C, Firtat B, Necula D, Brasoveanu C, Stan I - Sensors (Basel) (2012)

Bottom Line: Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads.The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells.The fabrication steps are presented, along with the electrical and optical characterization of the system.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research and Development in Microtechnologies, 126 Erou Iancu Nicolae, Bucharest 077190, Romania. carmen.moldovan@imt.ro

ABSTRACT
The following paper describes the design and functions of a miniaturized integrated platform for optical and electrical monitoring of cell cultures and the necessary steps in the fabrication and testing of a silicon microchip Micro ElectroMechanical Systems (MEMS)-based technology for cell data recording, monitoring and stimulation. The silicon microchip consists of a MEMS machined device containing a shank of 240 μm width, 3 mm long and 50 μm thick and an enlarged area of 5 mm × 5 mm hosting the pads for electrical connections. Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads. The sensors aim to monitor the pH, the temperature and the impedance of the cell culture. The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells. The fabrication steps are presented, along with the electrical and optical characterization of the system. The target of the research is to develop a new and reconfigurable platform according to the particular applications needs, as a tool for the biologist, chemists and medical doctors working is the field of cell culture monitoring in terms of growth, maintenance conditions, reaction to electrical or chemical stimulation (drugs, toxicants, etc.). HaCaT (Immortalised Human Keratinocyte) cell culture has been used for demonstration purposes in order to provide information on the platform electrical and optical functions.

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Related in: MedlinePlus

Assembled microprobe on a PCB (A) and PCB layout (B) center figure.
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f6-sensors-12-11372: Assembled microprobe on a PCB (A) and PCB layout (B) center figure.

Mentions: The electrodes and sensors for cells monitoring placed on top of the microprobe shank are designed to be inserted into the media (in vitro or in-vivo) [18]. The silicon microprobe is assembled on a printed board as shown in Figure 6, designed to accommodate the microprobe chip assembly and connection of sensors to electronic modules and PC and allowing the easy manipulation with minimum cells damage. The microprobe uses the wire bonding technique for connecting the electrodes to the outside world (electronics). For easy interfacing of the PCB board with the command electronics and measurement instruments standard microconnectors and ribbon cables have been used (Figure 6). In addition to robustness and versatility, this solution ensures the accuracy and precision of measurements by minimization of the leakage and noise components induced by connections.


Miniaturized integrated platform for electrical and optical monitoring of cell cultures.

Moldovan C, Iosub R, Codreanu C, Firtat B, Necula D, Brasoveanu C, Stan I - Sensors (Basel) (2012)

Assembled microprobe on a PCB (A) and PCB layout (B) center figure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472889&req=5

f6-sensors-12-11372: Assembled microprobe on a PCB (A) and PCB layout (B) center figure.
Mentions: The electrodes and sensors for cells monitoring placed on top of the microprobe shank are designed to be inserted into the media (in vitro or in-vivo) [18]. The silicon microprobe is assembled on a printed board as shown in Figure 6, designed to accommodate the microprobe chip assembly and connection of sensors to electronic modules and PC and allowing the easy manipulation with minimum cells damage. The microprobe uses the wire bonding technique for connecting the electrodes to the outside world (electronics). For easy interfacing of the PCB board with the command electronics and measurement instruments standard microconnectors and ribbon cables have been used (Figure 6). In addition to robustness and versatility, this solution ensures the accuracy and precision of measurements by minimization of the leakage and noise components induced by connections.

Bottom Line: Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads.The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells.The fabrication steps are presented, along with the electrical and optical characterization of the system.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research and Development in Microtechnologies, 126 Erou Iancu Nicolae, Bucharest 077190, Romania. carmen.moldovan@imt.ro

ABSTRACT
The following paper describes the design and functions of a miniaturized integrated platform for optical and electrical monitoring of cell cultures and the necessary steps in the fabrication and testing of a silicon microchip Micro ElectroMechanical Systems (MEMS)-based technology for cell data recording, monitoring and stimulation. The silicon microchip consists of a MEMS machined device containing a shank of 240 μm width, 3 mm long and 50 μm thick and an enlarged area of 5 mm × 5 mm hosting the pads for electrical connections. Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads. The sensors aim to monitor the pH, the temperature and the impedance of the cell culture. The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells. The fabrication steps are presented, along with the electrical and optical characterization of the system. The target of the research is to develop a new and reconfigurable platform according to the particular applications needs, as a tool for the biologist, chemists and medical doctors working is the field of cell culture monitoring in terms of growth, maintenance conditions, reaction to electrical or chemical stimulation (drugs, toxicants, etc.). HaCaT (Immortalised Human Keratinocyte) cell culture has been used for demonstration purposes in order to provide information on the platform electrical and optical functions.

Show MeSH
Related in: MedlinePlus