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Miniaturized integrated platform for electrical and optical monitoring of cell cultures.

Moldovan C, Iosub R, Codreanu C, Firtat B, Necula D, Brasoveanu C, Stan I - Sensors (Basel) (2012)

Bottom Line: Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads.The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells.The fabrication steps are presented, along with the electrical and optical characterization of the system.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research and Development in Microtechnologies, 126 Erou Iancu Nicolae, Bucharest 077190, Romania. carmen.moldovan@imt.ro

ABSTRACT
The following paper describes the design and functions of a miniaturized integrated platform for optical and electrical monitoring of cell cultures and the necessary steps in the fabrication and testing of a silicon microchip Micro ElectroMechanical Systems (MEMS)-based technology for cell data recording, monitoring and stimulation. The silicon microchip consists of a MEMS machined device containing a shank of 240 μm width, 3 mm long and 50 μm thick and an enlarged area of 5 mm × 5 mm hosting the pads for electrical connections. Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads. The sensors aim to monitor the pH, the temperature and the impedance of the cell culture. The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells. The fabrication steps are presented, along with the electrical and optical characterization of the system. The target of the research is to develop a new and reconfigurable platform according to the particular applications needs, as a tool for the biologist, chemists and medical doctors working is the field of cell culture monitoring in terms of growth, maintenance conditions, reaction to electrical or chemical stimulation (drugs, toxicants, etc.). HaCaT (Immortalised Human Keratinocyte) cell culture has been used for demonstration purposes in order to provide information on the platform electrical and optical functions.

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Related in: MedlinePlus

pH sensor diagram, voltage versus time in pH 7 buffer solution.
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f14-sensors-12-11372: pH sensor diagram, voltage versus time in pH 7 buffer solution.

Mentions: The conductivity increases in strong acid medium like pH 4 compared with pH 7 medium and is maximum at pH 1 and remains constant at this maximum value. Polyaniline variation depends on the pH environment and that way the sensitivity as a pH sensor is demonstrated. The conductivity of the electrode changes over three hours both in pH 4 and pH 7 as can be seen in Figure 13. The pH sensor become more stable after ∼three hours and it provided reproducible results over several days. The interpretation of this result is that the polyaniline suffers a stabilization process when it changes from one state to another state. This remark has been proven by the pH measurement for 30 hours in pH 7 buffer solution (Figure 14). After 200 minutes, the pH sensor shows a good stability and the measured voltage was constantly around 150 mV ± 5%.


Miniaturized integrated platform for electrical and optical monitoring of cell cultures.

Moldovan C, Iosub R, Codreanu C, Firtat B, Necula D, Brasoveanu C, Stan I - Sensors (Basel) (2012)

pH sensor diagram, voltage versus time in pH 7 buffer solution.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472889&req=5

f14-sensors-12-11372: pH sensor diagram, voltage versus time in pH 7 buffer solution.
Mentions: The conductivity increases in strong acid medium like pH 4 compared with pH 7 medium and is maximum at pH 1 and remains constant at this maximum value. Polyaniline variation depends on the pH environment and that way the sensitivity as a pH sensor is demonstrated. The conductivity of the electrode changes over three hours both in pH 4 and pH 7 as can be seen in Figure 13. The pH sensor become more stable after ∼three hours and it provided reproducible results over several days. The interpretation of this result is that the polyaniline suffers a stabilization process when it changes from one state to another state. This remark has been proven by the pH measurement for 30 hours in pH 7 buffer solution (Figure 14). After 200 minutes, the pH sensor shows a good stability and the measured voltage was constantly around 150 mV ± 5%.

Bottom Line: Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads.The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells.The fabrication steps are presented, along with the electrical and optical characterization of the system.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research and Development in Microtechnologies, 126 Erou Iancu Nicolae, Bucharest 077190, Romania. carmen.moldovan@imt.ro

ABSTRACT
The following paper describes the design and functions of a miniaturized integrated platform for optical and electrical monitoring of cell cultures and the necessary steps in the fabrication and testing of a silicon microchip Micro ElectroMechanical Systems (MEMS)-based technology for cell data recording, monitoring and stimulation. The silicon microchip consists of a MEMS machined device containing a shank of 240 μm width, 3 mm long and 50 μm thick and an enlarged area of 5 mm × 5 mm hosting the pads for electrical connections. Ten platinum electrodes and five sensors are placed on the shank and are connected with the external electronics through the pads. The sensors aim to monitor the pH, the temperature and the impedance of the cell culture. The electrodes are bidirectional and can be used both for electrical potential recording and stimulation of cells. The fabrication steps are presented, along with the electrical and optical characterization of the system. The target of the research is to develop a new and reconfigurable platform according to the particular applications needs, as a tool for the biologist, chemists and medical doctors working is the field of cell culture monitoring in terms of growth, maintenance conditions, reaction to electrical or chemical stimulation (drugs, toxicants, etc.). HaCaT (Immortalised Human Keratinocyte) cell culture has been used for demonstration purposes in order to provide information on the platform electrical and optical functions.

Show MeSH
Related in: MedlinePlus