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Biotin-functionalized semiconducting polymer in an organic field effect transistor and application as a biosensor.

Kim ZS, Lim SC, Kim SH, Yang YS, Hwang DH - Sensors (Basel) (2012)

Bottom Line: The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2.The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors.Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

View Article: PubMed Central - PubMed

Affiliation: Electronics and Telecommunications Research Institute (ETRI), 218 Gajeongno, Yuseong-Gu, Daejeon 305-700, Korea. zinsig.kim@gmail.com

ABSTRACT
This report presents biotin-functionalized semiconducting polymers that are based on fluorene and bithiophene co-polymers (F8T2). Also presented is the application of these polymers to an organic thin film transistor used as a biosensor. The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2. Their properties as an organic semiconductor were tested using an organic thin film transistor (OTFT) and were found to show typical p-type semiconductor curves. The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors. Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

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UV (365 nm) fluorescence test with BSA- or avidin-treated biotinylated F8T2. (A) biotinylated F8T2 with Avidin in dioxane. (B,C) biotinylated F8T2 with Avidin in MeOH. (D) biotinylated F8T2 with BSA in MeOH. (E) biotinylated F8T2 in p-Xylene. (F) Avidin in water. (G) BSA in water (up: UV light off; down: UV light on).
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f6-sensors-12-11238: UV (365 nm) fluorescence test with BSA- or avidin-treated biotinylated F8T2. (A) biotinylated F8T2 with Avidin in dioxane. (B,C) biotinylated F8T2 with Avidin in MeOH. (D) biotinylated F8T2 with BSA in MeOH. (E) biotinylated F8T2 in p-Xylene. (F) Avidin in water. (G) BSA in water (up: UV light off; down: UV light on).

Mentions: In this research, we also investigated the optical properties of the biotinylated F8T2 semiconductor in an organic solvent, such as p-xylene, methanol and dioxane (Figure 6). The biotinylated F8T2 polymer solutions in p-xylene itself showed intensive fluorescence with yellow-green color under 365 nm UV light, but without UV light the solution showed yellow color without fluorescence. After treatment with the BSA solution, the biotinylated F8T2 solution had hardly changed color without UV light and the fluorescence changed slightly from yellow to greenish-yellow under 365 nm UV light. In comparison with this, the fluorescent color of the solution, which was treated with the avidin solution, shifted remarkably from yellow/green to blue in dioxane and in methanol. The two different kinds of organic solvents, methanol and dioxane, had no remarkable influence on fluorescence effects. As a reference of the fluorescence change, the BSA and avidin solution alone indicated no fluorescence under 365 nm UV light.


Biotin-functionalized semiconducting polymer in an organic field effect transistor and application as a biosensor.

Kim ZS, Lim SC, Kim SH, Yang YS, Hwang DH - Sensors (Basel) (2012)

UV (365 nm) fluorescence test with BSA- or avidin-treated biotinylated F8T2. (A) biotinylated F8T2 with Avidin in dioxane. (B,C) biotinylated F8T2 with Avidin in MeOH. (D) biotinylated F8T2 with BSA in MeOH. (E) biotinylated F8T2 in p-Xylene. (F) Avidin in water. (G) BSA in water (up: UV light off; down: UV light on).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472882&req=5

f6-sensors-12-11238: UV (365 nm) fluorescence test with BSA- or avidin-treated biotinylated F8T2. (A) biotinylated F8T2 with Avidin in dioxane. (B,C) biotinylated F8T2 with Avidin in MeOH. (D) biotinylated F8T2 with BSA in MeOH. (E) biotinylated F8T2 in p-Xylene. (F) Avidin in water. (G) BSA in water (up: UV light off; down: UV light on).
Mentions: In this research, we also investigated the optical properties of the biotinylated F8T2 semiconductor in an organic solvent, such as p-xylene, methanol and dioxane (Figure 6). The biotinylated F8T2 polymer solutions in p-xylene itself showed intensive fluorescence with yellow-green color under 365 nm UV light, but without UV light the solution showed yellow color without fluorescence. After treatment with the BSA solution, the biotinylated F8T2 solution had hardly changed color without UV light and the fluorescence changed slightly from yellow to greenish-yellow under 365 nm UV light. In comparison with this, the fluorescent color of the solution, which was treated with the avidin solution, shifted remarkably from yellow/green to blue in dioxane and in methanol. The two different kinds of organic solvents, methanol and dioxane, had no remarkable influence on fluorescence effects. As a reference of the fluorescence change, the BSA and avidin solution alone indicated no fluorescence under 365 nm UV light.

Bottom Line: The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2.The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors.Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

View Article: PubMed Central - PubMed

Affiliation: Electronics and Telecommunications Research Institute (ETRI), 218 Gajeongno, Yuseong-Gu, Daejeon 305-700, Korea. zinsig.kim@gmail.com

ABSTRACT
This report presents biotin-functionalized semiconducting polymers that are based on fluorene and bithiophene co-polymers (F8T2). Also presented is the application of these polymers to an organic thin film transistor used as a biosensor. The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2. Their properties as an organic semiconductor were tested using an organic thin film transistor (OTFT) and were found to show typical p-type semiconductor curves. The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors. Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

Show MeSH
Related in: MedlinePlus