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Biotin-functionalized semiconducting polymer in an organic field effect transistor and application as a biosensor.

Kim ZS, Lim SC, Kim SH, Yang YS, Hwang DH - Sensors (Basel) (2012)

Bottom Line: The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2.The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors.Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

View Article: PubMed Central - PubMed

Affiliation: Electronics and Telecommunications Research Institute (ETRI), 218 Gajeongno, Yuseong-Gu, Daejeon 305-700, Korea. zinsig.kim@gmail.com

ABSTRACT
This report presents biotin-functionalized semiconducting polymers that are based on fluorene and bithiophene co-polymers (F8T2). Also presented is the application of these polymers to an organic thin film transistor used as a biosensor. The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2. Their properties as an organic semiconductor were tested using an organic thin film transistor (OTFT) and were found to show typical p-type semiconductor curves. The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors. Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

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Changes of the drain current of OTFT by the detection of sensing materials. (No): after treatment with DI water. (BSA): after treatment with BSA-solution. (Avidin): after treatment with avidin-solution.
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f5-sensors-12-11238: Changes of the drain current of OTFT by the detection of sensing materials. (No): after treatment with DI water. (BSA): after treatment with BSA-solution. (Avidin): after treatment with avidin-solution.

Mentions: Changes of the drain current of OTFT with biotinylated F8T2 by detection of sensing materials are shown in Figure 5. The drain current of the OTFT device after treatment with only DI water is indicated as “No” in Figure 5. The drain current of the OTFT device after treatment with BSA solution is indicated as “BSA”, and it decreased slightly. The drain current of the OTFT device after treatment with the avidin solution is indicated as “Avidin”, and it decreased significantly by a five order scale. In Figure 5, the mean values of the drain current of 30 samples of OTFT devices for each sensing material are indicated. The decrease of drain current after avidin treatment lay far beyond the error bar of the measurement.


Biotin-functionalized semiconducting polymer in an organic field effect transistor and application as a biosensor.

Kim ZS, Lim SC, Kim SH, Yang YS, Hwang DH - Sensors (Basel) (2012)

Changes of the drain current of OTFT by the detection of sensing materials. (No): after treatment with DI water. (BSA): after treatment with BSA-solution. (Avidin): after treatment with avidin-solution.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472882&req=5

f5-sensors-12-11238: Changes of the drain current of OTFT by the detection of sensing materials. (No): after treatment with DI water. (BSA): after treatment with BSA-solution. (Avidin): after treatment with avidin-solution.
Mentions: Changes of the drain current of OTFT with biotinylated F8T2 by detection of sensing materials are shown in Figure 5. The drain current of the OTFT device after treatment with only DI water is indicated as “No” in Figure 5. The drain current of the OTFT device after treatment with BSA solution is indicated as “BSA”, and it decreased slightly. The drain current of the OTFT device after treatment with the avidin solution is indicated as “Avidin”, and it decreased significantly by a five order scale. In Figure 5, the mean values of the drain current of 30 samples of OTFT devices for each sensing material are indicated. The decrease of drain current after avidin treatment lay far beyond the error bar of the measurement.

Bottom Line: The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2.The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors.Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

View Article: PubMed Central - PubMed

Affiliation: Electronics and Telecommunications Research Institute (ETRI), 218 Gajeongno, Yuseong-Gu, Daejeon 305-700, Korea. zinsig.kim@gmail.com

ABSTRACT
This report presents biotin-functionalized semiconducting polymers that are based on fluorene and bithiophene co-polymers (F8T2). Also presented is the application of these polymers to an organic thin film transistor used as a biosensor. The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2. Their properties as an organic semiconductor were tested using an organic thin film transistor (OTFT) and were found to show typical p-type semiconductor curves. The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors. Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

Show MeSH