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Biotin-functionalized semiconducting polymer in an organic field effect transistor and application as a biosensor.

Kim ZS, Lim SC, Kim SH, Yang YS, Hwang DH - Sensors (Basel) (2012)

Bottom Line: The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2.The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors.Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

View Article: PubMed Central - PubMed

Affiliation: Electronics and Telecommunications Research Institute (ETRI), 218 Gajeongno, Yuseong-Gu, Daejeon 305-700, Korea. zinsig.kim@gmail.com

ABSTRACT
This report presents biotin-functionalized semiconducting polymers that are based on fluorene and bithiophene co-polymers (F8T2). Also presented is the application of these polymers to an organic thin film transistor used as a biosensor. The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2. Their properties as an organic semiconductor were tested using an organic thin film transistor (OTFT) and were found to show typical p-type semiconductor curves. The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors. Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

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The typical plot of drain current ID versus gate voltage VG for biotinylated F8T2 TFTs. (a) Typical plot of the drain current ID versus gate voltage VG between 0 and '40 V. and (b) The On/Off-curve of the organic semiconductor field effect transistor (organic FET).
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f3-sensors-12-11238: The typical plot of drain current ID versus gate voltage VG for biotinylated F8T2 TFTs. (a) Typical plot of the drain current ID versus gate voltage VG between 0 and '40 V. and (b) The On/Off-curve of the organic semiconductor field effect transistor (organic FET).

Mentions: The fabricated OTFT devices in this research were characterized by generally accepted methods. According to the cross-sectional SEM image (not shown in this report), the thickness of the biotinylated F8T2 films was approximately 4,300 Å [31]. After annealing at 250 °C for 1 h under N2 atmosphere, the OTFT devices showed the typical I-V-curves of OTFTs. The VG is between 0 V and '40 V. Figure 3 shows the typical plots of drain current ID versus gate voltage VG at a drain voltage VD of '60 V for biotinylated F8T2 OTFTs, which were fabricated with a channel length of 10 μm, channel width of 100 μm, SiO2 gate dielectric thickness of 300 nm, and the On/Off-curve of the organic semiconductor field effect transistor (organic FET). Fabrication of the OTFT device was completed before exposing the compound to detecting materials, for example, BSA or avidin.


Biotin-functionalized semiconducting polymer in an organic field effect transistor and application as a biosensor.

Kim ZS, Lim SC, Kim SH, Yang YS, Hwang DH - Sensors (Basel) (2012)

The typical plot of drain current ID versus gate voltage VG for biotinylated F8T2 TFTs. (a) Typical plot of the drain current ID versus gate voltage VG between 0 and '40 V. and (b) The On/Off-curve of the organic semiconductor field effect transistor (organic FET).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472882&req=5

f3-sensors-12-11238: The typical plot of drain current ID versus gate voltage VG for biotinylated F8T2 TFTs. (a) Typical plot of the drain current ID versus gate voltage VG between 0 and '40 V. and (b) The On/Off-curve of the organic semiconductor field effect transistor (organic FET).
Mentions: The fabricated OTFT devices in this research were characterized by generally accepted methods. According to the cross-sectional SEM image (not shown in this report), the thickness of the biotinylated F8T2 films was approximately 4,300 Å [31]. After annealing at 250 °C for 1 h under N2 atmosphere, the OTFT devices showed the typical I-V-curves of OTFTs. The VG is between 0 V and '40 V. Figure 3 shows the typical plots of drain current ID versus gate voltage VG at a drain voltage VD of '60 V for biotinylated F8T2 OTFTs, which were fabricated with a channel length of 10 μm, channel width of 100 μm, SiO2 gate dielectric thickness of 300 nm, and the On/Off-curve of the organic semiconductor field effect transistor (organic FET). Fabrication of the OTFT device was completed before exposing the compound to detecting materials, for example, BSA or avidin.

Bottom Line: The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2.The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors.Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

View Article: PubMed Central - PubMed

Affiliation: Electronics and Telecommunications Research Institute (ETRI), 218 Gajeongno, Yuseong-Gu, Daejeon 305-700, Korea. zinsig.kim@gmail.com

ABSTRACT
This report presents biotin-functionalized semiconducting polymers that are based on fluorene and bithiophene co-polymers (F8T2). Also presented is the application of these polymers to an organic thin film transistor used as a biosensor. The side chains of fluorene were partially biotinylated after the esterification of the biotin with corresponding alcohol-groups at the side chain in F8T2. Their properties as an organic semiconductor were tested using an organic thin film transistor (OTFT) and were found to show typical p-type semiconductor curves. The functionality of this biosensor in the sensing of biologically active molecules such as avidin in comparison with bovine serum albumin (BSA) was established through a selective decrease in the conductivity of the transistor, as measured with a device that was developed by the authors. Changes to the optical properties of this polymer were also measured through the change in the color of the UV-fluorescence before and after a reaction with avidin or BSA.

Show MeSH