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Development of a plastic-based microfluidic immunosensor chip for detection of H1N1 influenza.

Lee KG, Lee TJ, Jeong SW, Choi HW, Heo NS, Park JY, Park TJ, Lee SJ - Sensors (Basel) (2012)

Bottom Line: A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique.For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip.This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.

View Article: PubMed Central - PubMed

Affiliation: Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea. kglee@nnfc.re.kr

ABSTRACT
Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.

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Related in: MedlinePlus

(A) Schematic illustration of microfluidic device and (B) its picture. SEM images of the (C–D) Y-junction and (E–F) detection chamber. All SEM images were shown in both top angle and tilted angle, and inserted scale bars are 500 μm.
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f2-sensors-12-10810: (A) Schematic illustration of microfluidic device and (B) its picture. SEM images of the (C–D) Y-junction and (E–F) detection chamber. All SEM images were shown in both top angle and tilted angle, and inserted scale bars are 500 μm.

Mentions: In this device, Y shaped inlet channels with backflow-prevented microstructure and detection chambers were designed for an efficient immunoassay as shown in Figure 2. The key features of microstructures in microfluidic device were successfully replicated using the microinjection molding, and they were confirmed through top and tilted scanning electron microscopy (SEM, Hitachi S4800, Ibaraki, Japan) images as shown in Figure 2(C–F). Due to the height differences between inlet channels and main channels (Figure 2(C,D)), this could prevent the backflow of the solutions. This device is composed of three detection chambers with 1 mm in diameter for the further immobilization of GBP-H1a fusion protein as shown in Figure 2, and these chambers may reduce the errors during analysis by averaging signals. All protuberant microstructures near to the microchannels were specially designed as welding lines. During the ultrasonic bonding process to bond the top and bottom of the microfluidic chip, these lines would be melted by concentrating the ultrasonic energies on the top of welding lines.


Development of a plastic-based microfluidic immunosensor chip for detection of H1N1 influenza.

Lee KG, Lee TJ, Jeong SW, Choi HW, Heo NS, Park JY, Park TJ, Lee SJ - Sensors (Basel) (2012)

(A) Schematic illustration of microfluidic device and (B) its picture. SEM images of the (C–D) Y-junction and (E–F) detection chamber. All SEM images were shown in both top angle and tilted angle, and inserted scale bars are 500 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472858&req=5

f2-sensors-12-10810: (A) Schematic illustration of microfluidic device and (B) its picture. SEM images of the (C–D) Y-junction and (E–F) detection chamber. All SEM images were shown in both top angle and tilted angle, and inserted scale bars are 500 μm.
Mentions: In this device, Y shaped inlet channels with backflow-prevented microstructure and detection chambers were designed for an efficient immunoassay as shown in Figure 2. The key features of microstructures in microfluidic device were successfully replicated using the microinjection molding, and they were confirmed through top and tilted scanning electron microscopy (SEM, Hitachi S4800, Ibaraki, Japan) images as shown in Figure 2(C–F). Due to the height differences between inlet channels and main channels (Figure 2(C,D)), this could prevent the backflow of the solutions. This device is composed of three detection chambers with 1 mm in diameter for the further immobilization of GBP-H1a fusion protein as shown in Figure 2, and these chambers may reduce the errors during analysis by averaging signals. All protuberant microstructures near to the microchannels were specially designed as welding lines. During the ultrasonic bonding process to bond the top and bottom of the microfluidic chip, these lines would be melted by concentrating the ultrasonic energies on the top of welding lines.

Bottom Line: A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique.For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip.This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.

View Article: PubMed Central - PubMed

Affiliation: Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea. kglee@nnfc.re.kr

ABSTRACT
Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.

Show MeSH
Related in: MedlinePlus