Limits...
Lab-on-a-chip pathogen sensors for food safety.

Yoon JY, Kim B - Sensors (Basel) (2012)

Bottom Line: Several different types of lab-on-a-chip biosensors, including immunoassay- and PCR-based, have been developed and tested for detecting foodborne pathogens.Their assay performance, including detection limit and assay time, are also summarized.Finally, the use of optical fibers or optical waveguide is discussed as a means to improve the portability and sensitivity of lab-on-a-chip pathogen sensors.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural and Biosystems Engineering, the University of Arizona, Tucson, AZ 85721, USA. jyyoon@email.arizona.edu

ABSTRACT
There have been a number of cases of foodborne illness among humans that are caused by pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, etc. The current practices to detect such pathogenic agents are cell culturing, immunoassays, or polymerase chain reactions (PCRs). These methods are essentially laboratory-based methods that are not at all real-time and thus unavailable for early-monitoring of such pathogens. They are also very difficult to implement in the field. Lab-on-a-chip biosensors, however, have a strong potential to be used in the field since they can be miniaturized and automated; they are also potentially fast and very sensitive. These lab-on-a-chip biosensors can detect pathogens in farms, packaging/processing facilities, delivery/distribution systems, and at the consumer level. There are still several issues to be resolved before applying these lab-on-a-chip sensors to field applications, including the pre-treatment of a sample, proper storage of reagents, full integration into a battery-powered system, and demonstration of very high sensitivity, which are addressed in this review article. Several different types of lab-on-a-chip biosensors, including immunoassay- and PCR-based, have been developed and tested for detecting foodborne pathogens. Their assay performance, including detection limit and assay time, are also summarized. Finally, the use of optical fibers or optical waveguide is discussed as a means to improve the portability and sensitivity of lab-on-a-chip pathogen sensors.

Show MeSH

Related in: MedlinePlus

A lab-on-a-chip contains a network of channels and wells. Reprinted from [12] with permission © Am erican Chemical Society.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3472853&req=5

f1-sensors-12-10713: A lab-on-a-chip contains a network of channels and wells. Reprinted from [12] with permission © Am erican Chemical Society.

Mentions: Lab-on-a-chip has recently been suggested as a perfect medium for portable and real-time medical diagnostics. Surely, there is no reason why we cannot use lab-on-a-chip for detecting foodborne pathogens. Lab-on-a-chip is a device that integrates several laboratory functions onto one small platform, typically only millimeters or centimeters in size. A lab-on-a-chip normally involves the handling of very small fluid volumes; this introduces the area of “microfluidics” that deals with the behavior, precise control, and manipulation of fluids that are constrained to a small, sub-millimeter scale. Lab-on-a-chip technology utilizes a network of channels and wells that are etched onto glass, silicon, or polymer chips to build mini-laboratories (Figure 1). Pressure or electrokinetic forces move small volumes in a finely controlled manner through the channels. Lab-on-a-chip enables sample handling, mixing, dilution, electrophoresis, staining, and detection on a single integrated system. The main advantages of lab-on-a-chip are: ease-of-use, speed of analysis, low sample and reagent consumption, and high reproducibility due to standardization and automation. In summary, the lab-on-a-chip-based biosensor is a perfect medium to make portable and real-time biosensing of foodborne pathogens possible.


Lab-on-a-chip pathogen sensors for food safety.

Yoon JY, Kim B - Sensors (Basel) (2012)

A lab-on-a-chip contains a network of channels and wells. Reprinted from [12] with permission © Am erican Chemical Society.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472853&req=5

f1-sensors-12-10713: A lab-on-a-chip contains a network of channels and wells. Reprinted from [12] with permission © Am erican Chemical Society.
Mentions: Lab-on-a-chip has recently been suggested as a perfect medium for portable and real-time medical diagnostics. Surely, there is no reason why we cannot use lab-on-a-chip for detecting foodborne pathogens. Lab-on-a-chip is a device that integrates several laboratory functions onto one small platform, typically only millimeters or centimeters in size. A lab-on-a-chip normally involves the handling of very small fluid volumes; this introduces the area of “microfluidics” that deals with the behavior, precise control, and manipulation of fluids that are constrained to a small, sub-millimeter scale. Lab-on-a-chip technology utilizes a network of channels and wells that are etched onto glass, silicon, or polymer chips to build mini-laboratories (Figure 1). Pressure or electrokinetic forces move small volumes in a finely controlled manner through the channels. Lab-on-a-chip enables sample handling, mixing, dilution, electrophoresis, staining, and detection on a single integrated system. The main advantages of lab-on-a-chip are: ease-of-use, speed of analysis, low sample and reagent consumption, and high reproducibility due to standardization and automation. In summary, the lab-on-a-chip-based biosensor is a perfect medium to make portable and real-time biosensing of foodborne pathogens possible.

Bottom Line: Several different types of lab-on-a-chip biosensors, including immunoassay- and PCR-based, have been developed and tested for detecting foodborne pathogens.Their assay performance, including detection limit and assay time, are also summarized.Finally, the use of optical fibers or optical waveguide is discussed as a means to improve the portability and sensitivity of lab-on-a-chip pathogen sensors.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural and Biosystems Engineering, the University of Arizona, Tucson, AZ 85721, USA. jyyoon@email.arizona.edu

ABSTRACT
There have been a number of cases of foodborne illness among humans that are caused by pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, etc. The current practices to detect such pathogenic agents are cell culturing, immunoassays, or polymerase chain reactions (PCRs). These methods are essentially laboratory-based methods that are not at all real-time and thus unavailable for early-monitoring of such pathogens. They are also very difficult to implement in the field. Lab-on-a-chip biosensors, however, have a strong potential to be used in the field since they can be miniaturized and automated; they are also potentially fast and very sensitive. These lab-on-a-chip biosensors can detect pathogens in farms, packaging/processing facilities, delivery/distribution systems, and at the consumer level. There are still several issues to be resolved before applying these lab-on-a-chip sensors to field applications, including the pre-treatment of a sample, proper storage of reagents, full integration into a battery-powered system, and demonstration of very high sensitivity, which are addressed in this review article. Several different types of lab-on-a-chip biosensors, including immunoassay- and PCR-based, have been developed and tested for detecting foodborne pathogens. Their assay performance, including detection limit and assay time, are also summarized. Finally, the use of optical fibers or optical waveguide is discussed as a means to improve the portability and sensitivity of lab-on-a-chip pathogen sensors.

Show MeSH
Related in: MedlinePlus