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Serum autofluorescence, a potential serum marker for the diagnosis of liver fibrosis in rats.

Zhan YT, Li L, Weng J, Song X, Yang SQ, An W - Int J Mol Sci (2012)

Bottom Line: The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves.Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis.ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; E-Mail: dadi-121@163.com.

ABSTRACT
Fluctuations in serum autofluorescence (AF) intensity have recently been widely used as markers of certain diseases such as cancer. To determine the diagnostic value of serum AF intensity for liver fibrosis in rats, we induced liver fibrosis by subcutaneous injection of carbon tetrachloride into rats. The rat serum AF intensities were detected at the excitation wavelength of 337 nm and the emission wavelength of 512 nm. The degree of liver fibrosis was evaluated by Van Gieson's staining. The relationship between serum AF intensity and the degree of liver fibrosis was analyzed by Spearman and Pearson Correlation. The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves. Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis. However, there was no significant difference in serum AF intensity between fibrotic and control rats at four week post induction. Furthermore, serum AF intensity correlated positively with the severity of the degree of hepatic fibrosis. ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis. Therefore, it may potentially be developed as a novel diagnostic tool for hepatic fibrosis.

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Related in: MedlinePlus

Relationship between serum autofluorescence (AF) intensity and stage of liver fibrosis. A steady gradual increase in serum AF intensity was observed with increasing severity of liver fibrosis (p < 0.01).
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Related In: Results  -  Collection

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f2-ijms-13-12130: Relationship between serum autofluorescence (AF) intensity and stage of liver fibrosis. A steady gradual increase in serum AF intensity was observed with increasing severity of liver fibrosis (p < 0.01).

Mentions: Figure 2 shows that the serum AF intensity increased gradually with the progression of hepatic fibrosis, and correlated positively with hepatic fibrosis (r = 0.604, p < 0.01).


Serum autofluorescence, a potential serum marker for the diagnosis of liver fibrosis in rats.

Zhan YT, Li L, Weng J, Song X, Yang SQ, An W - Int J Mol Sci (2012)

Relationship between serum autofluorescence (AF) intensity and stage of liver fibrosis. A steady gradual increase in serum AF intensity was observed with increasing severity of liver fibrosis (p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472797&req=5

f2-ijms-13-12130: Relationship between serum autofluorescence (AF) intensity and stage of liver fibrosis. A steady gradual increase in serum AF intensity was observed with increasing severity of liver fibrosis (p < 0.01).
Mentions: Figure 2 shows that the serum AF intensity increased gradually with the progression of hepatic fibrosis, and correlated positively with hepatic fibrosis (r = 0.604, p < 0.01).

Bottom Line: The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves.Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis.ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; E-Mail: dadi-121@163.com.

ABSTRACT
Fluctuations in serum autofluorescence (AF) intensity have recently been widely used as markers of certain diseases such as cancer. To determine the diagnostic value of serum AF intensity for liver fibrosis in rats, we induced liver fibrosis by subcutaneous injection of carbon tetrachloride into rats. The rat serum AF intensities were detected at the excitation wavelength of 337 nm and the emission wavelength of 512 nm. The degree of liver fibrosis was evaluated by Van Gieson's staining. The relationship between serum AF intensity and the degree of liver fibrosis was analyzed by Spearman and Pearson Correlation. The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves. Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis. However, there was no significant difference in serum AF intensity between fibrotic and control rats at four week post induction. Furthermore, serum AF intensity correlated positively with the severity of the degree of hepatic fibrosis. ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis. Therefore, it may potentially be developed as a novel diagnostic tool for hepatic fibrosis.

Show MeSH
Related in: MedlinePlus