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Serum autofluorescence, a potential serum marker for the diagnosis of liver fibrosis in rats.

Zhan YT, Li L, Weng J, Song X, Yang SQ, An W - Int J Mol Sci (2012)

Bottom Line: The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves.Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis.ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; E-Mail: dadi-121@163.com.

ABSTRACT
Fluctuations in serum autofluorescence (AF) intensity have recently been widely used as markers of certain diseases such as cancer. To determine the diagnostic value of serum AF intensity for liver fibrosis in rats, we induced liver fibrosis by subcutaneous injection of carbon tetrachloride into rats. The rat serum AF intensities were detected at the excitation wavelength of 337 nm and the emission wavelength of 512 nm. The degree of liver fibrosis was evaluated by Van Gieson's staining. The relationship between serum AF intensity and the degree of liver fibrosis was analyzed by Spearman and Pearson Correlation. The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves. Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis. However, there was no significant difference in serum AF intensity between fibrotic and control rats at four week post induction. Furthermore, serum AF intensity correlated positively with the severity of the degree of hepatic fibrosis. ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis. Therefore, it may potentially be developed as a novel diagnostic tool for hepatic fibrosis.

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Related in: MedlinePlus

Pathological changes in liver at different fibrotic stages. (A) Control group rats, thin fibers were seen around blood vessels or bile ducts; (B) fibrotic model group rats at the 4th week, thin collagen fibers extended into the fatty degeneration area; (C) model group rats at the 8th week, the hepatic lobule is enveloped incompletely by thin collagen fibers; (D) model group rats at the 12th week, large amount of fibrous connective tissue was observed, forming typical pseudolobules.
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f1-ijms-13-12130: Pathological changes in liver at different fibrotic stages. (A) Control group rats, thin fibers were seen around blood vessels or bile ducts; (B) fibrotic model group rats at the 4th week, thin collagen fibers extended into the fatty degeneration area; (C) model group rats at the 8th week, the hepatic lobule is enveloped incompletely by thin collagen fibers; (D) model group rats at the 12th week, large amount of fibrous connective tissue was observed, forming typical pseudolobules.

Mentions: Van Gieson’s staining clearly showed that little collagen was distributed around the blood vessel wall or bile duct wall in livers of control rats (Figure 1A). Thin collagen fibers were observed in liver rats with fibrosis starting from the 4th week (Figure 1B). At the 8th week, the hepatic lobule was incompletely enveloped by thin collagen fibers (Figure 1C). The liver damage progressed further at the 12th week, showing a larger accumulation of fibrous connective tissue and the formation of typical pseudolobules (Figure 1D).


Serum autofluorescence, a potential serum marker for the diagnosis of liver fibrosis in rats.

Zhan YT, Li L, Weng J, Song X, Yang SQ, An W - Int J Mol Sci (2012)

Pathological changes in liver at different fibrotic stages. (A) Control group rats, thin fibers were seen around blood vessels or bile ducts; (B) fibrotic model group rats at the 4th week, thin collagen fibers extended into the fatty degeneration area; (C) model group rats at the 8th week, the hepatic lobule is enveloped incompletely by thin collagen fibers; (D) model group rats at the 12th week, large amount of fibrous connective tissue was observed, forming typical pseudolobules.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472797&req=5

f1-ijms-13-12130: Pathological changes in liver at different fibrotic stages. (A) Control group rats, thin fibers were seen around blood vessels or bile ducts; (B) fibrotic model group rats at the 4th week, thin collagen fibers extended into the fatty degeneration area; (C) model group rats at the 8th week, the hepatic lobule is enveloped incompletely by thin collagen fibers; (D) model group rats at the 12th week, large amount of fibrous connective tissue was observed, forming typical pseudolobules.
Mentions: Van Gieson’s staining clearly showed that little collagen was distributed around the blood vessel wall or bile duct wall in livers of control rats (Figure 1A). Thin collagen fibers were observed in liver rats with fibrosis starting from the 4th week (Figure 1B). At the 8th week, the hepatic lobule was incompletely enveloped by thin collagen fibers (Figure 1C). The liver damage progressed further at the 12th week, showing a larger accumulation of fibrous connective tissue and the formation of typical pseudolobules (Figure 1D).

Bottom Line: The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves.Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis.ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; E-Mail: dadi-121@163.com.

ABSTRACT
Fluctuations in serum autofluorescence (AF) intensity have recently been widely used as markers of certain diseases such as cancer. To determine the diagnostic value of serum AF intensity for liver fibrosis in rats, we induced liver fibrosis by subcutaneous injection of carbon tetrachloride into rats. The rat serum AF intensities were detected at the excitation wavelength of 337 nm and the emission wavelength of 512 nm. The degree of liver fibrosis was evaluated by Van Gieson's staining. The relationship between serum AF intensity and the degree of liver fibrosis was analyzed by Spearman and Pearson Correlation. The diagnostic sensitivity and specificity of the serum AF was determined by analyzing the receiver operating characteristic (ROC) curves. Our results show that the serum AF intensity in the rat liver fibrosis model increased when compared with control rats eight weeks and twelve weeks post induction of liver fibrosis. However, there was no significant difference in serum AF intensity between fibrotic and control rats at four week post induction. Furthermore, serum AF intensity correlated positively with the severity of the degree of hepatic fibrosis. ROC analysis further suggested that serum AF intensity is a valid marker for staging fibrosis. Therefore, it may potentially be developed as a novel diagnostic tool for hepatic fibrosis.

Show MeSH
Related in: MedlinePlus