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Gelam honey scavenges peroxynitrite during the immune response.

Kassim M, Mansor M, Suhaimi A, Ong G, Yusoff KM - Int J Mol Sci (2012)

Bottom Line: Gelam honey significantly improved the viability of LPS/IFN-γ-treated RAW 264.7 cells and inhibited nitric oxide production-similar to the effects observed with an inhibitor of inducible nitric oxide synthase (1400W).Honey inhibited peroxynitrite synthesis in LPS-treated rats.Thus, honey may attenuate inflammatory responses that lead to cell damage and death, suggesting its therapeutic uses for several inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: marzida@gmail.com (M.M.); gracieo@um.edu.my (G.O.).

ABSTRACT
Monocytes and macrophages are part of the first-line defense against bacterial, fungal, and viral infections during host immune responses; they express high levels of proinflammatory cytokines and cytotoxic molecules, including nitric oxide, reactive oxygen species, and their reaction product peroxynitrite. Peroxynitrite is a short-lived oxidant and a potent inducer of cell death. Honey, in addition to its well-known sweetening properties, is a natural antioxidant that has been used since ancient times in traditional medicine. We examined the ability of Gelam honey, derived from the Gelam tree (Melaleuca spp.), to scavenge peroxynitrite during immune responses mounted in the murine macrophage cell line RAW 264.7 when stimulated with lipopolysaccharide/interferon-γ (LPS/IFN-γ) and in LPS-treated rats. Gelam honey significantly improved the viability of LPS/IFN-γ-treated RAW 264.7 cells and inhibited nitric oxide production-similar to the effects observed with an inhibitor of inducible nitric oxide synthase (1400W). Furthermore, honey, but not 1400W, inhibited peroxynitrite production from the synthetic substrate 3-morpholinosydnonimine (SIN-1) and prevented the peroxynitrite-mediated conversion of dihydrorhodamine 123 to its fluorescent oxidation product rhodamine 123. Honey inhibited peroxynitrite synthesis in LPS-treated rats. Thus, honey may attenuate inflammatory responses that lead to cell damage and death, suggesting its therapeutic uses for several inflammatory disorders.

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Related in: MedlinePlus

Cytoprotective effect of honey against LPS/IFN-γ-induced cytotoxicity. RAW 264.7 cells were incubated with either 1 μg/mL LPS and 35 ng/mL IFN-γ (A) or 3 μg/mL LPS and 35 ng/mL IFN-γ (B), and with various concentrations of honey or 100 μM of the iNOS inhibitor 1400W. The negative control was completely untreated (control), and the positive control was treated only with LPS/IFN-γ (LPS). After 24 h incubation, cell viability was determined using an MTT assay. *** p < 0.001 and ** p < 0.003 indicate significant differences compared with the LPS/IFN-γ group.
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f2-ijms-13-12113: Cytoprotective effect of honey against LPS/IFN-γ-induced cytotoxicity. RAW 264.7 cells were incubated with either 1 μg/mL LPS and 35 ng/mL IFN-γ (A) or 3 μg/mL LPS and 35 ng/mL IFN-γ (B), and with various concentrations of honey or 100 μM of the iNOS inhibitor 1400W. The negative control was completely untreated (control), and the positive control was treated only with LPS/IFN-γ (LPS). After 24 h incubation, cell viability was determined using an MTT assay. *** p < 0.001 and ** p < 0.003 indicate significant differences compared with the LPS/IFN-γ group.

Mentions: In contrast, as shown in Figure 2A, pretreatment with honey had a protective effect on LPS/IFN-γ-stimulated cells, significantly increasing their viability to >76% (p < 0.03), whereas the viability of cells pretreated with 1400W was >90% (p < 0.001). Increasing the LPS concentration to 3 μg/mL significantly reduced the viability of the untreated cells to <50% of the control value, whereas the addition of honey increased viability to >69% (p < 0.001), and the addition of 1400W resulted in >79% viability (p < 0.001) (Figure 2B).


Gelam honey scavenges peroxynitrite during the immune response.

Kassim M, Mansor M, Suhaimi A, Ong G, Yusoff KM - Int J Mol Sci (2012)

Cytoprotective effect of honey against LPS/IFN-γ-induced cytotoxicity. RAW 264.7 cells were incubated with either 1 μg/mL LPS and 35 ng/mL IFN-γ (A) or 3 μg/mL LPS and 35 ng/mL IFN-γ (B), and with various concentrations of honey or 100 μM of the iNOS inhibitor 1400W. The negative control was completely untreated (control), and the positive control was treated only with LPS/IFN-γ (LPS). After 24 h incubation, cell viability was determined using an MTT assay. *** p < 0.001 and ** p < 0.003 indicate significant differences compared with the LPS/IFN-γ group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472796&req=5

f2-ijms-13-12113: Cytoprotective effect of honey against LPS/IFN-γ-induced cytotoxicity. RAW 264.7 cells were incubated with either 1 μg/mL LPS and 35 ng/mL IFN-γ (A) or 3 μg/mL LPS and 35 ng/mL IFN-γ (B), and with various concentrations of honey or 100 μM of the iNOS inhibitor 1400W. The negative control was completely untreated (control), and the positive control was treated only with LPS/IFN-γ (LPS). After 24 h incubation, cell viability was determined using an MTT assay. *** p < 0.001 and ** p < 0.003 indicate significant differences compared with the LPS/IFN-γ group.
Mentions: In contrast, as shown in Figure 2A, pretreatment with honey had a protective effect on LPS/IFN-γ-stimulated cells, significantly increasing their viability to >76% (p < 0.03), whereas the viability of cells pretreated with 1400W was >90% (p < 0.001). Increasing the LPS concentration to 3 μg/mL significantly reduced the viability of the untreated cells to <50% of the control value, whereas the addition of honey increased viability to >69% (p < 0.001), and the addition of 1400W resulted in >79% viability (p < 0.001) (Figure 2B).

Bottom Line: Gelam honey significantly improved the viability of LPS/IFN-γ-treated RAW 264.7 cells and inhibited nitric oxide production-similar to the effects observed with an inhibitor of inducible nitric oxide synthase (1400W).Honey inhibited peroxynitrite synthesis in LPS-treated rats.Thus, honey may attenuate inflammatory responses that lead to cell damage and death, suggesting its therapeutic uses for several inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: marzida@gmail.com (M.M.); gracieo@um.edu.my (G.O.).

ABSTRACT
Monocytes and macrophages are part of the first-line defense against bacterial, fungal, and viral infections during host immune responses; they express high levels of proinflammatory cytokines and cytotoxic molecules, including nitric oxide, reactive oxygen species, and their reaction product peroxynitrite. Peroxynitrite is a short-lived oxidant and a potent inducer of cell death. Honey, in addition to its well-known sweetening properties, is a natural antioxidant that has been used since ancient times in traditional medicine. We examined the ability of Gelam honey, derived from the Gelam tree (Melaleuca spp.), to scavenge peroxynitrite during immune responses mounted in the murine macrophage cell line RAW 264.7 when stimulated with lipopolysaccharide/interferon-γ (LPS/IFN-γ) and in LPS-treated rats. Gelam honey significantly improved the viability of LPS/IFN-γ-treated RAW 264.7 cells and inhibited nitric oxide production-similar to the effects observed with an inhibitor of inducible nitric oxide synthase (1400W). Furthermore, honey, but not 1400W, inhibited peroxynitrite production from the synthetic substrate 3-morpholinosydnonimine (SIN-1) and prevented the peroxynitrite-mediated conversion of dihydrorhodamine 123 to its fluorescent oxidation product rhodamine 123. Honey inhibited peroxynitrite synthesis in LPS-treated rats. Thus, honey may attenuate inflammatory responses that lead to cell damage and death, suggesting its therapeutic uses for several inflammatory disorders.

Show MeSH
Related in: MedlinePlus