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Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels.

Yin RX, Wu DF, Aung LH, Yan TT, Cao XL, Long XJ, Miao L, Liu WY, Zhang L, Li M - Int J Mol Sci (2012)

Bottom Line: Genotyping of ATP-binding cassette transporter A1 (ABCA-1) V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) AvaII hepatic lipase gene (LIPC) -250G > A, endothelial lipase gene (LIPG) 584C > T, methylenetetrahydrofolate reductase (MTHFR) 677C > T, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein (MYLIP) rs3757354, proprotein convertase subtilisin-like kexin type 9 (PCSK9) E670G, peroxisome proliferator-activated receptor delta (PPARD) +294T > C, and Scavenger receptor class B type 1 (SCARB1) rs5888 was performed in 978 normal weight and 751 overweight/obese subjects.The interactions were detected by factorial regression analysis.The differences in blood pressure levels between normal weight and overweight/obese subjects might partly result from different interactions of several SNPs and overweight/obesity.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi, China; E-Mails: wulove26@tom.com (D.-F.W.); lifeinnivana@hotmail.com (L.H.H.A); yan083199@163.com (T.-T.Y.); ma101996@gmail.com (X.-L.C.); longxingjiang2012@163.com (X.-J.L.).

ABSTRACT
Little is known about the interactions of single nucleotide polymorphisms (SNPs) and overweight/obesity on blood pressure levels. The present study was undertaken to detect 10 lipid-related gene SNPs and their interactions with overweight/obesity on blood pressure levels. Genotyping of ATP-binding cassette transporter A1 (ABCA-1) V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) AvaII hepatic lipase gene (LIPC) -250G > A, endothelial lipase gene (LIPG) 584C > T, methylenetetrahydrofolate reductase (MTHFR) 677C > T, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein (MYLIP) rs3757354, proprotein convertase subtilisin-like kexin type 9 (PCSK9) E670G, peroxisome proliferator-activated receptor delta (PPARD) +294T > C, and Scavenger receptor class B type 1 (SCARB1) rs5888 was performed in 978 normal weight and 751 overweight/obese subjects. The interactions were detected by factorial regression analysis. The genotypes of ACAT-1 AC, LIPC GA and AA, and SCARB1 TT; LDL-R A-A- and LIPC GA; and SCARB1 TT were interacted with overweight/obesity to increase systolic, diastolic blood pressure (SBP, DBP) and pulse pressure (PP) levels; respectively. The genotypes of ACAT-1 CC; ACAT-1 AA and CC were interacted with overweight/obesity to decrease SBP, PP levels (p < 0.01-0.001); respectively. The differences in blood pressure levels between normal weight and overweight/obese subjects might partly result from different interactions of several SNPs and overweight/obesity.

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Related in: MedlinePlus

Genotyping of 10 lipid-related gene polymorphisms by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). ABCA1, ATP-binding cassette transporter A1. Lane M, 100 bp marker ladder; lanes 1 and 2, GG genotype (525 bp); lanes 3 and 4, GA genotype (525-, 302- and 223-bp); and lanes 5 and 6, AA genotype (302- and 223-bp); ACAT-1, acyl-CoA:cholesterol acyltransferase-1. Lane M, 100 bp marker ladder; lanes 1–3, AA genotype (389 bp); lanes 4 and 5, AC genotype (389-, 279- and 110-bp); and lanes 6 and 7, CC genotype (279- and 110-bp); LDL-R, low density lipoprotein receptor. Lane M, 50 bp marker ladder; lanes 1–6, A+A+ genotype (141- and 87-bp); lanes 7–12, A-A+ genotype (228-, 141- and 87-bp); and lanes 13–15, A-A- genotype (228-bp); LIPC, hepatic lipase gene. Lane M, 100 bp marker ladder; lanes 1–3, GG genotype (411 bp); lane 4, GA genotype (411-, 301- and 110-bp); and lanes 5–7, AA genotype (301- and 110-bp); LIPG, endothelial lipase gene. Lane M, 50 bp marker ladder; lane 1, the PCR product of the sample (254 bp); lanes 2 and 3, CC genotype (254 bp); lanes 4 and 5, CT genotype (254-, 217- and 37-bp); and lanes 6 and 7, TT genotype (217- and 37-bp). The 37 bp fragment was invisible in the gel owing to its fast migration speed; MTHFR, methylenetetrahydrofolate reductase. Lane M, 50 bp marker ladder; lanes 1–3, CC genotype (245 bp); lanes 4–6, CT genotype (245-, 173- and 72-bp); and lanes 7 and 8, TT genotype (173- and 72-bp); MYLIP, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein. Lane M, 100 bp marker ladder; lanes 1 and 2, AA genotype (387 bp); lanes 3 and 4, AG genotype (387-, 306- and 81-bp); and lanes 5 and 6, GG genotype (306- and 81-bp); PCSK9, proprotein convertase subtilisin-like kexin type 9. Lane M, 100 bp marker ladder; lanes 1 and 2, PCR products of the samples (440 bp); lanes 3 and 4, AG genotype (440-, 290- and 150-bp); and lanes 5 and 6, AA genotype (290- and 150-bp). The GG homozygous of the PCSK9 E670G was not detected in our study population; PPARD, peroxisome proliferator-activated receptor delta. Lane M, 100 bp marker ladder; lanes 1 and 2, TT genotype (269 bp); lanes 3–5, TC genotype (269-, 167- and 102-bp); and lanes 6 and 7, CC genotype (167- and 102-bp); SCARB1, Scavenger receptor class B type 1. Lane M, 50 bp marker ladder; lanes 1 and 2, TT genotype (218 bp); lanes 3 and 4, CT genotype (218-, 187- and 31-bp); and lanes 5 and 6, CC genotype (187- and 31-bp). The 31 bp fragment was invisible in the gel owing to its fast migration speed.
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f1-ijms-13-12062: Genotyping of 10 lipid-related gene polymorphisms by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). ABCA1, ATP-binding cassette transporter A1. Lane M, 100 bp marker ladder; lanes 1 and 2, GG genotype (525 bp); lanes 3 and 4, GA genotype (525-, 302- and 223-bp); and lanes 5 and 6, AA genotype (302- and 223-bp); ACAT-1, acyl-CoA:cholesterol acyltransferase-1. Lane M, 100 bp marker ladder; lanes 1–3, AA genotype (389 bp); lanes 4 and 5, AC genotype (389-, 279- and 110-bp); and lanes 6 and 7, CC genotype (279- and 110-bp); LDL-R, low density lipoprotein receptor. Lane M, 50 bp marker ladder; lanes 1–6, A+A+ genotype (141- and 87-bp); lanes 7–12, A-A+ genotype (228-, 141- and 87-bp); and lanes 13–15, A-A- genotype (228-bp); LIPC, hepatic lipase gene. Lane M, 100 bp marker ladder; lanes 1–3, GG genotype (411 bp); lane 4, GA genotype (411-, 301- and 110-bp); and lanes 5–7, AA genotype (301- and 110-bp); LIPG, endothelial lipase gene. Lane M, 50 bp marker ladder; lane 1, the PCR product of the sample (254 bp); lanes 2 and 3, CC genotype (254 bp); lanes 4 and 5, CT genotype (254-, 217- and 37-bp); and lanes 6 and 7, TT genotype (217- and 37-bp). The 37 bp fragment was invisible in the gel owing to its fast migration speed; MTHFR, methylenetetrahydrofolate reductase. Lane M, 50 bp marker ladder; lanes 1–3, CC genotype (245 bp); lanes 4–6, CT genotype (245-, 173- and 72-bp); and lanes 7 and 8, TT genotype (173- and 72-bp); MYLIP, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein. Lane M, 100 bp marker ladder; lanes 1 and 2, AA genotype (387 bp); lanes 3 and 4, AG genotype (387-, 306- and 81-bp); and lanes 5 and 6, GG genotype (306- and 81-bp); PCSK9, proprotein convertase subtilisin-like kexin type 9. Lane M, 100 bp marker ladder; lanes 1 and 2, PCR products of the samples (440 bp); lanes 3 and 4, AG genotype (440-, 290- and 150-bp); and lanes 5 and 6, AA genotype (290- and 150-bp). The GG homozygous of the PCSK9 E670G was not detected in our study population; PPARD, peroxisome proliferator-activated receptor delta. Lane M, 100 bp marker ladder; lanes 1 and 2, TT genotype (269 bp); lanes 3–5, TC genotype (269-, 167- and 102-bp); and lanes 6 and 7, CC genotype (167- and 102-bp); SCARB1, Scavenger receptor class B type 1. Lane M, 50 bp marker ladder; lanes 1 and 2, TT genotype (218 bp); lanes 3 and 4, CT genotype (218-, 187- and 31-bp); and lanes 5 and 6, CC genotype (187- and 31-bp). The 31 bp fragment was invisible in the gel owing to its fast migration speed.

Mentions: The polymerase chain reaction (PCR) products of ATP-binding cassette transporter A1 (ABCA-1) V825I (rs2066715), acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) AvaII, hepatic lipase gene (LIPC) −250G > A (rs2070895), endothelial lipase gene (LIPG) 584C > T (rs2000813), methylenetetrahydrofolate reductase (MTHFR) 677C > T (rs1801133), the E3 ubiquitin ligase myosin regulatory light chain-interacting protein (MYLIP, also known as IDOL) rs3757354, proprotein convertase subtilisin-like kexin type 9 (PCSK9) E670G (rs505151), peroxisome proliferator-activated receptor delta (PPARD) +294T > C (rs2016520) and Scavenger receptor class B type 1 (SCARB1) rs5888 were 525-, 389-, 228-, 411-, 254-, 254-, 387-, 440-, 269- and 218-bp nucleotide sequences; respectively. The genotypes of the 10 SNPs were shown in Figure 1.


Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels.

Yin RX, Wu DF, Aung LH, Yan TT, Cao XL, Long XJ, Miao L, Liu WY, Zhang L, Li M - Int J Mol Sci (2012)

Genotyping of 10 lipid-related gene polymorphisms by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). ABCA1, ATP-binding cassette transporter A1. Lane M, 100 bp marker ladder; lanes 1 and 2, GG genotype (525 bp); lanes 3 and 4, GA genotype (525-, 302- and 223-bp); and lanes 5 and 6, AA genotype (302- and 223-bp); ACAT-1, acyl-CoA:cholesterol acyltransferase-1. Lane M, 100 bp marker ladder; lanes 1–3, AA genotype (389 bp); lanes 4 and 5, AC genotype (389-, 279- and 110-bp); and lanes 6 and 7, CC genotype (279- and 110-bp); LDL-R, low density lipoprotein receptor. Lane M, 50 bp marker ladder; lanes 1–6, A+A+ genotype (141- and 87-bp); lanes 7–12, A-A+ genotype (228-, 141- and 87-bp); and lanes 13–15, A-A- genotype (228-bp); LIPC, hepatic lipase gene. Lane M, 100 bp marker ladder; lanes 1–3, GG genotype (411 bp); lane 4, GA genotype (411-, 301- and 110-bp); and lanes 5–7, AA genotype (301- and 110-bp); LIPG, endothelial lipase gene. Lane M, 50 bp marker ladder; lane 1, the PCR product of the sample (254 bp); lanes 2 and 3, CC genotype (254 bp); lanes 4 and 5, CT genotype (254-, 217- and 37-bp); and lanes 6 and 7, TT genotype (217- and 37-bp). The 37 bp fragment was invisible in the gel owing to its fast migration speed; MTHFR, methylenetetrahydrofolate reductase. Lane M, 50 bp marker ladder; lanes 1–3, CC genotype (245 bp); lanes 4–6, CT genotype (245-, 173- and 72-bp); and lanes 7 and 8, TT genotype (173- and 72-bp); MYLIP, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein. Lane M, 100 bp marker ladder; lanes 1 and 2, AA genotype (387 bp); lanes 3 and 4, AG genotype (387-, 306- and 81-bp); and lanes 5 and 6, GG genotype (306- and 81-bp); PCSK9, proprotein convertase subtilisin-like kexin type 9. Lane M, 100 bp marker ladder; lanes 1 and 2, PCR products of the samples (440 bp); lanes 3 and 4, AG genotype (440-, 290- and 150-bp); and lanes 5 and 6, AA genotype (290- and 150-bp). The GG homozygous of the PCSK9 E670G was not detected in our study population; PPARD, peroxisome proliferator-activated receptor delta. Lane M, 100 bp marker ladder; lanes 1 and 2, TT genotype (269 bp); lanes 3–5, TC genotype (269-, 167- and 102-bp); and lanes 6 and 7, CC genotype (167- and 102-bp); SCARB1, Scavenger receptor class B type 1. Lane M, 50 bp marker ladder; lanes 1 and 2, TT genotype (218 bp); lanes 3 and 4, CT genotype (218-, 187- and 31-bp); and lanes 5 and 6, CC genotype (187- and 31-bp). The 31 bp fragment was invisible in the gel owing to its fast migration speed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472792&req=5

f1-ijms-13-12062: Genotyping of 10 lipid-related gene polymorphisms by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). ABCA1, ATP-binding cassette transporter A1. Lane M, 100 bp marker ladder; lanes 1 and 2, GG genotype (525 bp); lanes 3 and 4, GA genotype (525-, 302- and 223-bp); and lanes 5 and 6, AA genotype (302- and 223-bp); ACAT-1, acyl-CoA:cholesterol acyltransferase-1. Lane M, 100 bp marker ladder; lanes 1–3, AA genotype (389 bp); lanes 4 and 5, AC genotype (389-, 279- and 110-bp); and lanes 6 and 7, CC genotype (279- and 110-bp); LDL-R, low density lipoprotein receptor. Lane M, 50 bp marker ladder; lanes 1–6, A+A+ genotype (141- and 87-bp); lanes 7–12, A-A+ genotype (228-, 141- and 87-bp); and lanes 13–15, A-A- genotype (228-bp); LIPC, hepatic lipase gene. Lane M, 100 bp marker ladder; lanes 1–3, GG genotype (411 bp); lane 4, GA genotype (411-, 301- and 110-bp); and lanes 5–7, AA genotype (301- and 110-bp); LIPG, endothelial lipase gene. Lane M, 50 bp marker ladder; lane 1, the PCR product of the sample (254 bp); lanes 2 and 3, CC genotype (254 bp); lanes 4 and 5, CT genotype (254-, 217- and 37-bp); and lanes 6 and 7, TT genotype (217- and 37-bp). The 37 bp fragment was invisible in the gel owing to its fast migration speed; MTHFR, methylenetetrahydrofolate reductase. Lane M, 50 bp marker ladder; lanes 1–3, CC genotype (245 bp); lanes 4–6, CT genotype (245-, 173- and 72-bp); and lanes 7 and 8, TT genotype (173- and 72-bp); MYLIP, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein. Lane M, 100 bp marker ladder; lanes 1 and 2, AA genotype (387 bp); lanes 3 and 4, AG genotype (387-, 306- and 81-bp); and lanes 5 and 6, GG genotype (306- and 81-bp); PCSK9, proprotein convertase subtilisin-like kexin type 9. Lane M, 100 bp marker ladder; lanes 1 and 2, PCR products of the samples (440 bp); lanes 3 and 4, AG genotype (440-, 290- and 150-bp); and lanes 5 and 6, AA genotype (290- and 150-bp). The GG homozygous of the PCSK9 E670G was not detected in our study population; PPARD, peroxisome proliferator-activated receptor delta. Lane M, 100 bp marker ladder; lanes 1 and 2, TT genotype (269 bp); lanes 3–5, TC genotype (269-, 167- and 102-bp); and lanes 6 and 7, CC genotype (167- and 102-bp); SCARB1, Scavenger receptor class B type 1. Lane M, 50 bp marker ladder; lanes 1 and 2, TT genotype (218 bp); lanes 3 and 4, CT genotype (218-, 187- and 31-bp); and lanes 5 and 6, CC genotype (187- and 31-bp). The 31 bp fragment was invisible in the gel owing to its fast migration speed.
Mentions: The polymerase chain reaction (PCR) products of ATP-binding cassette transporter A1 (ABCA-1) V825I (rs2066715), acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) AvaII, hepatic lipase gene (LIPC) −250G > A (rs2070895), endothelial lipase gene (LIPG) 584C > T (rs2000813), methylenetetrahydrofolate reductase (MTHFR) 677C > T (rs1801133), the E3 ubiquitin ligase myosin regulatory light chain-interacting protein (MYLIP, also known as IDOL) rs3757354, proprotein convertase subtilisin-like kexin type 9 (PCSK9) E670G (rs505151), peroxisome proliferator-activated receptor delta (PPARD) +294T > C (rs2016520) and Scavenger receptor class B type 1 (SCARB1) rs5888 were 525-, 389-, 228-, 411-, 254-, 254-, 387-, 440-, 269- and 218-bp nucleotide sequences; respectively. The genotypes of the 10 SNPs were shown in Figure 1.

Bottom Line: Genotyping of ATP-binding cassette transporter A1 (ABCA-1) V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) AvaII hepatic lipase gene (LIPC) -250G > A, endothelial lipase gene (LIPG) 584C > T, methylenetetrahydrofolate reductase (MTHFR) 677C > T, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein (MYLIP) rs3757354, proprotein convertase subtilisin-like kexin type 9 (PCSK9) E670G, peroxisome proliferator-activated receptor delta (PPARD) +294T > C, and Scavenger receptor class B type 1 (SCARB1) rs5888 was performed in 978 normal weight and 751 overweight/obese subjects.The interactions were detected by factorial regression analysis.The differences in blood pressure levels between normal weight and overweight/obese subjects might partly result from different interactions of several SNPs and overweight/obesity.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi, China; E-Mails: wulove26@tom.com (D.-F.W.); lifeinnivana@hotmail.com (L.H.H.A); yan083199@163.com (T.-T.Y.); ma101996@gmail.com (X.-L.C.); longxingjiang2012@163.com (X.-J.L.).

ABSTRACT
Little is known about the interactions of single nucleotide polymorphisms (SNPs) and overweight/obesity on blood pressure levels. The present study was undertaken to detect 10 lipid-related gene SNPs and their interactions with overweight/obesity on blood pressure levels. Genotyping of ATP-binding cassette transporter A1 (ABCA-1) V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) AvaII hepatic lipase gene (LIPC) -250G > A, endothelial lipase gene (LIPG) 584C > T, methylenetetrahydrofolate reductase (MTHFR) 677C > T, the E3 ubiquitin ligase myosin regulatory light chain-interacting protein (MYLIP) rs3757354, proprotein convertase subtilisin-like kexin type 9 (PCSK9) E670G, peroxisome proliferator-activated receptor delta (PPARD) +294T > C, and Scavenger receptor class B type 1 (SCARB1) rs5888 was performed in 978 normal weight and 751 overweight/obese subjects. The interactions were detected by factorial regression analysis. The genotypes of ACAT-1 AC, LIPC GA and AA, and SCARB1 TT; LDL-R A-A- and LIPC GA; and SCARB1 TT were interacted with overweight/obesity to increase systolic, diastolic blood pressure (SBP, DBP) and pulse pressure (PP) levels; respectively. The genotypes of ACAT-1 CC; ACAT-1 AA and CC were interacted with overweight/obesity to decrease SBP, PP levels (p < 0.01-0.001); respectively. The differences in blood pressure levels between normal weight and overweight/obese subjects might partly result from different interactions of several SNPs and overweight/obesity.

Show MeSH
Related in: MedlinePlus