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Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.

Zaulet M, Gurau MR, Petrovan V, Buburuzan L - Int J Mol Sci (2012)

Bottom Line: One Romanian isolate (Rom3) was found in three of the four different investigated farms.The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus.The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania; E-Mails: zaulet_mihaela@yahoo.com (M.Z.); petrovan.vlad@gmail.com (V.P.).

ABSTRACT
Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

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Related in: MedlinePlus

The amino acid sequences of GP5 (201 amino acids) divided into signal peptide (1st aa to 32nd aa) and the mature chain (33rd aa to 201st aa). The mature chain contains two transmembrane elements (TM1—64th aa to 84th aa, TM2—109th aa to 129th aa).
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f4-ijms-13-12046: The amino acid sequences of GP5 (201 amino acids) divided into signal peptide (1st aa to 32nd aa) and the mature chain (33rd aa to 201st aa). The mature chain contains two transmembrane elements (TM1—64th aa to 84th aa, TM2—109th aa to 129th aa).

Mentions: The deduced amino acid sequences encoded by both ORF5 and ORF7 genes were aligned using CLUSTAL W program [9], resulting in a 201 amino acids alignment, corresponding to ORF5 gene (Figure 4) and a 128 amino acid alignment corresponding to ORF7 gene (Figure 5). A single exception can be noticed: the amino acid sequence for Rom22 isolate from Braila farm has an asparagine inserted at position 12 of the sequence.


Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.

Zaulet M, Gurau MR, Petrovan V, Buburuzan L - Int J Mol Sci (2012)

The amino acid sequences of GP5 (201 amino acids) divided into signal peptide (1st aa to 32nd aa) and the mature chain (33rd aa to 201st aa). The mature chain contains two transmembrane elements (TM1—64th aa to 84th aa, TM2—109th aa to 129th aa).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472791&req=5

f4-ijms-13-12046: The amino acid sequences of GP5 (201 amino acids) divided into signal peptide (1st aa to 32nd aa) and the mature chain (33rd aa to 201st aa). The mature chain contains two transmembrane elements (TM1—64th aa to 84th aa, TM2—109th aa to 129th aa).
Mentions: The deduced amino acid sequences encoded by both ORF5 and ORF7 genes were aligned using CLUSTAL W program [9], resulting in a 201 amino acids alignment, corresponding to ORF5 gene (Figure 4) and a 128 amino acid alignment corresponding to ORF7 gene (Figure 5). A single exception can be noticed: the amino acid sequence for Rom22 isolate from Braila farm has an asparagine inserted at position 12 of the sequence.

Bottom Line: One Romanian isolate (Rom3) was found in three of the four different investigated farms.The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus.The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania; E-Mails: zaulet_mihaela@yahoo.com (M.Z.); petrovan.vlad@gmail.com (V.P.).

ABSTRACT
Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

Show MeSH
Related in: MedlinePlus