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Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.

Zaulet M, Gurau MR, Petrovan V, Buburuzan L - Int J Mol Sci (2012)

Bottom Line: One Romanian isolate (Rom3) was found in three of the four different investigated farms.The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus.The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania; E-Mails: zaulet_mihaela@yahoo.com (M.Z.); petrovan.vlad@gmail.com (V.P.).

ABSTRACT
Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

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Related in: MedlinePlus

Maximum Likelihood tree based on ORF7 for the Romanian sequences, together with similar sequences from GenBank Database. The evolutionary distances were computed using the Tamura-Nei + G + I method. The bootstrap values adjacent to the main nodes represent the probabilities based on 1000 replicates.
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f3-ijms-13-12046: Maximum Likelihood tree based on ORF7 for the Romanian sequences, together with similar sequences from GenBank Database. The evolutionary distances were computed using the Tamura-Nei + G + I method. The bootstrap values adjacent to the main nodes represent the probabilities based on 1000 replicates.

Mentions: The phylogenetic tree obtained based on ORF7 sequences (Figure 3) shows the same topology as the tree based on ORF5 sequences. The division of the type 1 clade into three large subtypes 1, 2 and 3, isolates is also noticeable as suggested by other authors as well [10]. We realized that one sequence, DQ324708, is not included in the three subtypes described; all other sequences were included in the three subtypes.


Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.

Zaulet M, Gurau MR, Petrovan V, Buburuzan L - Int J Mol Sci (2012)

Maximum Likelihood tree based on ORF7 for the Romanian sequences, together with similar sequences from GenBank Database. The evolutionary distances were computed using the Tamura-Nei + G + I method. The bootstrap values adjacent to the main nodes represent the probabilities based on 1000 replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472791&req=5

f3-ijms-13-12046: Maximum Likelihood tree based on ORF7 for the Romanian sequences, together with similar sequences from GenBank Database. The evolutionary distances were computed using the Tamura-Nei + G + I method. The bootstrap values adjacent to the main nodes represent the probabilities based on 1000 replicates.
Mentions: The phylogenetic tree obtained based on ORF7 sequences (Figure 3) shows the same topology as the tree based on ORF5 sequences. The division of the type 1 clade into three large subtypes 1, 2 and 3, isolates is also noticeable as suggested by other authors as well [10]. We realized that one sequence, DQ324708, is not included in the three subtypes described; all other sequences were included in the three subtypes.

Bottom Line: One Romanian isolate (Rom3) was found in three of the four different investigated farms.The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus.The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania; E-Mails: zaulet_mihaela@yahoo.com (M.Z.); petrovan.vlad@gmail.com (V.P.).

ABSTRACT
Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

Show MeSH
Related in: MedlinePlus