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Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.

Zaulet M, Gurau MR, Petrovan V, Buburuzan L - Int J Mol Sci (2012)

Bottom Line: One Romanian isolate (Rom3) was found in three of the four different investigated farms.The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus.The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania; E-Mails: zaulet_mihaela@yahoo.com (M.Z.); petrovan.vlad@gmail.com (V.P.).

ABSTRACT
Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

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Related in: MedlinePlus

Political map of Romania showing the number of pigs from different areas.
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f1-ijms-13-12046: Political map of Romania showing the number of pigs from different areas.

Mentions: In order to investigate the PRRSV genetic variability in four pig farms in Romania, 605 samples were collected from different individuals. From the total amount of samples, 33 were positive for PRRSV by PCR. We were able to conduct the investigations on both ORF5 and ORF7 genes on 23 samples but for the other 10 samples due to the small quantity of initial tissue, we were only able to fulfill the investigations on the ORF5 gene (Table 1). In our study, we divided Romania into four different areas (Figure 1).


Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.

Zaulet M, Gurau MR, Petrovan V, Buburuzan L - Int J Mol Sci (2012)

Political map of Romania showing the number of pigs from different areas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472791&req=5

f1-ijms-13-12046: Political map of Romania showing the number of pigs from different areas.
Mentions: In order to investigate the PRRSV genetic variability in four pig farms in Romania, 605 samples were collected from different individuals. From the total amount of samples, 33 were positive for PRRSV by PCR. We were able to conduct the investigations on both ORF5 and ORF7 genes on 23 samples but for the other 10 samples due to the small quantity of initial tissue, we were only able to fulfill the investigations on the ORF5 gene (Table 1). In our study, we divided Romania into four different areas (Figure 1).

Bottom Line: One Romanian isolate (Rom3) was found in three of the four different investigated farms.The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus.The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania; E-Mails: zaulet_mihaela@yahoo.com (M.Z.); petrovan.vlad@gmail.com (V.P.).

ABSTRACT
Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.

Show MeSH
Related in: MedlinePlus