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Polystyrene attached Pt(IV)-azomethine, synthesis and immmobilization of glucose oxidase enzyme.

Sarı N, Antepli E, Nartop D, Yetim NK - Int J Mol Sci (2012)

Bottom Line: The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7.The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4.The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara 06500, Turkey; E-Mails: esinantpl@hotmail.com (E.A.); nurdankurnaz@gazi.edu.tr (N.K.Y.).

ABSTRACT
Modified polystyrene with Pt(IV)-azomethine (APS-Sch-Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

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Synthesis route of support for immobilization of glucose oxidase enzyme (GOx) enzyme.
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f5-ijms-13-11870: Synthesis route of support for immobilization of glucose oxidase enzyme (GOx) enzyme.

Mentions: To investigate to what extent the effect of coordination covalent bonds depends on enzyme immobilization, we prepared a polymeric support involving Pt(IV) ions. To prepare such a polymeric support, the (aminomethyl) polystyrene (APS) reacted with 2-hydroxybenzaldehyde (APS–Sch; Scheme 1A) and then the metal-containing complex was prepared using PtCl4 salts (APS–Sch–Pt), (Scheme 1B).


Polystyrene attached Pt(IV)-azomethine, synthesis and immmobilization of glucose oxidase enzyme.

Sarı N, Antepli E, Nartop D, Yetim NK - Int J Mol Sci (2012)

Synthesis route of support for immobilization of glucose oxidase enzyme (GOx) enzyme.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472780&req=5

f5-ijms-13-11870: Synthesis route of support for immobilization of glucose oxidase enzyme (GOx) enzyme.
Mentions: To investigate to what extent the effect of coordination covalent bonds depends on enzyme immobilization, we prepared a polymeric support involving Pt(IV) ions. To prepare such a polymeric support, the (aminomethyl) polystyrene (APS) reacted with 2-hydroxybenzaldehyde (APS–Sch; Scheme 1A) and then the metal-containing complex was prepared using PtCl4 salts (APS–Sch–Pt), (Scheme 1B).

Bottom Line: The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7.The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4.The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara 06500, Turkey; E-Mails: esinantpl@hotmail.com (E.A.); nurdankurnaz@gazi.edu.tr (N.K.Y.).

ABSTRACT
Modified polystyrene with Pt(IV)-azomethine (APS-Sch-Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

Show MeSH
Related in: MedlinePlus