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Polystyrene attached Pt(IV)-azomethine, synthesis and immmobilization of glucose oxidase enzyme.

Sarı N, Antepli E, Nartop D, Yetim NK - Int J Mol Sci (2012)

Bottom Line: The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7.The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4.The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara 06500, Turkey; E-Mails: esinantpl@hotmail.com (E.A.); nurdankurnaz@gazi.edu.tr (N.K.Y.).

ABSTRACT
Modified polystyrene with Pt(IV)-azomethine (APS-Sch-Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

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Effect of reuse of immobilized enzyme (A); Reuse image of activity of initial and fifteenth use of immobilized enzyme (B) at pH = 7 and t =70 °C.
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f3-ijms-13-11870: Effect of reuse of immobilized enzyme (A); Reuse image of activity of initial and fifteenth use of immobilized enzyme (B) at pH = 7 and t =70 °C.

Mentions: The storage stability of an enzyme is one of its most important characteristics. Enzymes often lose their activity during storage. Free and immobilized enzymes were stored in a dark bottle at +4 °C for 16 months (Figure 3) in a dry form. After 16 months, the free GOx and immobilized enzyme retained 95.26% and 96.76% of their original activity, respectively. High activitiy was found for the modified polymer. This result suggests that the modified polymer-support confers a higher conformational stability on the immobilized enzyme due to the formation of coordinate covalent bonds with the Pt(IV) atom (Figure 3).


Polystyrene attached Pt(IV)-azomethine, synthesis and immmobilization of glucose oxidase enzyme.

Sarı N, Antepli E, Nartop D, Yetim NK - Int J Mol Sci (2012)

Effect of reuse of immobilized enzyme (A); Reuse image of activity of initial and fifteenth use of immobilized enzyme (B) at pH = 7 and t =70 °C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472780&req=5

f3-ijms-13-11870: Effect of reuse of immobilized enzyme (A); Reuse image of activity of initial and fifteenth use of immobilized enzyme (B) at pH = 7 and t =70 °C.
Mentions: The storage stability of an enzyme is one of its most important characteristics. Enzymes often lose their activity during storage. Free and immobilized enzymes were stored in a dark bottle at +4 °C for 16 months (Figure 3) in a dry form. After 16 months, the free GOx and immobilized enzyme retained 95.26% and 96.76% of their original activity, respectively. High activitiy was found for the modified polymer. This result suggests that the modified polymer-support confers a higher conformational stability on the immobilized enzyme due to the formation of coordinate covalent bonds with the Pt(IV) atom (Figure 3).

Bottom Line: The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7.The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4.The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara 06500, Turkey; E-Mails: esinantpl@hotmail.com (E.A.); nurdankurnaz@gazi.edu.tr (N.K.Y.).

ABSTRACT
Modified polystyrene with Pt(IV)-azomethine (APS-Sch-Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

Show MeSH
Related in: MedlinePlus