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High yield of wax ester synthesized from cetyl alcohol and octanoic acid by lipozyme RMIM and Novozym 435.

Kuo CH, Chen HH, Chen JH, Liu YC, Shieh CJ - Int J Mol Sci (2012)

Bottom Line: Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1-5 h), reaction temperature (45-65 °C), substrate molar ratio (1-3:1), and enzyme amount (10%-50%) on the yield of cetyl octanoate.Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme(®) RMIM and Novozym(®) 435, respectively.Novozym(®) 435 proves to be a more efficient biocatalyst than Lipozyme(®) RMIM.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 402, Taiwan; E-Mail: jahomekuo@gmail.com.

ABSTRACT
Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme(®) RMIM (Rhizomucor miehei) and Novozym(®) 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1-5 h), reaction temperature (45-65 °C), substrate molar ratio (1-3:1), and enzyme amount (10%-50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme(®) RMIM and Novozym(®) 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym(®) 435 proves to be a more efficient biocatalyst than Lipozyme(®) RMIM.

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Related in: MedlinePlus

The relationship between predicted and experimental yield of cetyl octanoate for the two lipases tested: (a) Lipozyme® RMIM and (b) Novozym® 435.
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f1-ijms-13-11694: The relationship between predicted and experimental yield of cetyl octanoate for the two lipases tested: (a) Lipozyme® RMIM and (b) Novozym® 435.

Mentions: The plots of experimental values of the yield (%) versus those calculated from Equations 1 and 2 are shown in Figure 1. The predicted values from RSM models fit well with the experimental values with a high determination coefficient of 0.92 and 0.94 respectively for Lipozyme® RMIM and Novozym® 435.


High yield of wax ester synthesized from cetyl alcohol and octanoic acid by lipozyme RMIM and Novozym 435.

Kuo CH, Chen HH, Chen JH, Liu YC, Shieh CJ - Int J Mol Sci (2012)

The relationship between predicted and experimental yield of cetyl octanoate for the two lipases tested: (a) Lipozyme® RMIM and (b) Novozym® 435.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472770&req=5

f1-ijms-13-11694: The relationship between predicted and experimental yield of cetyl octanoate for the two lipases tested: (a) Lipozyme® RMIM and (b) Novozym® 435.
Mentions: The plots of experimental values of the yield (%) versus those calculated from Equations 1 and 2 are shown in Figure 1. The predicted values from RSM models fit well with the experimental values with a high determination coefficient of 0.92 and 0.94 respectively for Lipozyme® RMIM and Novozym® 435.

Bottom Line: Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1-5 h), reaction temperature (45-65 °C), substrate molar ratio (1-3:1), and enzyme amount (10%-50%) on the yield of cetyl octanoate.Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme(®) RMIM and Novozym(®) 435, respectively.Novozym(®) 435 proves to be a more efficient biocatalyst than Lipozyme(®) RMIM.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 402, Taiwan; E-Mail: jahomekuo@gmail.com.

ABSTRACT
Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme(®) RMIM (Rhizomucor miehei) and Novozym(®) 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1-5 h), reaction temperature (45-65 °C), substrate molar ratio (1-3:1), and enzyme amount (10%-50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme(®) RMIM and Novozym(®) 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym(®) 435 proves to be a more efficient biocatalyst than Lipozyme(®) RMIM.

Show MeSH
Related in: MedlinePlus