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Experimental aspects of colloidal interactions in mixed systems of liposome and inorganic nanoparticle and their applications.

Michel R, Gradzielski M - Int J Mol Sci (2012)

Bottom Line: Research on these systems has led to the observation of novel hybrid structures whose morphology strongly depends on the charge, composition and size of the interacting colloidal species as well as on the nature (pH, ionic strength) of their dispersing medium.A central role is played by the phase behaviour of phospholipid bilayers which have a tremendous influence on the liposome properties.Another central aspect is the incorporation of nanoparticles into vesicles, which is intimately linked to the conditions required for transporting a nanoparticle through a membrane.

View Article: PubMed Central - PubMed

Affiliation: Stranski-Laboratorium für Physikalische und Theoretische Chemie, Institut für Chemie, Technische Universität Berlin, Berlin D-10623, Germany; E-Mails: raphael.michel@mailbox.tu-berlin.de (R.M.); michael.gradzielski@tu-berlin.de (M.G.); Tel.: +49-30-314-22822 (R.M.); +49-30-314-24934 (M.G.); M.G.).

ABSTRACT
In the past few years, growing attention has been devoted to the study of the interactions taking place in mixed systems of phospholipid membranes (for instance in the form of vesicles) and hard nanoparticles (NPs). In this context liposomes (vesicles) may serve as versatile carriers or as a model system for biological membranes. Research on these systems has led to the observation of novel hybrid structures whose morphology strongly depends on the charge, composition and size of the interacting colloidal species as well as on the nature (pH, ionic strength) of their dispersing medium. A central role is played by the phase behaviour of phospholipid bilayers which have a tremendous influence on the liposome properties. Another central aspect is the incorporation of nanoparticles into vesicles, which is intimately linked to the conditions required for transporting a nanoparticle through a membrane. Herein, we review recent progress made on the investigations of the interactions in liposome/nanoparticle systems focusing on the particularly interesting structures that are formed in these hybrid systems as well as their potential applications.

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Related in: MedlinePlus

CryoTEM images of internalized silica hard nanoparticles (NPs) within DOPC (dioleylphosphatidylcholine) liposomes—visualization of the SLB on internalized particles (black arrow) and of “free” NP covered only with a SLB (asterisk). Scale bar 50 nm. Figure reprinted from [42].
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f4-ijms-13-11610: CryoTEM images of internalized silica hard nanoparticles (NPs) within DOPC (dioleylphosphatidylcholine) liposomes—visualization of the SLB on internalized particles (black arrow) and of “free” NP covered only with a SLB (asterisk). Scale bar 50 nm. Figure reprinted from [42].

Mentions: (2) In the second case mentioned previously, the SLB formation on smaller particles is accompanied by the internalization of the nanoparticle into the vesicle interior, as has for instance been observed for the case of silica NPs interacting with DOPC vesicles (Figure 4). As described by Le Bihan et al. [42], the proposed invagination mechanism predicts the adsorption of the particle on the liposome outer surface, followed by the spreading of the membrane around the particle finally leading to the engulfing and the full internalization of the nanoparticle. This process of nanoparticle transmigration is similar to that of cellular uptake (or endocytosis) [108,109]. Simulations on similar systems [110] have been found to consistently reproduce the invagination mechanism described above.


Experimental aspects of colloidal interactions in mixed systems of liposome and inorganic nanoparticle and their applications.

Michel R, Gradzielski M - Int J Mol Sci (2012)

CryoTEM images of internalized silica hard nanoparticles (NPs) within DOPC (dioleylphosphatidylcholine) liposomes—visualization of the SLB on internalized particles (black arrow) and of “free” NP covered only with a SLB (asterisk). Scale bar 50 nm. Figure reprinted from [42].
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472766&req=5

f4-ijms-13-11610: CryoTEM images of internalized silica hard nanoparticles (NPs) within DOPC (dioleylphosphatidylcholine) liposomes—visualization of the SLB on internalized particles (black arrow) and of “free” NP covered only with a SLB (asterisk). Scale bar 50 nm. Figure reprinted from [42].
Mentions: (2) In the second case mentioned previously, the SLB formation on smaller particles is accompanied by the internalization of the nanoparticle into the vesicle interior, as has for instance been observed for the case of silica NPs interacting with DOPC vesicles (Figure 4). As described by Le Bihan et al. [42], the proposed invagination mechanism predicts the adsorption of the particle on the liposome outer surface, followed by the spreading of the membrane around the particle finally leading to the engulfing and the full internalization of the nanoparticle. This process of nanoparticle transmigration is similar to that of cellular uptake (or endocytosis) [108,109]. Simulations on similar systems [110] have been found to consistently reproduce the invagination mechanism described above.

Bottom Line: Research on these systems has led to the observation of novel hybrid structures whose morphology strongly depends on the charge, composition and size of the interacting colloidal species as well as on the nature (pH, ionic strength) of their dispersing medium.A central role is played by the phase behaviour of phospholipid bilayers which have a tremendous influence on the liposome properties.Another central aspect is the incorporation of nanoparticles into vesicles, which is intimately linked to the conditions required for transporting a nanoparticle through a membrane.

View Article: PubMed Central - PubMed

Affiliation: Stranski-Laboratorium für Physikalische und Theoretische Chemie, Institut für Chemie, Technische Universität Berlin, Berlin D-10623, Germany; E-Mails: raphael.michel@mailbox.tu-berlin.de (R.M.); michael.gradzielski@tu-berlin.de (M.G.); Tel.: +49-30-314-22822 (R.M.); +49-30-314-24934 (M.G.); M.G.).

ABSTRACT
In the past few years, growing attention has been devoted to the study of the interactions taking place in mixed systems of phospholipid membranes (for instance in the form of vesicles) and hard nanoparticles (NPs). In this context liposomes (vesicles) may serve as versatile carriers or as a model system for biological membranes. Research on these systems has led to the observation of novel hybrid structures whose morphology strongly depends on the charge, composition and size of the interacting colloidal species as well as on the nature (pH, ionic strength) of their dispersing medium. A central role is played by the phase behaviour of phospholipid bilayers which have a tremendous influence on the liposome properties. Another central aspect is the incorporation of nanoparticles into vesicles, which is intimately linked to the conditions required for transporting a nanoparticle through a membrane. Herein, we review recent progress made on the investigations of the interactions in liposome/nanoparticle systems focusing on the particularly interesting structures that are formed in these hybrid systems as well as their potential applications.

Show MeSH
Related in: MedlinePlus