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Characterization of Erysiphe necator-responsive genes in Chinese Wild Vitis quinquangularis.

Gao M, Niu J, Zhao S, Jiao C, Xu W, Fei Z, Wang X - Int J Mol Sci (2012)

Bottom Line: Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library.Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection.The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China; E-Mails: gaomin.001@163.com (M.G.); niujiao.2009@163.com (J.N.); bashijiu@163.com (S.Z.); dd_race@sohu.com (C.J.) ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone "Shang-24". A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

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Related in: MedlinePlus

Real-time quantitative RT-PCR analysis of transcript accumulation in two grapevine genotypes in response to E. necator at 0, 6, 12, 24, 48, 72, 96, and 120 hpi. Data represents means of triplicate data. Expression profiles of genes in the disease/defense category (A); genes involved in Ubiquitin/26S proteasome pathway (B); genes in the metabolism category (C); and genes with no known homologs (D) are shown. Solid line, E. necator-inoculated samples; dashed line, mock-inoculated samples.
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f4-ijms-13-11497: Real-time quantitative RT-PCR analysis of transcript accumulation in two grapevine genotypes in response to E. necator at 0, 6, 12, 24, 48, 72, 96, and 120 hpi. Data represents means of triplicate data. Expression profiles of genes in the disease/defense category (A); genes involved in Ubiquitin/26S proteasome pathway (B); genes in the metabolism category (C); and genes with no known homologs (D) are shown. Solid line, E. necator-inoculated samples; dashed line, mock-inoculated samples.

Mentions: To further confirm the expression patterns of identified genes during the interaction between grapevine and E. necator at different time points, qRT-PCR was performed with 11 genes represented by one EST and two genes by two or more ESTs on E. necator- and mock-inoculated samples at eight time points (0, 6, 12, 24, 48, 72, 96, and 120 hpi). These 13 genes in different functional groups were selected for the qRT-PCR analysis because of defense-related roles of their homologs in hypersensitivity, ubiquitin/proteasome pathway and phenylpropanoid synthesis reported previously, and because of their novel sequences. Nine of them corresponded to three functional categories: metabolism (3 genes), protein synthesis and fate (3 genes) and response to defense (3 genes) (Table 1), and four had no match to any sequences in the GenBank nr database. Distinct expression patterns of these genes were observed in resistant and susceptible grapevines in response to E. necator and most of the selected genes were strongly elevated by E. necator only in the resistant material “Shang-24”, while not in the susceptible material “Hunan-1” (Figure 4).


Characterization of Erysiphe necator-responsive genes in Chinese Wild Vitis quinquangularis.

Gao M, Niu J, Zhao S, Jiao C, Xu W, Fei Z, Wang X - Int J Mol Sci (2012)

Real-time quantitative RT-PCR analysis of transcript accumulation in two grapevine genotypes in response to E. necator at 0, 6, 12, 24, 48, 72, 96, and 120 hpi. Data represents means of triplicate data. Expression profiles of genes in the disease/defense category (A); genes involved in Ubiquitin/26S proteasome pathway (B); genes in the metabolism category (C); and genes with no known homologs (D) are shown. Solid line, E. necator-inoculated samples; dashed line, mock-inoculated samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472759&req=5

f4-ijms-13-11497: Real-time quantitative RT-PCR analysis of transcript accumulation in two grapevine genotypes in response to E. necator at 0, 6, 12, 24, 48, 72, 96, and 120 hpi. Data represents means of triplicate data. Expression profiles of genes in the disease/defense category (A); genes involved in Ubiquitin/26S proteasome pathway (B); genes in the metabolism category (C); and genes with no known homologs (D) are shown. Solid line, E. necator-inoculated samples; dashed line, mock-inoculated samples.
Mentions: To further confirm the expression patterns of identified genes during the interaction between grapevine and E. necator at different time points, qRT-PCR was performed with 11 genes represented by one EST and two genes by two or more ESTs on E. necator- and mock-inoculated samples at eight time points (0, 6, 12, 24, 48, 72, 96, and 120 hpi). These 13 genes in different functional groups were selected for the qRT-PCR analysis because of defense-related roles of their homologs in hypersensitivity, ubiquitin/proteasome pathway and phenylpropanoid synthesis reported previously, and because of their novel sequences. Nine of them corresponded to three functional categories: metabolism (3 genes), protein synthesis and fate (3 genes) and response to defense (3 genes) (Table 1), and four had no match to any sequences in the GenBank nr database. Distinct expression patterns of these genes were observed in resistant and susceptible grapevines in response to E. necator and most of the selected genes were strongly elevated by E. necator only in the resistant material “Shang-24”, while not in the susceptible material “Hunan-1” (Figure 4).

Bottom Line: Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library.Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection.The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China; E-Mails: gaomin.001@163.com (M.G.); niujiao.2009@163.com (J.N.); bashijiu@163.com (S.Z.); dd_race@sohu.com (C.J.) ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone "Shang-24". A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

Show MeSH
Related in: MedlinePlus