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Characterization of Erysiphe necator-responsive genes in Chinese Wild Vitis quinquangularis.

Gao M, Niu J, Zhao S, Jiao C, Xu W, Fei Z, Wang X - Int J Mol Sci (2012)

Bottom Line: Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library.Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection.The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China; E-Mails: gaomin.001@163.com (M.G.); niujiao.2009@163.com (J.N.); bashijiu@163.com (S.Z.); dd_race@sohu.com (C.J.) ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone "Shang-24". A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

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Related in: MedlinePlus

Functional classification of V. quinquangularis “Shang-24” unigenes within (A) molecular function categories and (B) biological process categories, respectively.
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f3-ijms-13-11497: Functional classification of V. quinquangularis “Shang-24” unigenes within (A) molecular function categories and (B) biological process categories, respectively.

Mentions: The unigenes from E. necator-inoculated grapevine leaves were further assigned gene ontology (GO) terms. Based on the GO annotations, unigenes were classified into high-level plant specific GO slims within the two ontology categories, namely, molecular function and biological process. A total of 176 unigenes were assigned to 22 GO slims within the molecular function category and 188 unigenes to 41 GO slims within the biological process category (Figure 3). Within the molecular function category, catalytic activity and binding were the most abundant GO slims (Figure 3A). Response to stress was the most abundant GO slim within the biological process category, which contained responses to stress, abiotic stimulus, and chemical stimulus, which is consistent with the fact that the unigenes were derived from tissues subjected to E. necator inoculation in Chinese wild V. quinquangularis (Figure 3B).


Characterization of Erysiphe necator-responsive genes in Chinese Wild Vitis quinquangularis.

Gao M, Niu J, Zhao S, Jiao C, Xu W, Fei Z, Wang X - Int J Mol Sci (2012)

Functional classification of V. quinquangularis “Shang-24” unigenes within (A) molecular function categories and (B) biological process categories, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472759&req=5

f3-ijms-13-11497: Functional classification of V. quinquangularis “Shang-24” unigenes within (A) molecular function categories and (B) biological process categories, respectively.
Mentions: The unigenes from E. necator-inoculated grapevine leaves were further assigned gene ontology (GO) terms. Based on the GO annotations, unigenes were classified into high-level plant specific GO slims within the two ontology categories, namely, molecular function and biological process. A total of 176 unigenes were assigned to 22 GO slims within the molecular function category and 188 unigenes to 41 GO slims within the biological process category (Figure 3). Within the molecular function category, catalytic activity and binding were the most abundant GO slims (Figure 3A). Response to stress was the most abundant GO slim within the biological process category, which contained responses to stress, abiotic stimulus, and chemical stimulus, which is consistent with the fact that the unigenes were derived from tissues subjected to E. necator inoculation in Chinese wild V. quinquangularis (Figure 3B).

Bottom Line: Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library.Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection.The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China; E-Mails: gaomin.001@163.com (M.G.); niujiao.2009@163.com (J.N.); bashijiu@163.com (S.Z.); dd_race@sohu.com (C.J.) ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone "Shang-24". A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

Show MeSH
Related in: MedlinePlus