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Characterization of Erysiphe necator-responsive genes in Chinese Wild Vitis quinquangularis.

Gao M, Niu J, Zhao S, Jiao C, Xu W, Fei Z, Wang X - Int J Mol Sci (2012)

Bottom Line: Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library.Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection.The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China; E-Mails: gaomin.001@163.com (M.G.); niujiao.2009@163.com (J.N.); bashijiu@163.com (S.Z.); dd_race@sohu.com (C.J.) ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone "Shang-24". A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

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Related in: MedlinePlus

Progression of PM on V. quinquangularis clone “Shang-24” and V. pseudoreticulata clone “Hunan-1” leaves. Shown are representative images taken at 24, 48, and 120 hpi with conidiospores. Spores and hyphae were stained with aniline blue (0.1% v/v in lacto glycerol). H2O2 accumulation was indicated by brown staining due to DAB polymerization. Micrographs were obtained under transmitted white light. Bars show scale. Filled triangles mark epidermal cell browning beneath the appressorium; SP: conidiospore; GT: germ tube; AP: appressorium; SH: secondary hyphae.
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f1-ijms-13-11497: Progression of PM on V. quinquangularis clone “Shang-24” and V. pseudoreticulata clone “Hunan-1” leaves. Shown are representative images taken at 24, 48, and 120 hpi with conidiospores. Spores and hyphae were stained with aniline blue (0.1% v/v in lacto glycerol). H2O2 accumulation was indicated by brown staining due to DAB polymerization. Micrographs were obtained under transmitted white light. Bars show scale. Filled triangles mark epidermal cell browning beneath the appressorium; SP: conidiospore; GT: germ tube; AP: appressorium; SH: secondary hyphae.

Mentions: To investigate the developmental time-course of the pathogen in Chinese wild V. quinquangularis clone “Shang-24” and V. pseudoreticulata clone “Hunan-1”, we conducted a microscopy study of conidiospore germination and hyphal development during a 5-day time period. Microscopic images of 24, 48 and 120 hpi are presented in Figure 1.


Characterization of Erysiphe necator-responsive genes in Chinese Wild Vitis quinquangularis.

Gao M, Niu J, Zhao S, Jiao C, Xu W, Fei Z, Wang X - Int J Mol Sci (2012)

Progression of PM on V. quinquangularis clone “Shang-24” and V. pseudoreticulata clone “Hunan-1” leaves. Shown are representative images taken at 24, 48, and 120 hpi with conidiospores. Spores and hyphae were stained with aniline blue (0.1% v/v in lacto glycerol). H2O2 accumulation was indicated by brown staining due to DAB polymerization. Micrographs were obtained under transmitted white light. Bars show scale. Filled triangles mark epidermal cell browning beneath the appressorium; SP: conidiospore; GT: germ tube; AP: appressorium; SH: secondary hyphae.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472759&req=5

f1-ijms-13-11497: Progression of PM on V. quinquangularis clone “Shang-24” and V. pseudoreticulata clone “Hunan-1” leaves. Shown are representative images taken at 24, 48, and 120 hpi with conidiospores. Spores and hyphae were stained with aniline blue (0.1% v/v in lacto glycerol). H2O2 accumulation was indicated by brown staining due to DAB polymerization. Micrographs were obtained under transmitted white light. Bars show scale. Filled triangles mark epidermal cell browning beneath the appressorium; SP: conidiospore; GT: germ tube; AP: appressorium; SH: secondary hyphae.
Mentions: To investigate the developmental time-course of the pathogen in Chinese wild V. quinquangularis clone “Shang-24” and V. pseudoreticulata clone “Hunan-1”, we conducted a microscopy study of conidiospore germination and hyphal development during a 5-day time period. Microscopic images of 24, 48 and 120 hpi are presented in Figure 1.

Bottom Line: Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library.Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection.The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China; E-Mails: gaomin.001@163.com (M.G.); niujiao.2009@163.com (J.N.); bashijiu@163.com (S.Z.); dd_race@sohu.com (C.J.) ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone "Shang-24". A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.

Show MeSH
Related in: MedlinePlus