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Medium optimization for exopolysaccharide production in liquid culture of endophytic fungus Berkleasmium sp. Dzf12.

Li P, Xu L, Mou Y, Shan T, Mao Z, Lu S, Peng Y, Zhou L - Int J Mol Sci (2012)

Bottom Line: The concentrations of glucose, peptone and MgSO(4)·7H(2)O were found to be the main effective factors for EPS production by FFD experimental analysis.The results provide the support data for EPS production in large scale and also speed up the application of Berkleasmium sp.Dzf12.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: lipq110@163.com (P.L.); lbaf2006@163.com (L.X.); muyan01987@163.com (Y.M.); shan5400388@163.com (T.S.); maoziling2011@163.com (Z.M.); shiqionglu@126.com (S.L.); pengyl@cau.edu.cn (Y.P.) ; Department of Forestry Pathology, College of Forestry, Northwest A & F University, Yangling 712100, China.

ABSTRACT
Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, is a high producer of spirobisnaphthalenes with various bioactivities. The exopolysaccharide (EPS) produced by this fungus also shows excellent antioxidant activity. In this study, the experimental designs based on statistics were employed to evaluate and optimize the medium for EPS production in liquid culture of Berkleasmium sp. Dzf12. For increasing EPS yield, the concentrations of glucose, peptone, KH(2)PO(4), MgSO(4)·7H(2)O and FeSO(4)·7H(2)O in medium were optimized using response surface methodology (RSM). Both the fractional factorial design (FFD) and central composite design (CCD) were applied to optimize the main factors which significantly affected EPS production. The concentrations of glucose, peptone and MgSO(4)·7H(2)O were found to be the main effective factors for EPS production by FFD experimental analysis. Based on the further CCD optimization and RSM analysis, a quadratic polynomial regression equation was derived from the EPS yield and three variables. Statistical analysis showed the polynomial regression model was in good agreement with the experimental results with the determination coefficient (adj-R(2)) as 0.9434. By solving the quadratic regression equation, the optimal concentrations of glucose, peptone and MgSO(4)·7H(2)O for EPS production were determined as 63.80, 20.76 and 2.74 g/L, respectively. Under the optimum conditions, the predicted EPS yield reached the maximum (13.22 g/L). Verification experiment confirmed the validity with the actual EPS yield as 13.97 g/L, which was 6.29-fold in comparison with that (2.22 g/L) in the original basal medium. The results provide the support data for EPS production in large scale and also speed up the application of Berkleasmium sp. Dzf12.

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Related in: MedlinePlus

Effects of the concentrations (g/L) of glucose (A); peptone (B); and MgSO4·7H2O (C) in medium on exopolysaccharide (EPS) production in fermentation culture of Berkleasmium sp. Dzf12. The error bars represent standard deviations from three independent samples. Different letters indicate significant differences among the treatments at p = 0.05 level.
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f1-ijms-13-11411: Effects of the concentrations (g/L) of glucose (A); peptone (B); and MgSO4·7H2O (C) in medium on exopolysaccharide (EPS) production in fermentation culture of Berkleasmium sp. Dzf12. The error bars represent standard deviations from three independent samples. Different letters indicate significant differences among the treatments at p = 0.05 level.

Mentions: The effects of the concentration of glucose ranged from 10 to 80 g/L on EPS production are presented in Figure 1A. When the concentration of glucose was increased from 10 to 60 g/L, the EPS yield was obviously increased from 1.98 to 13.37 g/L. However, when the concentration of glucose was higher than 60 g/L, the EPS yield was decreased slightly. It indicated the highest amount of EPS was attained when the concentration of glucose was approximating the neighborhood of 60 g/L. Thus, 60 g/L of glucose was selected as the center point of CCD.


Medium optimization for exopolysaccharide production in liquid culture of endophytic fungus Berkleasmium sp. Dzf12.

Li P, Xu L, Mou Y, Shan T, Mao Z, Lu S, Peng Y, Zhou L - Int J Mol Sci (2012)

Effects of the concentrations (g/L) of glucose (A); peptone (B); and MgSO4·7H2O (C) in medium on exopolysaccharide (EPS) production in fermentation culture of Berkleasmium sp. Dzf12. The error bars represent standard deviations from three independent samples. Different letters indicate significant differences among the treatments at p = 0.05 level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472754&req=5

f1-ijms-13-11411: Effects of the concentrations (g/L) of glucose (A); peptone (B); and MgSO4·7H2O (C) in medium on exopolysaccharide (EPS) production in fermentation culture of Berkleasmium sp. Dzf12. The error bars represent standard deviations from three independent samples. Different letters indicate significant differences among the treatments at p = 0.05 level.
Mentions: The effects of the concentration of glucose ranged from 10 to 80 g/L on EPS production are presented in Figure 1A. When the concentration of glucose was increased from 10 to 60 g/L, the EPS yield was obviously increased from 1.98 to 13.37 g/L. However, when the concentration of glucose was higher than 60 g/L, the EPS yield was decreased slightly. It indicated the highest amount of EPS was attained when the concentration of glucose was approximating the neighborhood of 60 g/L. Thus, 60 g/L of glucose was selected as the center point of CCD.

Bottom Line: The concentrations of glucose, peptone and MgSO(4)·7H(2)O were found to be the main effective factors for EPS production by FFD experimental analysis.The results provide the support data for EPS production in large scale and also speed up the application of Berkleasmium sp.Dzf12.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: lipq110@163.com (P.L.); lbaf2006@163.com (L.X.); muyan01987@163.com (Y.M.); shan5400388@163.com (T.S.); maoziling2011@163.com (Z.M.); shiqionglu@126.com (S.L.); pengyl@cau.edu.cn (Y.P.) ; Department of Forestry Pathology, College of Forestry, Northwest A & F University, Yangling 712100, China.

ABSTRACT
Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, is a high producer of spirobisnaphthalenes with various bioactivities. The exopolysaccharide (EPS) produced by this fungus also shows excellent antioxidant activity. In this study, the experimental designs based on statistics were employed to evaluate and optimize the medium for EPS production in liquid culture of Berkleasmium sp. Dzf12. For increasing EPS yield, the concentrations of glucose, peptone, KH(2)PO(4), MgSO(4)·7H(2)O and FeSO(4)·7H(2)O in medium were optimized using response surface methodology (RSM). Both the fractional factorial design (FFD) and central composite design (CCD) were applied to optimize the main factors which significantly affected EPS production. The concentrations of glucose, peptone and MgSO(4)·7H(2)O were found to be the main effective factors for EPS production by FFD experimental analysis. Based on the further CCD optimization and RSM analysis, a quadratic polynomial regression equation was derived from the EPS yield and three variables. Statistical analysis showed the polynomial regression model was in good agreement with the experimental results with the determination coefficient (adj-R(2)) as 0.9434. By solving the quadratic regression equation, the optimal concentrations of glucose, peptone and MgSO(4)·7H(2)O for EPS production were determined as 63.80, 20.76 and 2.74 g/L, respectively. Under the optimum conditions, the predicted EPS yield reached the maximum (13.22 g/L). Verification experiment confirmed the validity with the actual EPS yield as 13.97 g/L, which was 6.29-fold in comparison with that (2.22 g/L) in the original basal medium. The results provide the support data for EPS production in large scale and also speed up the application of Berkleasmium sp. Dzf12.

Show MeSH
Related in: MedlinePlus