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Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

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Decolourization of Copper (II) phthalocyanine (CuP) dye with C. subvermispora enzyme mixture from OLM (●) and BWCM (○) at room temperature. Experimental error is in the range of ±4%.
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f9-ijms-13-11365: Decolourization of Copper (II) phthalocyanine (CuP) dye with C. subvermispora enzyme mixture from OLM (●) and BWCM (○) at room temperature. Experimental error is in the range of ±4%.

Mentions: Further, the mixture of ligninolytic enzymes produced by the white rot fungus C. subvermispora could be used in environmental applications, in the paper industry and also for the decolourization of synthetic dyes [1,4,15,17]. In our investigation, we tested the possible applicability of the ligninolytic enzyme mixture produced in BWCM and OLM for decolourization of the two structurally different dyes Remazol Brilliant Blue (RBBR) and Copper (II) phthalocyanine (CuP). The decolorization of RBBR was faster and more efficient than that of CuP with the enzyme mixture from BWCM (80% and 60% decolorization in approximately 8 and 12 h, respectively). We assumed that Lac played a crucial role in the decolorization of this dye (Figure 8). Our results show that CuP is more difficult to degrade with Lac than RBBR. This effect was already documented during a study of the fungus Dichomitus squalens [20]. A similar result, that decolorization of anthraquinonic dyes is easier with laccases produced by white rot fungi, was also reported in the work by Liu et al. [28]. The decolorization of CuP was initially faster than that of RBBR, probably due to higher MnP activities in the OLM. When filtrates with lower Lac activities and higher MnP were used for decolorization studies, such as the filtrate from OLM in this study, the compensation and cooperation of these two enzymes in the decolorization process could be implied. These findings were suggested during dye degradation studies with Lac and MnP from D. squalens [29]. The extent of decolorization after 14 h was 60% for both enzyme mixtures in BWCM and OLM (Figure 9). The results of our decolorization study with the C. subvermispora ligninolytic enzyme system show the potential use of this fungus for environmental protection and bioremediation of pollutants. However, in the future during the enzymatic degradation studies, more attention should be paid to the analysis of the products appearing during enzyme degradation.


Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Decolourization of Copper (II) phthalocyanine (CuP) dye with C. subvermispora enzyme mixture from OLM (●) and BWCM (○) at room temperature. Experimental error is in the range of ±4%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472751&req=5

f9-ijms-13-11365: Decolourization of Copper (II) phthalocyanine (CuP) dye with C. subvermispora enzyme mixture from OLM (●) and BWCM (○) at room temperature. Experimental error is in the range of ±4%.
Mentions: Further, the mixture of ligninolytic enzymes produced by the white rot fungus C. subvermispora could be used in environmental applications, in the paper industry and also for the decolourization of synthetic dyes [1,4,15,17]. In our investigation, we tested the possible applicability of the ligninolytic enzyme mixture produced in BWCM and OLM for decolourization of the two structurally different dyes Remazol Brilliant Blue (RBBR) and Copper (II) phthalocyanine (CuP). The decolorization of RBBR was faster and more efficient than that of CuP with the enzyme mixture from BWCM (80% and 60% decolorization in approximately 8 and 12 h, respectively). We assumed that Lac played a crucial role in the decolorization of this dye (Figure 8). Our results show that CuP is more difficult to degrade with Lac than RBBR. This effect was already documented during a study of the fungus Dichomitus squalens [20]. A similar result, that decolorization of anthraquinonic dyes is easier with laccases produced by white rot fungi, was also reported in the work by Liu et al. [28]. The decolorization of CuP was initially faster than that of RBBR, probably due to higher MnP activities in the OLM. When filtrates with lower Lac activities and higher MnP were used for decolorization studies, such as the filtrate from OLM in this study, the compensation and cooperation of these two enzymes in the decolorization process could be implied. These findings were suggested during dye degradation studies with Lac and MnP from D. squalens [29]. The extent of decolorization after 14 h was 60% for both enzyme mixtures in BWCM and OLM (Figure 9). The results of our decolorization study with the C. subvermispora ligninolytic enzyme system show the potential use of this fungus for environmental protection and bioremediation of pollutants. However, in the future during the enzymatic degradation studies, more attention should be paid to the analysis of the products appearing during enzyme degradation.

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

Show MeSH